Rabbit Anti-MAPK9 Recombinant Antibody (VS3-CJ970)
CAT#: VS3-CJ970
This product is a rabbit antibody that recognizes human, mouse, and rat MAPK9.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line EFO-21
Immunofluorescent staining of human cell line EFO-21 shows localization to nuclear speckles, plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/72462/2063_E4_3_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8910/21890_B_7_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8910/21890_A_9_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8910/21890_A_7_5.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8910/21890_A_4_6.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8910/21890_A_8_8.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (HDLM-2)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000050748-MAPK9
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Specifications
- Immunogen
- Recombinant protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat MAPK9
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, ICC, IHC, IP, FC
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 40% Glycerol, 1% BSA, TBS, pH7.4.
- Preservative
- 0.05% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Western Blot, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Flow Cytometry.
Target
- Alternative Names
- JNK2; SAPK; p54a; JNK2A; JNK2B; PRKM9; JNK-55; SAPK1a; JNK2BETA; p54aSAPK; JNK2ALPHA
- Gene ID
- 5601
- UniProt ID
- P45984
- Sequence Similarities
- Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Dually phosphorylated on Thr-183 and Tyr-185 by MAP2K7 and MAP2K4, which activates the enzyme. Autophosphorylated in vitro.
- Protein Refseq
- NP_001128516.1; NP_001295173.1; NP_002743.3
- Function
- Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as pro-inflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK9/JNK2. In turn, MAPK9/JNK2 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN and ATF2 and thus regulates AP-1 transcriptional activity. In response to oxidative or ribotoxic stresses, inhibits rRNA synthesis by phosphorylating and inactivating the RNA polymerase 1-specific transcription initiation factor RRN3. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including TP53 and YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Upon T-cell receptor (TCR) stimulation, is activated by CARMA1, BCL10, MAP2K7 and MAP3K7/TAK1 to regulate JUN protein levels. Plays an important role in the osmotic stress-induced epithelial tight-junctions disruption. When activated, promotes beta-catenin/CTNNB1 degradation and inhibits the canonical Wnt signaling pathway. Participates also in neurite growth in spiral ganglion neurons. Phosphorylates the CLOCK-ARNTL/BMAL1 heterodimer and plays a role in the regulation of the circadian clock (PubMed:22441692).
Phosphorylates POU5F1, which results in the inhibition of POU5F1's transcriptional activity and enhances its proteosomal degradation (By similarity).
MAPK9 isoforms display different binding patterns: alpha-1 and alpha-2 preferentially bind to JUN, whereas beta-1 and beta-2 bind to ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. JUNB is not a substrate for JNK2 alpha-2, and JUND binds only weakly to it.
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Related Signaling Pathways
FcεR1 Signaling Pathway
TCR Signaling Pathway
Related Diseases
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "MAPK9"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3199z | Mouse Anti-MAPK9 Recombinant Antibody (clone 37H7) | ELISA, IHC, WB | Mouse IgG1, κ |
| MOB-1001CT | Recombinant Mouse anti-Human MAPK9 Monoclonal antibody (EML1864) | WB | |
| MRO-0885-CN | Recombinant Rabbit Anti-MAPK9 Monoclonal Antibody (CBACN-331) | WB, IF, IHC, IP, FC | Rabbit IgG |
| VS3-FY892 | Recombinant Rabbit Anti-MAPK9 Antibody (clone R02-9H4) | WB, IHC-P, IP | Rabbit IgG |
| VS3-WK1606 | Mouse Anti-MAPK9 Recombinant Antibody (clone 4D2) | IHC | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0346MZ | Chicken Anti-MAPK9 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2160 | Hi-Affi™ Recombinant Rabbit Anti-MAPK9 Monoclonal Antibody (DS2160AB) | WB, IP, IHC-P, ICC, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1099 | AbPlus™ Anti-MAPK9 Magnetic Beads (VS-0724-YC1099) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY293 | Rabbit Anti-NHP MAPK9 Recombinant Antibody (VS-1024-XY293) | WB, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC702 | CytoStream™ Rabbit Anti-MAPK9 Recombinant Antibody (VS13-YC702) | WB, ICC, IF, IHC-P, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4247 | Anti-MAPK9 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4248 | Anti-Mouse MAPK9 Immunohistochemistry Kit | IHC |
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