Rabbit Anti-NEDD8 Recombinant Antibody (clone R02-4G8) (CAT#: VS3-FY1635)
The R02-4G8 recombinant monoclonal antibody has the ability to specifically bind to human NEDD8. It is suitable for use in a variety of assays, including WB, IHC-F, IHC-P, ICC/IF, IP. We confirm the QC result of the antibody by western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/50-1/100), immunofluorescence (dilution 1/50-1/200), immunoprecipitation (dilution 1/20). Applications Tested: The NEDD8 antibody has been tested by western blot analysis of NEDD8 in Jurkat lysates.
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WB
Figure 1 Anti-NEDD8 Antibody (VS3-FY1635) in WB.
The NEDD8 expression in Jurkat lysates were detected using the anti-NEDD8 by western blot assay.
Specifications
- Immunogen
- Synthetic peptide of human NEDD8
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human NEDD8
- Species Reactivity
- Human
- Clone
- R02-4G8
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 9 kDa, while the observed molecular weight stands at 9 kDa.
- Related Disease
- Malignant Astrocytoma and Machado-Joseph Disease
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- Neural Precursor Cell Expressed, Developmentally Down-Regulated 8; Ubiquitin-Like Protein Nedd8; Neddylin; NEDD-8; Neural Precursor Cell Expressed Developmentally Down-Regulated Protein 8
- Gene ID
- 4738
- UniProt ID
- Q15843
- Sequence Similarities
- Belongs to the ubiquitin family.
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Cleavage of precursor form by UCHL3 or SENP8 is necessary for function.4 Publications, (Microbial infection) Deamidated at Gln-40 by bacterial cyclomodulin Cif produced by enteropathogenic E.coli, Y.pseudotuberculosis or B.pseudomallei, leading to impair NEDD8 ability to activate cullin-RING-based E3 ubiquitin-protein ligase complexes (CRL complexes) (PubMed:20688984, PubMed:21903097, PubMed:23589306, PubMed:26632597, PubMed:23175788)., Deamidation occurs on NEDD8-modified cullins (PubMed:20850415, PubMed:21903097)., NEDD8 deamidation prevents switching from the inactive to active state by maintaining the 'closed' structure of the CRL complexes (PubMed:23589306, PubMed:26632597)., Deamidation may also impair its deconjugation by the COP9 signalosome; However this result needs additional evidences (PubMed:20850415, PubMed:21903097).
- Protein Refseq
- NP_006147.1
- Function
- Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis via its conjugation to a limited number of cellular proteins, such as cullins or p53/TP53.
Attachment of NEDD8 to cullins is critical for the recruitment of E2 to the cullin-RING-based E3 ubiquitin-protein ligase complex, thus facilitating polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.
Attachment of NEDD8 to p53/TP53 inhibits p53/TP53 transcriptional activity.
Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-945-VHH | Recombinant Anti-human NEDD8 VHH Single Domain Antibody | IHC, ICC, IP, ChiP, Neut | Llama VHH |
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