Mouse Anti-N. meningitidis porA Recombinant Antibody (clone 151,F-9) (CAT#: FAMAB-0238JF)
Recombinant monoclonal antibody specially binds to Neisseria meningitidis PorA P1.16. This mouse-human anti-p1.7 antibody (151,F-9) can be useful to study the protective effects against meningococcal disease.
Specific Inquiry
FC
Figure 1 Binding of polyclonal antiGNA33 antisera and control mAbs to live encapsulated NmB strains 2996, M3735, M4207, and MC58 as determined by indirect fluorescence flow cytometry. T
The control mAbs and antisera include an anti-serogroup B capsularspecific murine mAb (SEAM 12).
Granoff, D. M., Moe, G. R., Giuliani, M. M., Adu-Bobie, J., Santini, L., Brunelli, B., ... & Bracci, L. (2001). A novel mimetic antigen eliciting protective antibody to Neisseria meningitidis. The Journal of Immunology, 167(11), 6487-6496.
WB
Figure 2 Western blots of rGNA33 and total proteins prepared from E. coli or N. meningitidis strains using antiPorA P1.2 as the primary Ab.
The standard deviation across 5 replicates.
Granoff, D. M., Moe, G. R., Giuliani, M. M., Adu-Bobie, J., Santini, L., Brunelli, B., ... & Bracci, L. (2001). A novel mimetic antigen eliciting protective antibody to Neisseria meningitidis. The Journal of Immunology, 167(11), 6487-6496.
ELISA
Figure 3 Escape of Neisseria meningitidis strain 8047 from the PorA MAb P1.2- mediated serum bactericidal activity.
All results are representative of at least four independent experiments.
Tauseef, I., Ali, Y. M., & Bayliss, C. D. (2013). Phase variation of PorA, a major outer membrane protein, mediates escape of bactericidal antibodies by Neisseria meningitidis. Infection and immunity, 81(4), 1374-1380.
IHC
Figure 4 Immunohistochemical staining of inflammatory cells and the vascular endothelium in skin biopsy samples from patients with meningococcal disease.
Arrows indicate positive immunoperoxidase staining (brown) with the appropriate specific mouse monoclonal antibody (nuclei were counterstained with hematoxylin).
Harrison, O. B., Robertson, B. D., Faust, S. N., Jepson, M. A., Goldin, R. D., Levin, M., & Heyderman, R. S. (2002). Analysis of pathogen-host cell interactions in purpura fulminans: expression of capsule, type IV pili, and PorA by Neisseria meningitidis in vivo. Infection and immunity, 70(9), 5193-5201.
Specifications
- Immunogen
- Deoxycholate-extracted OMVs from strain 44/76
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Neisseria meningitidis PorA P1.16
- Species Reactivity
- N. meningitidis
- Clone
- 151,F-9
- Applications
- WB, ELISA, IHC, FC
- Related Disease
- Meningitis
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The N. meningitidis porA antibody has been reported in applications of Western Blot, Enzyme-linked Immunosorbent Assay, Immunohistochemistry, Flow Cytometry.
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0234JF | Mouse Anti-N. meningitidis porA Recombinant Antibody (clone 208,D-5) | WB, ELISA, IHC | Mouse IgG |
| FAMAB-0235JF | Human Anti-N. meningitidis porA Recombinant Antibody (clone 208,D-5-chIgG1) | FC, ELISA, FuncS | Chimeric (mouse/human) IgG1 |
| FAMAB-0239JF | Human Anti-N. meningitidis porA Recombinant Antibody (clone 151,F-9-chIgG1) | FC, ELISA, FuncS | Chimeric (mouse/human) IgG1 |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0234JF-F(E) | Mouse Anti-N. meningitidis porA Recombinant Antibody (clone 208,D-5); Fab Fragment | FC, ELISA, FuncS | Mouse Fab |
| FAMAB-0235JF-F(E) | Human Anti-N. meningitidis porA Recombinant Antibody (clone 208,D-5-chIgG1); Fab Fragment | FC, ELISA, FuncS | Human Fab |
| FAMAB-0238JF-F(E) | Mouse Anti-N. meningitidis porA Recombinant Antibody (clone 151,F-9); Fab Fragment | FC, ELISA, FuncS | Mouse Fab |
| FAMAB-0239JF-F(E) | Human Anti-N. meningitidis porA Recombinant Antibody (clone 151,F-9-chIgG1); Fab Fragment | FC, ELISA, FuncS | Human Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0234JF-S(P) | Mouse Anti-N. meningitidis porA Recombinant Antibody (clone 208,D-5); scFv Fragment | FC, ELISA, FuncS | Mouse scFv |
| FAMAB-0238JF-S(P) | Mouse Anti-N. meningitidis porA Recombinant Antibody (clone 151,F-9); scFv Fragment | FC, ELISA, FuncS | Mouse scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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