Mouse Anti-MCAM Recombinant Antibody (clone AA98) (CAT#: HPAB-0031-YJ)

Figure 1 Inhibiting CD146 improves the effects of radiation on suppressing SiHa cell survival.

Cell survival was detected by colony formation assay (n=3) and indicated as SF (surviving fraction). Control, SiHa cells without AA98 treatment. AA98, SiHa cells with AA98 treatment. * P<0.05.

Cheng, H. (2016). Inhibiting CD146 by its monoclonal antibody AA98 improves radiosensitivity of cervical cancer cells. Medical science monitor: international medical journal of experimental and clinical research, 22, 3328.

Figure 2 Inhibiting CD146 promotes caspase 3 and inhibits Bcl-XL

Protein expression compared to untreated cells. Control, SiHa cells without AA98 treatment. AA98, SiHa cells with AA98 treatment. Histogram shows the relative expression level of caspase 3 and Bcl-XL by comparing the grey-scale value to β-actin bands (n=3). *** P<0.001. Bcl-XL, B-cell lymphoma (extra-large).

Cheng, H. (2016). Inhibiting CD146 by its monoclonal antibody AA98 improves radiosensitivity of cervical cancer cells. Medical science monitor: international medical journal of experimental and clinical research, 22, 3328.

Figure 3 Flow cytometry analysis of AA98 antigen expression on HUVECs.

(Ai) AA98 antigen is not detectable on primary HUVECs (pHUVEC) immediately after isolation. (Aii) mAb AA98 recognizes its antigen on the stimulated HUVECs (sHUVEC) cultured in conditioned medium from hepatocarcinoma SMMC 7721 cells. (Aiii) An increase in mAb AA98 immunoreactivity is detected on primary HUVECs cultured in the presence of 10% FBS and is decreased after shifting the cells to serum-free medium for at least 12 hours.

Yan, X., Lin, Y., Yang, D., Shen, Y., Yuan, M., Zhang, Z., ... & Liu, Q. (2003). A novel anti-CD146 monoclonal antibody, AA98, inhibits angiogenesis and tumor growth. Blood, 102(1), 184-191.

Figure 4 Immunohistochemical analysis of the AA98 antigen on frozen sections from human tumor and normal tissues.

AA98 antigen is readily detectable in tumor neovasculature of hepatocarcinoma (Bii), thyroid tumor (Biv), and brain tumor (Bvi), whereas there was no significant mAb AA98 staining in corresponding normal tissue, such as liver (Bi), thyroid (Biii), and brain (Bv). Original magnification, × 200.

Yan, X., Lin, Y., Yang, D., Shen, Y., Yuan, M., Zhang, Z., ... & Liu, Q. (2003). A novel anti-CD146 monoclonal antibody, AA98, inhibits angiogenesis and tumor growth. Blood, 102(1), 184-191.

Figure 5 Characterization, purification, and identification of the AA98 antigen as CD146.

Western blot analysis of cell surface—biotinylated proteins of HUVECs immunoprecipitated by mAb AA98, separated under nonreducing or reducing conditions by SDS-PAGE, and detected by either HRP-conjugated streptavidin or mAb AA98 followed by HRP-conjugated anti—mouse IgG.

Yan, X., Lin, Y., Yang, D., Shen, Y., Yuan, M., Zhang, Z., ... & Liu, Q. (2003). A novel anti-CD146 monoclonal antibody, AA98, inhibits angiogenesis and tumor growth. Blood, 102(1), 184-191.

Figure 1 Mouse Anti-MCAM Recombinant Antibody (HPAB-0031-YJ) in ELISA

ELISA analysis of HPAB-0031-YJ was performed by coating with Human MCAM protein.
The secondary antibody: HRP-Anti-Mouse IgG (H+L)

Figure 2 Mouse Anti-MCAM Recombinant Antibody (HPAB-0031-YJ) in WB

WB analysis of HPAB-0031-YJ was performed with Human MCAM protein.

Lane1: Reducing antigen (0.1μg)
Lane2: Reducing antigen (0.3μg)
Lane3: Reducing antigen (0.6μg)