CytoStream™ Rabbit Anti-MAPK1 Recombinant Antibody (VS13-YC691)
CAT#: VS13-YC691
The CytoStream™ Rabbit Anti-MAPK1 Recombinant Antibody is specific for human, mouse and rat MAPK1. MAPK1, part of MAP kinase family, integrates signals affecting proliferation, differentiation, and transcription with kinase activity independent transcript variants reporting mechanistic functions. The anti-MAPK1 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79930/1999_H7_3_selected.jpg
Subcellular Location
Figure 2 IHC staining of human cerebral cortex
Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic and nuclear positivity in neuronal and glial cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3995/12643_B_9_5_selected.jpg
Subcellular Location
Figure 3 IHC staining of human hippocampus
Immunohistochemical staining of human hippocampus shows strong cytoplasmic positivity in neuronal cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5700/21384_B_7_6_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
Glial cells
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
Neuronal cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5700/21384_B_8_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Glandular cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5700/20815_A_9_3.jpg
Normal Tissue
Figure 6 Liver
Cholangiocytes
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Hepatocytes
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5700/20815_A_7_4.jpg
Normal Tissue
Figure 7 Pancreas
Exocrine glandular cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Pancreatic endocrine cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5700/20815_A_2_3.jpg
Normal Tissue
Figure 8 Kidney
Cells in glomeruli
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
Cells in tubules
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5700/20815_A_9_5.jpg
Normal Tissue
Figure 9 Testis
Cells in seminiferous ducts
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
Leydig cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5700/20815_A_6_6.jpg
Normal Tissue
Figure 10 Lymph node
Germinal center cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Non-germinal center cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5700/20815_A_9_8.jpg
RNA Expression
Figure 11 RNA cell line category: Cell line enhanced (K-562)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000100030-MAPK1
❮
❯
Specifications
- Immunogen
- Recombinant human ERK2, aa 311-360.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat MAPK1
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, ICC, IF, IHC-P, IP, FC
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/5000
Immunocytochemistry (ICC): 1/50-1/200
Immunofluorescence (IF): 1/50-1/200
Immunohistochemistry-paraffin (IHC-P): 1/50-1/200
Flow cytometry (FC): 1/50-1/100
Target
- Alternative Names
- Mitogen-Activated Protein Kinase 1; Extracellular Signal-Regulated Kinase 2; Mitogen-Activated Protein Kinase 2; MAP Kinase Isoform P42; MAP Kinase 1; MAP Kinase 2; EC 2.7.11.24; P42-MAPK; MAPK 2; PRKM1; PRKM2; ERK-2; ERK2;
- Gene ID
- 5594
- UniProt ID
- P28482
- Long Name
- Mitogen-Activated Protein Kinase 1
- Cellular Localization
- Nucleus; Cytoplasm; Cytoskeleton; Cell junction; Cell membrane
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "MAPK1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0829CL | Anti-Human MAPK1 Recombinant Antibody (VB22B) | ELISA | |
| TAB-0829CL-S(P) | Anti-Human MAPK1 Recombinant Antibody scFv Fragment (VB22B) | ELISA | |
| TAB-0829CL-F(E) | Anti-Human MAPK1 Recombinant Antibody Fab Fragment (VB22B) | ELISA |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0341MZ | Chicken Anti-MAPK1 Polyclonal IgY | WB | Chicken antibody |
| BRD-0899MZ | Chicken Anti-ERK2 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC208 | PE-H-2Kd/Human Erk2 K136Q (QYIHSANVL) MHC Tetramer | FCM | |
| MHC-LC209 | APC-H-2Kd/Human Erk2 K136Q (QYIHSANVL) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2148 | Hi-Affi™ Recombinant Rabbit Anti-MAPK1 Monoclonal Antibody (DS2148AB) | WB, IHC-P, ICC, FC, IP | IgG |
| MOR-2155 | Hi-Affi™ Recombinant Rabbit Anti-MAPK3; MAPK1 Monoclonal Antibody (DS2155AB) | WB, IHC-P, ICC, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0547-CN | Recombinant Mouse Anti-MAPK1 Monoclonal Antibody (5-D2) | WB, IF, IHC, FC | Mouse IgG1 |
| MRO-0548-CN | Recombinant Rabbit Anti-MAPK1 Monoclonal Antibody (CBACN-218) | WB, IF, IHC, IP, FC | Rabbit IgG |
| ZG-0315J | Mouse Anti-MAPK1 Recombinant Antibody (ZG-0315J) | WB, IHC, IF, ELISA | Mouse IgG |
| VS7-0425-WR346 | Mouse Anti-MAPK1 Monoclonal Antibody (VS7-0425-WR346) | WB, IHC-P, IF, FC, ELISA | Mouse IgG |
| VS7-0425-WR549 | Mouse Anti-MAPK1 Monoclonal Antibody (clone 3F8) | WB, IHC, FC | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1354 | AbPlus™ Anti-MAPK1 Magnetic Beads (VS-0724-YC1354) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY191 | Mouse Anti-NHP MAPK1 Recombinant Antibody (clone 4C11) | WB, IHC, IF, ELISA | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4224 | Anti-MAPK1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4225 | Anti-Mouse MAPK1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4226 | Anti-Canine MAPK1 Immunohistochemistry Kit | IHC |
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