Mouse Anti-LMNA Monoclonal Antibody (VS7-0425-WR301)
CAT#: VS7-0425-WR301
This product is a mouse IgG antibody that binds to human LMNA. The antibody can be used for WB, IHC-P, IF, and ELISA assays. It cross reacts with mouse and rat.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line PC-3
Immunofluorescent staining of human cell line PC-3 shows localization to nuclear speckles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6523/1905_E12_1_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human epididymis
Immunohistochemical staining of human epididymis shows strong nuclear membrane positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6660/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human rectum
Immunohistochemical staining of human rectum shows strong nuclear membrane positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4022/ihc_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Glial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4022/12055_B_8_5.jpg
Normal Tissue
Figure 5 Colon
Endocrine cells
Staining: High
Intensity: Strong
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%
Enterocytes - Microvilli
Staining: High
Intensity: Strong
Fibroblasts
Staining: High
Intensity: Strong
Goblet cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Mucosal lymphoid cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4022/12055_A_9_3.jpg
Normal Tissue
Figure 6 Liver
Cholangiocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Hepatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4022/12055_A_7_4.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4022/12055_A_8_5.jpg
Normal Tissue
Figure 8 Testis
Leydig cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Peritubular cells
Staining: High
Intensity: Strong
Quantity:>75%
Sertoli cells
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4022/12055_A_6_6.jpg
Normal Tissue
Figure 9 Skin
Cells in basal layer
Staining: High
Intensity: Strong
Quantity:>75%
Cells in granular layer
Staining: High
Intensity: Strong
Quantity: 75%-25%
Cells in spinous layer
Staining: High
Intensity: Strong
Quantity: 75%-25%
Endothelial cells
Staining: High
Intensity: Strong
Fibrohistiocytic cells
Staining: High
Intensity: Strong
Langerhans cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4022/12055_B_9_1.jpg
Normal Tissue
Figure 10 Lymph node
Germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4022/12055_A_7_8.jpg
RNA Expression
Figure 11 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000160789-LMNA
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Specifications
- Immunogen
- Recombinant human LMNA fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human LMNA
- Species Reactivity
- Human, Mouse, Rat
- Molecule Class
- Type V Intermediate Filament Protein
- Applications
- WB, IHC-P, IF, ELISA
- Conjugate
- Unconjugated
- Related Disease
- Emery-Dreifuss muscular dystrophy 2, autosomal dominant (EDMD2); Emery-Dreifuss muscular dystrophy 3, autosomal recessive (EDMD3); Cardiomyopathy, dilated, 1A (CMD1A); Lipodystrophy, familial partial, 2 (FPLD2); Charcot-Marie-Tooth disease, axonal, 2B1 (CMT2B1); Hutchinson-Gilford progeria syndrome (HGPS); Cardiomyopathy, dilated, with hypergonadotropic hypogonadism (CMDHH); Mandibuloacral dysplasia with type A lipodystrophy (MADA); Restrictive dermopathy 2 (RSDM2); Heart-hand syndrome Slovenian type (HHS-Slovenian); Muscular dystrophy congenital LMNA-related (MDCL)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- 1 mg/ml
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry: 1:200 - 1:1000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- LMNA; LFP; Lamin A/C; Limb Girdle Muscular Dystrophy 1B (Autosomal Dominant); CMT2B1; Prelamin-A/C; Progeria 1 (Hutchinson-Gilford Type); LMN1; IDC; LGMD1B; Lamin; FPL; LMNC; FPLD; CDDC; CMD1A; CDCD1; LDP1; FPLD2; LMNL1; PRO1; 70 KDa Lamin; Lamin A/C-Like
- Gene ID
- 4000
- UniProt ID
- P02545
- Long Name
- Lamin A
- Cellular Localization
- Intermediate filament; Nucleus
- Post Translation Modifications
- Acetylation; Glycoprotein; Isopeptide bond; Lipoprotein; Methylation; Phosphoprotein; Prenylation; Ubl conjugation
- Protein Refseq
- NP_001244303.1
- Function
- Lamin-A/C; Lamins are intermediate filament proteins that assemble into a filamentous meshwork, and which constitute the major components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane;; Lamins provide a framework for the nuclear envelope, bridging the nuclear envelope and chromatin, thereby playing an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics;; Lamin A and C also regulate matrix stiffness by conferring nuclear mechanical properties .; The structural integrity of the lamina is strictly controlled by the cell cycle, as seen by the disintegration and formation of the nuclear envelope in prophase and telophase, respectively;; Lamin A and C are present in equal amounts in the lamina of mammals. Also invoved in DNA repair: recruited by DNA repair proteins XRCC4 and IFFO1 to the DNA double-strand breaks (DSBs) to prevent chromosome translocation by immobilizing broken DNA ends;; Required for normal development of peripheral nervous system and skeletal muscle and for muscle satellite cell proliferation. Required for osteoblastogenesis and bone formation;; Also prevents fat infiltration of muscle and bone marrow, helping to maintain the volume and strength of skeletal muscle and bone. Required for cardiac homeostasis.; Prelamin-A/C; Prelamin-A/C can accelerate smooth muscle cell senescence (PubMed:20458013).; It acts to disrupt mitosis and induce DNA damage in vascular smooth muscle cells (VSMCs), leading to mitotic failure, genomic instability, and premature senescence
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "LMNA"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1798z | Mouse Anti-LMNA Recombinant Antibody (clone 12B7) | ICC, IF, IHC | Mouse IgG3 |
| ZG-0446U | Rabbit Anti-LMNA Recombinant Antibody (clone 4H7) | ELISA, IHC, IF, FC, IP | Rabbit IgG |
| VS3-QX693 | Mouse Anti-LMNA Recombinant Antibody (clone 3D6-F4-B7) | WB | Mouse IgG2a |
| VS3-CJ299 | Mouse Anti-LMNA Recombinant Antibody (VS3-CJ299) | WB | Mouse IgG1, κ |
| VS3-XY1013 | Mouse Anti-LMNA Recombinant Antibody (clone 4E7) | ELISA, WB, IHC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0327MZ | Chicken Anti-LMNA Polyclonal IgY | WB | Chicken antibody |
| BRD-1186MZ | Chicken Anti-Lamin A/C Polyclonal IgY | N/A | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2067 | Hi-Affi™ Recombinant Rabbit Anti-LMNA Monoclonal Antibody (DS2067AB) | FC, ICC, IF, IHC-P, IP, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC1922 | PE-A*02:01/Human LMNA (RLAVYIDRV) MHC Tetramer | FCM | |
| MHC-CN0129 | APC-B*58:01/Human Lamin (KAGQVVTIW) MHC Tetramer | FCM | |
| MHC-CN0130 | PE-B*58:01/Human Lamin (KAGQVVTIW) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY175 | AbPlus™ Anti-LMNA Magnetic Beads (13A4D4) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC665 | CytoStream™ Rabbit Anti-LMNA Recombinant Antibody (VS13-YC665) | WB, ICC, IF, IHC-P, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4051 | Anti-LMNA Immunohistochemistry Kit | IHC | |
| VS-0525-XY4052 | Anti-Mouse LMNA Immunohistochemistry Kit | IHC |
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