Mouse Anti-MTDH Monoclonal Antibody (clone 2F11C3) (CAT#: VS7-0425-WR380)
This product is a mouse IgG1 antibody that binds to human MTDH. The antibody 2F11C3 can be used for WB, IHC, and FC assays.
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to endoplasmic reticulum.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10932/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to endoplasmic reticulum.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10932/if_selected.jpg
Figure 2 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68204/1894_H7_3_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68204/1894_H7_3_selected.jpg
Figure 3 IHC staining of human small intestine
(Immunohistochemical staining of human small intestine shows cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10932/158178_A_4_2_selected.jpg
(Immunohistochemical staining of human small intestine shows cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10932/158178_A_4_2_selected.jpg
Figure 4 IHC staining of human cerebral corte
(Immunohistochemical staining of human cerebral cortex shows strong positivity.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68204/ihc_selected.jpg
(Immunohistochemical staining of human cerebral cortex shows strong positivity.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68204/ihc_selected.jpg
Figure 5 IHC staining of human smooth muscle
(Immunohistochemical staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68205/ihc_selected.jpg
(Immunohistochemical staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68205/ihc_selected.jpg
Figure 6 Colon
(Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10932/158178_A_7_3.jpg
(Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10932/158178_A_7_3.jpg
Figure 7 Liver
(Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10932/158178_A_7_4.jpg
(Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10932/158178_A_7_4.jpg
Figure 8 Kidney
(Cells in glomeruli
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10932/158178_A_7_5.jpg
(Cells in glomeruli
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10932/158178_A_7_5.jpg
Figure 9 Testis
(Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10932/158178_A_6_6.jpg
(Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10932/158178_A_6_6.jpg
Figure 10 Lymph node
(Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10932/158178_A_8_8.jpg
(Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10932/158178_A_8_8.jpg
Figure 11 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000147649-MTDH
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000147649-MTDH
Specifications
- Immunogen
- Recombinant human MTDH fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human MTDH
- Species Reactivity
- Human
- Clone
- 2F11C3
- Molecule Class
- Metadherin
- Applications
- WB, IHC, FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- MTDH; metadherin; 3D3; AEG1; AEG-1; LYRIC; LYRIC/3D3; protein LYRIC; 3D3/LYRIC; astrocyte elevated gene 1; metastasis adhesion protein; astrocyte elevated gene-1 protein; lysine-rich CEACAM1 co-isolated protein
- Gene ID
- 92140
- UniProt ID
- A0A024R9D2
- Long Name
- Metadherin
- Cellular Localization
- Cell junction; Cytoplasm; Endoplasmic reticulum; Membrane; Nucleus; Tight junction
- Post Translation Modifications
- Acetylation; Phosphoprotein
- Protein Refseq
- NP_001350065.1
- Function
- Down-regulates SLC1A2/EAAT2 promoter activity when expressed ectopically. Activates the nuclear factor kappa-B (NF-kappa-B) transcription factor. Promotes anchorage-independent growth of immortalized melanocytes and astrocytes which is a key component in tumor cell expansion. Promotes lung metastasis and also has an effect on bone and brain metastasis, possibly by enhancing the seeding of tumor cells to the target organ endothelium. Induces chemoresistance.
Recommended Products
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-678 | Recombinant Anti-human MTDH Antibody | ELISA, WB, IF, FuncS | IgG |
| MHH-678 | Recombinant Human Anti-human MTDH Antibody | WB, IP, FuncS | IgG |
| VS3-FY969 | Recombinant Mouse Anti-MTDH Antibody (clone 1F3A3) | ELISA, WB, IHC, ICC, FC | Mouse IgG1 |
| VS3-FY970 | Recombinant Mouse Anti-MTDH Antibody (clone 8A4B12) | ELISA, WB, IHC, FC | Mouse IgG1 |
| VS7-0425-WR432 | Mouse Anti-MTDH Monoclonal Antibody (VS7-0425-WR432) | WB, IHC-P, IF, FC, ELISA | Mouse IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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