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    Mouse Anti-MAPK9 Recombinant Antibody (clone 4D2) (CAT#: VS3-WK1606)

    This antibody is a mouse recombinant antibody against human MAPK9, which targets specific transcription factors, and thus mediates immediate-early gene expression in response to various cell stimuli. The clone 4D2 can be used in IHC analysis at a dilution of 1/50-1/200.

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    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
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    Purity :
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    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location
    Figure 1 IF staining of human cell line EFO-21
    Figure 1 IF staining of human cell line EFO-21
    (Immunofluorescent staining of human cell line EFO-21 shows localization to nuclear speckles, plasma membrane & cytosol.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/72462/2063_E4_3_selected.jpg
    Normal Tissue
    Figure 2 Cerebral cortex
    Figure 2 Cerebral cortex
    (Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/8910/21890_B_7_5.jpg
    Figure 3 Colon
    Figure 3 Colon
    (Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/8910/21890_A_9_3.jpg
    Figure 4 Kidney
    Figure 4 Kidney
    (Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/8910/21890_A_7_5.jpg
    Figure 5 Testis
    Figure 5 Testis
    (Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/8910/21890_A_4_6.jpg
    Figure 6 Lymph node
    Figure 6 Lymph node
    (Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/8910/21890_A_8_8.jpg
    RNA Expression
    Figure 7 RNA cell line category: Cell line enhanced (HDLM-2)
    Figure 7 RNA cell line category: Cell line enhanced (HDLM-2)
    (Cell lines ordered by descending RNA expression order)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000050748-MAPK9

    Specifications

    • Immunogen
    • Synthesized peptide derived from human JNK2
    • Host Species
    • Mouse
    • Type
    • Mouse IgG
    • Specificity
    • Human MAPK9
    • Species Reactivity
    • Human, Mouse, Rat
    • Clone
    • 4D2
    • Applications
    • IHC
    • Conjugate
    • Unconjugated

    Product Property

    • Clonality
    • Monoclonal
    • Purification
    • Protein G affinity purified
    • Purity
    • >95% as determined by SDS-PAGE
    • Aggregation
    • < 5%
    • Format
    • Liquid
    • Concentration
    • 1 mg/mL
    • Buffer
    • PBS, 0.5% BSA, 50% Glycerol
    • Preservative
    • 0.02% Sodium Azide
    • Storage
    • Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
    • Shipping
    • Ice packs

    Applications

    • Application Notes
    • IHC: 1/50-1/200

    Target

    • Alternative Names
    • Mitogen-Activated Protein Kinase 9; Stress-Activated Protein Kinase JNK2; Stress-Activated Protein Kinase 1a; C-Jun N-Terminal Kinase 2; MAP Kinase 9; EC 2.7.11.24; Jun Kinase; MAPK 9; JNK-55; SAPK1a; PRKM9
    • Long Name
    • Mitogen-Activated Protein Kinase 9
    • Sequence Similarities
    • Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    • Cellular Localization
    • Cytoplasm, Nucleus
    • Post Translation Modifications
    • Dually phosphorylated on Thr-183 and Tyr-185 by MAP2K7 and MAP2K4, which activates the enzyme. Autophosphorylated in vitro.
    • Function
    • Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as pro-inflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK9/JNK2. In turn, MAPK9/JNK2 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN and ATF2 and thus regulates AP-1 transcriptional activity.

    Recommended Products

    Secondary Antibody

    Isotype Control

    Related Resources

    • Related Signaling Pathways
    • Related Diseases

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

    See other products for "MAPK9"

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    MOB-3199z Mouse Anti-MAPK9 Recombinant Antibody (clone 37H7) ELISA, IHC, WB Mouse IgG1, κ
    MOB-1001CT Recombinant Mouse anti-Human MAPK9 Monoclonal antibody (EML1864) WB
    VS3-CJ970 Rabbit Anti-MAPK9 Recombinant Antibody (VS3-CJ970) WB, ICC, IHC, IP, FC Rabbit IgG
    VS-0923-FY115 Recombinant Mouse Anti-MAPK9 Antibody (VS-0923-FY115) ELISA, WB Mouse IgG2a
    VS7-0425-WR359 Mouse Anti-MAPK9 Recombinant Antibody (clone 2F6H11) WB, FC Mouse IgG1

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