Rabbit Anti-MUC5AC Recombinant Antibody (clone R05-4H2)

CAT#: VS3-XY3439

This product is a recombinant rabbit antibody targeting the human MUC5AC (Mucin 5AC, oligomeric mucus/gel-forming). It recognizes and binds to human MUC5AC. It has been determined using Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry or Immunofluorescence. The recommended dilutions of Western Blot, Immunohistochemistry-Paraffin and Immunofluorescence is 1/500-1/1000, 1/50-1/100 and 1/50-1/200, respectively.

Gene Expression
Figure 1 High expression in stomach 1 Figure 2 Low expression in liver Figure 3 Stomach Figure 4 RNA cell line category: Group enriched (A549, OE19)

Specifications

  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human MUC5AC
  • Species Reactivity
  • Human
  • Clone
  • R05-4H2
  • Applications
  • WB, IHC-P, ICC, IF
  • Related Disease
  • Dry Eye Syndrome; Biliary Tract Disease

Product Property

  • Clonality
  • Monoclonal
  • Aggregation
  • < 5%
  • Format
  • Liquid
  • Buffer
  • PBS (pH 7.4), 150 mM NaCl and 50% glycerol
  • Preservative
  • 0.02% sodium azide
  • Storage
  • Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
  • Shipping
  • Ice packs

Applications

  • Application Notes
  • WB: 1/500-1/1000
    IHC: 1/50-1/100
    IF: 1/50-1/200

Target

  • Alternative Names
  • TBM; leB; MUC5; mucin
  • Long Name
  • Mucin 5AC, oligomeric mucus/gel-forming
  • Cellular Localization
  • Secreted
  • Post Translation Modifications
  • Glycosylation at Asn205, Asn258, Asn415, Asn524, Asn1308, Trp1389, Trp1584, Trp1749, Trp1957, Trp2122, Thr2395, Thr2405, Thr2451, Thr2461, Thr2531, Thr2541, Thr2571, Thr2581, Thr2699, Thr2709, Thr2883, Thr2893, Thr2979, Thr2989, Thr3067, Thr3077, Trp3228, Trp3526, Asn3774, Trp3959, Thr4224, Thr4234, Thr4296, Thr4306, Thr4320, Thr4330, Thr4376, Thr4386, Thr4440, Thr4450, Thr4480, Thr4490, Thr4512, Thr4522, Thr4568, Thr4578, Trp4633, Asn4869, Asn4942, Asn5057, Asn5093, Asn5236, Asn5347, Asn5377, Asn5386, Asn5455, Asn5528, and Asn5591 (NX_P98088 [NX_P98088-1])
    Glycosylation from GlyConnect MUC5A_HUMAN (375)
    Glycosylation from GlyGen (P98088) 63 sites, 17 O-linked glycans (1 site)
  • Function
  • Gel-forming glycoprotein of gastric and respiratory tract epithelia that protects the mucosa from infection and chemical damage by binding to inhaled microorganisms and particles that are subsequently removed by the mucociliary system.
    Interacts with H.pylori in the gastric epithelium, Barrett's esophagus as well as in gastric metaplasia of the duodenum (GMD).
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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

MSDS

COA

Certificate of Analysis Lookup

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Protocol & Troubleshooting

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

Isotype Control

Recommended Dilution Buffer

Secondary Antibody

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