Mouse Anti-MTDH Monoclonal Antibody (VS7-0425-WR432)

CAT#: VS7-0425-WR432

This product is a mouse IgG antibody that binds to human MTDH. The antibody can be used for WB, IHC-P, IF, FC, and ELISA assays.

Gene Expression
Figure 1 IF staining of human cell line A-431 Figure 2 IF staining of human cell line U-2 OS Figure 3 IHC staining of human small intestine Figure 4 IHC staining of human cerebral corte Figure 5 IHC staining of human smooth muscle Figure 6 Colon Figure 7 Liver Figure 8 Kidney Figure 9 Testis Figure 10 Lymph node Figure 11 RNA cell line category: Low cell line specificity

Specifications

  • Immunogen
  • Recombinant human MTDH fragment expressed in E. Coli.
  • Host Species
  • Mouse
  • Type
  • Mouse IgG
  • Specificity
  • Human MTDH
  • Species Reactivity
  • Human
  • Molecule Class
  • Metadherin
  • Applications
  • WB, IHC-P, IF, FC, ELISA
  • Conjugate
  • Unconjugated

Product Property

  • Clonality
  • Monoclonal
  • Purity
  • >95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid
  • Format
  • Liquid
  • Concentration
  • 1 mg/ml
  • Preservative
  • 0.03% sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
  • Shipping
  • Ice packs

Applications

  • Application Notes
  • Western Blot: 1:500 - 1:2000
    Flow Cytometry: 1:200 - 1:400
    Immunohistochemistry: 1:200 - 1:1000
    Enzyme-linked Immunosorbent Assay: 1:10000

Target

  • Alternative Names
  • MTDH; metadherin; 3D3; AEG1; AEG-1; LYRIC; LYRIC/3D3; protein LYRIC; 3D3/LYRIC; astrocyte elevated gene 1; metastasis adhesion protein; astrocyte elevated gene-1 protein; lysine-rich CEACAM1 co-isolated protein
  • Long Name
  • Metadherin
  • Cellular Localization
  • Cell junction; Cytoplasm; Endoplasmic reticulum; Membrane; Nucleus; Tight junction
  • Post Translation Modifications
  • Acetylation; Phosphoprotein
  • Function
  • Down-regulates SLC1A2/EAAT2 promoter activity when expressed ectopically. Activates the nuclear factor kappa-B (NF-kappa-B) transcription factor. Promotes anchorage-independent growth of immortalized melanocytes and astrocytes which is a key component in tumor cell expansion. Promotes lung metastasis and also has an effect on bone and brain metastasis, possibly by enhancing the seeding of tumor cells to the target organ endothelium. Induces chemoresistance.
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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

MSDS

COA

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Protocol & Troubleshooting

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

Isotype Control

Secondary Antibody

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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