Rabbit Anti-NEDD8 Recombinant Antibody (clone R02-4G8) (CAT#: VS3-FY1635)
The R02-4G8 recombinant monoclonal antibody has the ability to specifically bind to human NEDD8. It is suitable for use in a variety of assays, including WB, IHC-F, IHC-P, ICC/IF, IP. We confirm the QC result of the antibody by western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/50-1/100), immunofluorescence (dilution 1/50-1/200), immunoprecipitation (dilution 1/20). Applications Tested: The NEDD8 antibody has been tested by western blot analysis of NEDD8 in Jurkat lysates.
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WB
Figure 1 Anti-NEDD8 Antibody (VS3-FY1635) in WB.
The NEDD8 expression in Jurkat lysates were detected using the anti-NEDD8 by western blot assay.
Specifications
- Immunogen
- Synthetic peptide of human NEDD8
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human NEDD8
- Species Reactivity
- Human
- Clone
- R02-4G8
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 9 kDa, while the observed molecular weight stands at 9 kDa.
- Related Disease
- Malignant Astrocytoma and Machado-Joseph Disease
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- Neural Precursor Cell Expressed, Developmentally Down-Regulated 8; Ubiquitin-Like Protein Nedd8; Neddylin; NEDD-8; Neural Precursor Cell Expressed Developmentally Down-Regulated Protein 8
- Gene ID
- 4738
- UniProt ID
- Q15843
- Sequence Similarities
- Belongs to the ubiquitin family.
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Cleavage of precursor form by UCHL3 or SENP8 is necessary for function.4 Publications, (Microbial infection) Deamidated at Gln-40 by bacterial cyclomodulin Cif produced by enteropathogenic E.coli, Y.pseudotuberculosis or B.pseudomallei, leading to impair NEDD8 ability to activate cullin-RING-based E3 ubiquitin-protein ligase complexes (CRL complexes) (PubMed:20688984, PubMed:21903097, PubMed:23589306, PubMed:26632597, PubMed:23175788)., Deamidation occurs on NEDD8-modified cullins (PubMed:20850415, PubMed:21903097)., NEDD8 deamidation prevents switching from the inactive to active state by maintaining the 'closed' structure of the CRL complexes (PubMed:23589306, PubMed:26632597)., Deamidation may also impair its deconjugation by the COP9 signalosome; However this result needs additional evidences (PubMed:20850415, PubMed:21903097).
- Protein Refseq
- NP_006147.1
- Function
- Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis via its conjugation to a limited number of cellular proteins, such as cullins or p53/TP53.
Attachment of NEDD8 to cullins is critical for the recruitment of E2 to the cullin-RING-based E3 ubiquitin-protein ligase complex, thus facilitating polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.
Attachment of NEDD8 to p53/TP53 inhibits p53/TP53 transcriptional activity.
Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-945-VHH | Recombinant Anti-human NEDD8 VHH Single Domain Antibody | IHC, ICC, IP, ChiP, Neut | Llama VHH |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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