Rabbit Anti-MMP2 Recombinant Antibody (VS3-CJ494)
CAT#: VS3-CJ494
This product is a rabbit antibody that recognizes human, mouse, and rat MMP2.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1939/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human stomach
Immunohistochemical staining of human stomach shows moderate cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1939/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human endometrium
Immunohistochemical staining of human endometrium shows moderate cytoplasmic positivity in stromal cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2788/6952_B_5_3_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2788/6952_B_9_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2788/6952_A_9_3.jpg
Normal Tissue
Figure 6 Liver
Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1939/7518_A_9_4.jpg
Normal Tissue
Figure 7 Gallbladder
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1939/7518_A_4_4.jpg
Normal Tissue
Figure 8 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2788/6952_A_8_5.jpg
Normal Tissue
Figure 9 Testis
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2788/6952_A_6_6.jpg
Normal Tissue
Figure 10 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1939/7518_A_8_8.jpg
RNA Expression
Figure 11 RNA cell line category: Cell line enhanced (ASC diff, ASC TERT1, hTERT-RPE1, U-87 MG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000087245-MMP2
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Specifications
- Immunogen
- Recombinant protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat MMP2
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, IHC
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 40% Glycerol, 1% BSA, TBS, pH7.4.
- Preservative
- 0.05% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Western Blot, Immunohistochemistry.
Target
- Alternative Names
- CLG4; MONA; CLG4A; MMP-2; TBE-1; MMP-II
- Gene ID
- 4313
- UniProt ID
- P08253
- Sequence Similarities
- Belongs to the peptidase M10A family.
- Cellular Localization
- Cytoplasm, Extracellular matrix, Membrane, Mitochondrion, Nucleus, Secreted
- Post Translation Modifications
- Phosphorylation on multiple sites modulates enzymatic activity. Phosphorylated by PKC in vitro.
The propeptide is processed by MMP14 (MT-MMP1) and MMP16 (MT-MMP3). Autocatalytic cleavage in the C-terminal produces the anti-angiogenic peptide, PEX. This processing appears to be facilitated by binding integrinv/beta3.
- Protein Refseq
- NP_001121363.1; NP_001289437.1; NP_001289438.1
- Function
- Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues in association with MMP14.
PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels.
Isoform 2: Mediates the proteolysis of CHUK/IKKA and initiates a primary innate immune response by inducing mitochondrial-nuclear stress signaling with activation of the pro-inflammatory NF-kappaB, NFAT and IRF transcriptional pathways.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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| TAB-0566CL-F(E) | Mouse Anti-MMP2 Recombinant Antibody; Fab Fragment (TAB-0566CL-F(E)) | Inhib | Mouse Fab |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| NEUT-1747CQ | Recombinant Mouse Anti-MMP2 Antibody (CA719E3C) | Inhib, IHC, WB, ICC | IgG1 |
| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1355 | Anti-MMP2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4452 | Anti-Mouse MMP2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4451 | Anti-Human MMP2 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0625-YC226 | Recombinant Anti-MMP2 Eliminating Antibody, pH-Sensitive (3G11 var.) | Antigen-Sweeping In Vivo. | Human IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0825-YC255 | SmartAb™ Recombinant Anti-MMP2 pH-dependent Antibody (Clone 3G11) | ELISA | Human IgG1 kappa |
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