What is the isotype of this mouse antibody?

This recombinant mouse antibody is of the IgG1 isotype. It specifically recognizes LRRC15 (Leucine Rich Repeat Containing 15) and is produced in mammalian cells with chemically defined media.

What applications has this antibody been validated for?

This antibody has been validated for use in Enzyme-linked Immunosorbent Assay (ELISA) and Flow Cytometry (FC). It is suitable for detecting Human LRRC15 in these experimental setups.

Mouse Anti-LRRC15 Recombinant Antibody (HPAB-0235-WJ)

CAT#: HPAB-0235-WJ

This product is a recombinant mouse antibody that recognizes LRRC15. The antibody was expressed in mammalian cells with chemically defined culture media and was purified by affinity chromatography.

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & vesicles.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35503/412_C1_2_selected.jpg

Subcellular Location and Protein Expression Figure 2 IHC staining of human cerebellum

Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35503/ihc_selected.jpg

Normal Tissue Figure 3 Cerebral cortex

Glial cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
Neuropil
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35503/89507_B_9_5.jpg

Normal Tissue Figure 4 Colon

Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35503/89507_A_9_3.jpg

Normal Tissue Figure 5 Liver

Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35503/89507_A_7_4.jpg

Normal Tissue Figure 6 Kidney

Cells in tubules
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35503/89507_A_8_5.jpg

Normal Tissue Figure 7 Testis

Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35503/89507_A_6_6.jpg

Normal Tissue Figure 8 Lymph node

Non-germinal center cells
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35503/89507_A_9_8.jpg

RNA Expression Figure 9 RNA cell line category: Group enriched (fHDF/TERT166, U-87 MG)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000172061-LRRC15

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Specifications

Host Species
Mouse

Type
Mouse IgG1

Specificity
Human LRRC15

Species Reactivity
Human

Applications
ELISA, FC

Related Disease
Cancers

Product Property

Purity
>95% as determined by SDS-PAGE

Concentration
Please refer to the vial label for the specific concentration.

Buffer
PBS

Preservative
No preservatives

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Alternative Names
leucine rich repeat containing 15; LIB; leucine-rich repeat-containing protein 15; hLib; leucine-rich repeat protein induced by beta amyloid; leucine-rich repeat protein induced by beta-amyloid homolog

Gene ID
131578

UniProt ID
Q8TF66

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Breast cancer biomarkers at key points during disease progression

Validated For Flow Cytometry

We used this mouse recombinant antibody to detect LRRC15 expression on cell surfaces via Flow Cytometry. The signal-to-noise ratio was very good. It is a specific IgG1 antibody that helped us investigate the leucine-rich repeat-containing protein 15 in our cancer research cell lines.

Pure Antibody For ELISA Applications

This antibody performed well in our ELISA screening for LRRC15. The product was chemically defined and purified by affinity chromatography, ensuring>95% purity. We found the batch consistency to be reliable, which is critical when running multiple plates over time for our collagen binding studies.

Q&As

What is the isotype of this mouse antibody?

A: This recombinant mouse antibody is of the IgG1 isotype. It specifically recognizes LRRC15 (Leucine Rich Repeat Containing 15) and is produced in mammalian cells with chemically defined media.
What applications has this antibody been validated for?

A: This antibody has been validated for use in Enzyme-linked Immunosorbent Assay (ELISA) and Flow Cytometry (FC). It is suitable for detecting Human LRRC15 in these experimental setups.

View the frequently asked questions answered by Creative Biolabs Support.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# HPAB-0235-WJ, RRID: AB_3713803)

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - HPAB-0235-WJ.pdf

MSDS

MSDS - HPAB-0235-WJ.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

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HPAB-0232-WJ-S(P)	Human Anti-LRRC15 Recombinant Antibody; scFv Fragment (HPAB-0232-WJ-S(P))	ELISA, FC	Humanized scFv

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HPAB-0230-WJ-F(E)	Human Anti-LRRC15 Recombinant Antibody; Fab Fragment (HPAB-0230-WJ-F(E))	ELISA, FC	Humanized Fab

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HPAB-0231-WJ-F(E)	Human Anti-LRRC15 Recombinant Antibody; Fab Fragment (HPAB-0231-WJ-F(E))	ELISA, FC	Humanized Fab

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HPAB-0232-WJ-F(E)	Human Anti-LRRC15 Recombinant Antibody; Fab Fragment (HPAB-0232-WJ-F(E))	ELISA, FC	Humanized Fab

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