Mouse Anti-MTDH Monoclonal Antibody (clone 2F11C3)
CAT#: VS7-0425-WR380
This product is a mouse IgG1 antibody that binds to human MTDH. The antibody 2F11C3 can be used for WB, IHC, and FC assays.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to endoplasmic reticulum.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10932/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68204/1894_H7_3_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human small intestine
Immunohistochemical staining of human small intestine shows cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10932/158178_A_4_2_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IHC staining of human cerebral corte
Immunohistochemical staining of human cerebral cortex shows strong positivity.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68204/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 5 IHC staining of human smooth muscle
Immunohistochemical staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68205/ihc_selected.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10932/158178_A_7_3.jpg
Normal Tissue
Figure 7 Liver
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10932/158178_A_7_4.jpg
Normal Tissue
Figure 8 Kidney
Cells in glomeruli
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10932/158178_A_7_5.jpg
Normal Tissue
Figure 9 Testis
Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10932/158178_A_6_6.jpg
Normal Tissue
Figure 10 Lymph node
Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10932/158178_A_8_8.jpg
RNA Expression
Figure 11 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000147649-MTDH
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Specifications
- Immunogen
- Recombinant human MTDH fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human MTDH
- Species Reactivity
- Human
- Clone
- 2F11C3
- Molecule Class
- Metadherin
- Applications
- WB, IHC, FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- MTDH; metadherin; 3D3; AEG1; AEG-1; LYRIC; LYRIC/3D3; protein LYRIC; 3D3/LYRIC; astrocyte elevated gene 1; metastasis adhesion protein; astrocyte elevated gene-1 protein; lysine-rich CEACAM1 co-isolated protein
- Gene ID
- 92140
- UniProt ID
- A0A024R9D2
- Long Name
- Metadherin
- Cellular Localization
- Cell junction; Cytoplasm; Endoplasmic reticulum; Membrane; Nucleus; Tight junction
- Post Translation Modifications
- Acetylation; Phosphoprotein
- Protein Refseq
- NP_001350065.1
- Function
- Down-regulates SLC1A2/EAAT2 promoter activity when expressed ectopically. Activates the nuclear factor kappa-B (NF-kappa-B) transcription factor. Promotes anchorage-independent growth of immortalized melanocytes and astrocytes which is a key component in tumor cell expansion. Promotes lung metastasis and also has an effect on bone and brain metastasis, possibly by enhancing the seeding of tumor cells to the target organ endothelium. Induces chemoresistance.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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