AibGenesis™ Rabbit Anti-NFYA Recombinant Antibody (AibG-Rb-FY273)
CAT#: VS14-YC476
Crafted via advanced AI modeling, the anti-NFYA antibody provides precise and high-affinity targeting of NFYA across multiple species. NFYA is the regulatory and DNA-binding subunit of the heterotrimeric NF-Y (Nuclear Factor Y) complex, which specifically recognizes the CCAAT box-one of the most common promoter elements in the human genome. It acts as a master "gatekeeper" for the cell cycle and metabolic programming by integrating signals from the p53, E2F, and TGF-beta signaling pathways. Because NFYA is the only subunit of the complex with sequence-specific DNA binding capacity, its expression and alternative splicing serve as the rate-limiting step for the assembly of the active transcriptional apparatus.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50779/835_D10_1_selected.jpg
Normal Tissue
Figure 2 IHC staining of human stomach, lower
Immunohistochemical staining of human stomach, Lower shows strong nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50779/ihc_selected.jpg
Normal Tissue
Figure 3 Bone marrow.
High expression in bone marrow
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9250/24621_B_6_4_val_selected.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9250/24621_A_7_8.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50779/123398_B_8_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50779/123398_A_7_3.jpg
Normal Tissue
Figure 7 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50779/123398_A_7_4.jpg
Normal Tissue
Figure 8 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50779/123398_A_8_5.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000001167-NFYA
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- NFYA
- Species Reactivity
- Human, Mouse, Rat
- Clone
- AibG-Rb-FY273
- Applications
- Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FC)
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity purified
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% Glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- Western Blot (WB): 1/500-1/2000
Immunocytochemistry (ICC): 1/50-1/200
Immunofluorescence (IF): 1/50-1/200
Immunoprecipitation (IP): 1/50
Flow Cytometry (FC): 1/50
Target
- Alternative Names
- Nuclear Transcription Factor Y Subunit Alpha; Nuclear Transcription Factor Y Subunit A; Nuclear Transcription Factor Y, Alpha; NF-YA; CCAAT-Binding Transcription Factor Subunit B; CAAT-Box DNA Binding Protein Subunit A; CAAT Box DNA-Binding Protein Subunit A;
- Gene ID
- 4800
- UniProt ID
- P23511
- Long Name
- Nuclear Transcription Factor Y Subunit Alpha
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "NFYA"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3146z | Mouse Anti-NFYA Recombinant Antibody (clone 42B8) | WB, IP, IF, IHC, ELISA | Mouse IgG2a, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2438 | Hi-Affi™ Recombinant Rabbit Anti-NFYA Monoclonal Antibody (DS2438AB) | WB, ICC, IP, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-FY1040 | Recombinant Rabbit Anti-NFYA Antibody (clone R01-5G1) | WB, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC744 | AbPlus™ Anti-NFYA Magnetic Beads (VS-0724-YC744) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC804 | Rabbit Anti-NFYA Recombinant Antibody (VS13-YC804) | WB, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0426-FY610 | AibGenesis™ Rabbit Anti-NFYA Recombinant Antibody (AibG-Rb-NF610) | WB, ICC, FC, IP | Rabbit IgG |
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