SmartAb™ Recombinant Anti-MAPK1 pH-dependent Antibody (Clone VB22B)
CAT#: VS-0825-YC247
The anti-MAPK1 pH-dependent antibody (clone VB22B) is a recombinant monoclonal antibody that exhibits pH-dependent antigen binding to human Mitogen-activated protein kinase scaffold protein 1 (MAPK1). By introducing histidine mutations that act as a pH-switch, the antibody demonstrates a preferential affinity for MAPK1 under acidic conditions compared to physiological pH. Using a yeast surface display system with pH-dependent screening pressure, this mAb is identified which shows a significant increase in dissociation from MAPK1 at pH 6.0. This pH-sensitive antibody retains its binding affinity on tumor cells while exhibiting negligible binding to the receptor on normal cells at physiological pH. Human MAPK1, also known as ERK2, is a key kinase in the MAPK/ERK signaling pathway that regulates cell proliferation, differentiation, and survival. It is an important oncogene and a potential therapeutic target in cancer.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79930/1999_H7_3_selected.jpg
Subcellular Location
Figure 2 IHC staining of human cerebral cortex
Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic and nuclear positivity in neuronal and glial cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3995/12643_B_9_5_selected.jpg
Subcellular Location
Figure 3 IHC staining of human hippocampus
Immunohistochemical staining of human hippocampus shows strong cytoplasmic positivity in neuronal cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5700/21384_B_7_6_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
Glial cells
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
Neuronal cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5700/21384_B_8_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Glandular cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5700/20815_A_9_3.jpg
Normal Tissue
Figure 6 Liver
Cholangiocytes
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Hepatocytes
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5700/20815_A_7_4.jpg
Normal Tissue
Figure 7 Pancreas
Exocrine glandular cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Pancreatic endocrine cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5700/20815_A_2_3.jpg
Normal Tissue
Figure 8 Kidney
Cells in glomeruli
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
Cells in tubules
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5700/20815_A_9_5.jpg
Normal Tissue
Figure 9 Testis
Cells in seminiferous ducts
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
Leydig cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5700/20815_A_6_6.jpg
Normal Tissue
Figure 10 Lymph node
Germinal center cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Non-germinal center cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5700/20815_A_9_8.jpg
RNA Expression
Figure 11 RNA cell line category: Cell line enhanced (K-562)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000100030-MAPK1
❮
❯
Specifications
- Immunogen
- Human MPL protein with Flag tag.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human Mitogen-activated protein kinase scaffold protein 1
- Species Reactivity
- Human
- Clone
- VB22B
- Applications
- ELISA
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE and SEC-HPLC
- Buffer
- PBS, pH 7.4
- Preservative
- No preservatives
- Storage
- For short-term storage, keep it at 4°C for up to two weeks. For long-term storage, divide the solution into aliquots and freeze them at -20°C or below.
Target
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS-0825-YC247. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Related Signaling Pathways
FcεR1 Signaling Pathway
TCR Signaling Pathway
Related Diseases
Maturity Onset Diabetes of the Young
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Clone VB22B"
- CAT
- Product Name
See other products for "MAPK1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0829CL | Anti-Human MAPK1 Recombinant Antibody (VB22B) | ELISA | |
| TAB-0829CL-S(P) | Anti-Human MAPK1 Recombinant Antibody scFv Fragment (VB22B) | ELISA | |
| TAB-0829CL-F(E) | Anti-Human MAPK1 Recombinant Antibody Fab Fragment (VB22B) | ELISA |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0341MZ | Chicken Anti-MAPK1 Polyclonal IgY | WB | Chicken antibody |
| BRD-0899MZ | Chicken Anti-ERK2 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC208 | PE-H-2Kd/Human Erk2 K136Q (QYIHSANVL) MHC Tetramer | FCM | |
| MHC-LC209 | APC-H-2Kd/Human Erk2 K136Q (QYIHSANVL) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2148 | Hi-Affi™ Recombinant Rabbit Anti-MAPK1 Monoclonal Antibody (DS2148AB) | WB, IHC-P, ICC, FC, IP | IgG |
| MOR-2155 | Hi-Affi™ Recombinant Rabbit Anti-MAPK3; MAPK1 Monoclonal Antibody (DS2155AB) | WB, IHC-P, ICC, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0547-CN | Recombinant Mouse Anti-MAPK1 Monoclonal Antibody (5-D2) | WB, IF, IHC, FC | Mouse IgG1 |
| MRO-0548-CN | Recombinant Rabbit Anti-MAPK1 Monoclonal Antibody (CBACN-218) | WB, IF, IHC, IP, FC | Rabbit IgG |
| ZG-0455U | Rabbit Anti-MAPK1 Recombinant Antibody (clone 10C7) | ELISA, WB, IHC, IF | Rabbit IgG |
| VS3-QX721 | Mouse Anti-MAPK1 Recombinant Antibody (clone 10C8-9F6-10C8) | IHC | Mouse IgG1 |
| VS3-QX722 | Mouse Anti-MAPK1 Recombinant Antibody (clone 1H4-6D7-9D8) | IHC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1354 | AbPlus™ Anti-MAPK1 Magnetic Beads (VS-0724-YC1354) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY191 | Mouse Anti-NHP MAPK1 Recombinant Antibody (clone 4C11) | WB, IHC, IF, ELISA | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC691 | CytoStream™ Rabbit Anti-MAPK1 Recombinant Antibody (VS13-YC691) | WB, ICC, IF, IHC-P, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4224 | Anti-MAPK1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4225 | Anti-Mouse MAPK1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4226 | Anti-Canine MAPK1 Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: WB, FC, IP, ELISA, Neut, FuncS, IF
Application:
Application: FC, FRET, Internalization
Application: WB, ELISA, FuncS, Inhib, PK, IP, SPR
Application: ELISA, IHC, FC, IP, IF, Inhib
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: ELISA, FC, Neut, Inhib
Application: ELISA, FuncS
Application: ELISA, WB, IF, FC, IP
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
