Human Anti-L1CAM Recombinant Antibody (clone G6)
CAT#: FAMAB-0191YC
Provided are antibodies against mouse L1 from a human synthetic phage display library. The antibodies recognized mouse, but not human L1, by enzyme-linked immunosorbent assay, Western blot, and immunofluorescence staining of cultured neurons. Epitope mapping showed reactivity with the fibronectin type III repeats 1–2 of mouse L1. The antibodies stimulated neurite outgrowth from cerebellar dorsal, root ganglion and motor neurons when offered in substrate-coated form in a dose-dependent manner with maximal effects at approximately 32 nM.
Gene Expression
Published Data
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10896/208_G1_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10896/207_G1_1_red_green.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10896/206_G1_1_red_green.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuropil
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10896/27469_B_8_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
Peripheral nerve/ganglion
Staining:
High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10896/27469_A_7_3.jpg
Normal Tissue
Figure 6 Kidney
Collecting ducts
Staining:
High
Intensity: Strong
Quantity:>75%
Distal tubules
Staining:
High
Intensity: Strong
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10896/27469_A_8_5.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (BJ hTERT+, BJ hTERT+ SV40 Large T+ RasG12V, HeLa, RH-30, RPTEC TERT1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000198910-L1CAM
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IF
Figure 1 Immunofluorescence of cerebellar granule neurons, L1 transfected L929 cells and parental L929 cells with L1 single-chain variable fragment (scFv) antibodies.
Bound scFv antibodies were visualized by using anti-myc antibody 9E10 followed by Cy3-conjugated goat anti-mouse IgG. The left panels (a tog) represent the corresponding phase-contrast micrographs to the right panels.
WB
Figure 2 Mapping of the L1 epitope recognized by the L1 single-chain variable fragment (scFv) antibodies.
The L1 fragments detected by G6.
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Specifications
- Host Species
- Human
- Derivation
- Phage display library
- Type
- Human IgG
- Specificity
- Mouse L1CAM
- Species Reactivity
- Mouse
- Clone
- G6
- Applications
- ELISA, IF, WB
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| TAB-0635CL-S(P) | Human Anti-L1CAM Recombinant Antibody; scFv Fragment (TAB-0635CL-S(P)) | ELISA, FC | Human scFv |
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| HPAB-0398-CN-F(E) | Mouse Anti-L1CAM Recombinant Antibody; Fab Fragment (HPAB-0398-CN-F(E)) | WB, ELISA, FC, IHC | Mouse Fab |
| FAMAB-0191-YC-F(E) | Human Anti-L1CAM Recombinant Antibody (clone G6); Fab Fragment | ELISA, IF, WB | Human Fab |
| FAMAB-0192-YC-F(E) | Human Anti-L1CAM Recombinant Antibody (clone H2); Fab Fragment | ELISA, IF, WB | Human Fab |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0191-YC-S(P) | Human Anti-L1CAM Recombinant Antibody (clone G6); scFv Fragment | ELISA, IF, WB | Human scFv |
| FAMAB-0192-YC-S(P) | Human Anti-L1CAM Recombinant Antibody (clone H2); scFv Fragment | ELISA, IF, WB | Human scFv |
| HPAB-0561-YJ-S(P) | Mouse Anti-L1CAM Recombinant Antibody (clone A10-A3); scFv Fragment | ELISA, FC, FuncS | Mouse scFv |
| HPAB-0562-YJ-S(P) | Mouse Anti-L1CAM Recombinant Antibody (clone cA10-A3); scFv Fragment | ELISA, FC, FuncS | Mouse scFv |
| HPAB-0563-YJ-S(P) | Human Anti-L1CAM Recombinant Antibody (clone HzA10A3-2.1); scFv Fragment | ELISA, FC, FuncS | Humanized scFv |
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