Mouse Anti-MAPK14 Recombinant Antibody (clone 8G4D11)
CAT#: VS7-0425-WR352
This product is a mouse IgG1 antibody that binds to human MAPK14. The antibody 8G4D11 recognizes specific amino acid domain of human MAPK14 (AA: 299-360). It can be used for WB, IHC, ICC, and FC assays.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51825/834_A12_2_selected.jpg
Normal Tissue
Figure 2 Lymph node
Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40578/149032_A_8_8.jpg
Normal Tissue
Figure 3 Liver
Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40578/149032_A_7_4.jpg
Normal Tissue
Figure 4 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40578/149032_A_8_5.jpg
Normal Tissue
Figure 5 Testis
Sertoli cells Staining: Medium Intensity: Moderate Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40578/149032_A_6_6.jpg
Normal Tissue
Figure 6 Bone marrow
Hematopoietic cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40578/149032_B_5_4.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000112062-MAPK14
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Specifications
- Immunogen
- Recombinant human MAPK14 (AA: 299-360) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human MAPK14
- Species Reactivity
- Human
- Clone
- 8G4D11
- Molecule Class
- Serine/Threonine Kinase
- Applications
- WB, IHC, ICC, FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Immunocytochemistry: 1:75
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- RK; p38; CSBP; EXIP; Mxi2; CSBP1; CSBP2; CSPB1; PRKM14; PRKM15; SAPK2A; P38alpha
- Gene ID
- 1432
- UniProt ID
- Q16539
- Long Name
- Mitogen-Activated Protein Kinase 14
- Cellular Localization
- Cytoplasm; Nucleus
- Post Translation Modifications
- Acetylation; Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_001306.1
- Function
- Kinase; Serine/threonine-protein kinase; Transferase
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Related Signaling Pathways
FcεR1 Signaling Pathway
TCR Signaling Pathway
Related Diseases
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
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