Human Anti-L1CAM Recombinant Antibody (clone H2); Fab Fragment (CAT#: FAMAB-0192-YC-F(E))
Provided are antibodies against mouse L1 from a human synthetic phage display library. The antibodies recognized mouse, but not human L1, by enzyme-linked immunosorbent assay, Western blot, and immunofluorescence staining of cultured neurons. Epitope mapping showed reactivity with the fibronectin type III repeats 1–2 of mouse L1. The antibodies stimulated neurite outgrowth from cerebellar dorsal, root ganglion and motor neurons when offered in substrate-coated form in a dose-dependent manner with maximal effects at approximately 32 nM.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10896/208_G1_2_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10896/208_G1_2_selected.jpg
Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10896/207_G1_1_red_green.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10896/207_G1_1_red_green.jpg
Figure 3 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10896/206_G1_1_red_green.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10896/206_G1_1_red_green.jpg
Figure 4 Cerebral cortex
(Neuropil
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10896/27469_B_8_5.jpg
(Neuropil
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10896/27469_B_8_5.jpg
Figure 5 Colon
(Endothelial cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
Peripheral nerve/ganglion
Staining:
High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10896/27469_A_7_3.jpg
(Endothelial cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
Peripheral nerve/ganglion
Staining:
High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10896/27469_A_7_3.jpg
Figure 6 Kidney
(Collecting ducts
Staining:
High
Intensity: Strong
Quantity:>75%
Distal tubules
Staining:
High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10896/27469_A_8_5.jpg
(Collecting ducts
Staining:
High
Intensity: Strong
Quantity:>75%
Distal tubules
Staining:
High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10896/27469_A_8_5.jpg
Figure 7 RNA cell line category: Cell line enhanced (BJ hTERT+, BJ hTERT+ SV40 Large T+ RasG12V, HeLa, RH-30, RPTEC TERT1)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000198910-L1CAM
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000198910-L1CAM
Figure 7 RNA cell line category: Cell line enhanced (BJ hTERT+, BJ hTERT+ SV40 Large T+ RasG12V, HeLa, RH-30, RPTEC TERT1)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000198910-L1CAM
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000198910-L1CAM
Specifications
- Host Species
- Human
- Derivation
- Phage display library
- Type
- Human Fab
- Specificity
- Mouse L1CAM
- Species Reactivity
- Mouse
- Clone
- H2
- Applications
- ELISA, IF, WB
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Fab Antibody Products
Cancer Stem Cell Biomarker Specific Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0635CL | Human Anti-L1CAM Recombinant Antibody (TAB-0635CL) | WB, IF, IHC, PK, ELISA, FC | Human antibody |
| TAB-0635CL-S(P) | Human Anti-L1CAM Recombinant Antibody; scFv Fragment (TAB-0635CL-S(P)) | ELISA, FC | Human scFv |
| TAB-0635CL-F(E) | Human Anti-L1CAM Recombinant Antibody; Fab Fragment (TAB-0635CL-F(E)) | ELISA, FC | Human Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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