Mouse Anti-LRRC15 Recombinant Antibody; Fab Fragment (HPAB-0235-WJ-F(E))

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Reliable in Flow Cytometry

In flow cytometry applications, this antibody demonstrates excellent reliability. It provides clear and consistent staining, which is crucial for detailed cell analysis in cancer studies.

Effective in Immunohistochemistry

Creative Biolabs' mouse anti-LRRC15 recombinant antibody is highly effective in immunohistochemistry. It delivers strong and specific staining, making it an invaluable tool for tissue analysis and cancer research.

Q&As

1. What is the protocol for preparing mouse anti-LRRC15 recombinant antibody for an ELISA assay?

   A: To prepare the antibody for an ELISA assay, dilute it to the recommended concentration using an ELISA coating buffer. Coat the wells with the antibody, block non-specific binding sites with a blocking buffer, and then proceed with the standard ELISA protocol for detection.

2. How can the mouse anti-LRRC15 recombinant antibody be used to study protein-protein interactions?

   A: The antibody can be used in co-immunoprecipitation (co-IP) assays to study protein-protein interactions. Incubate cell lysates with the antibody to capture LRRC15 and its interacting partners, followed by analysis through SDS-PAGE and Western blotting.

3. What are the recommended blocking conditions when using mouse anti-LRRC15 recombinant antibody in immunohistochemistry?

   A: Use a blocking buffer containing 5% BSA or non-fat dry milk to block non-specific binding sites. Incubate the tissue sections with the blocking buffer for at least 30 minutes before applying the primary antibody.

4. What are the considerations for using mouse anti-LRRC15 recombinant antibody in multiplex assays?

   A: Ensure that the antibody is compatible with other antibodies and detection systems used in the multiplex assay. Validate the antibody's performance in a multiplex format and optimize the concentrations to prevent cross-reactivity and interference.

5. How should cell samples be prepared for flow cytometry using mouse anti-LRRC15 recombinant antibody?

   A: Prepare single-cell suspensions and incubate them with the antibody at the recommended concentration. Use appropriate secondary antibodies and wash the cells thoroughly to reduce background staining. Analyze the samples using a flow cytometer.

View the frequently asked questions answered by Creative Biolabs Support.

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

MSDS

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