Rabbit Anti-PAK1 Recombinant Antibody (clone 4F10) (CAT#: ZG-0495U)
Rabbit Anti-PAK1 Antibody (clone 4F10) displayed high specificity against the PAK1 protein of Human and Mouse.
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Custom Production
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WB
Figure 1 Rabbit Anti-PAK1 Antibody (ZG-0495U) in WB
Western Blot
Positive WB detected in: NIH/3T3 whole cell lysate, MCF-7 whole cell lysate, ntera-2 whole cell lysate, Mouse brain tissue
All lanes: PAK1 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 61, 62 kDa
Observed band size: 61 kDa
IF
Figure 2 Rabbit Anti-PAK1 Antibody (ZG-0495U) in IF
Immunofluorescence staining of Hela Cells with ZG-0495U at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).
Specifications
- Immunogen
- A synthesized peptide derived from human PAK1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human PAK1
- Species Reactivity
- Human, Mouse
- Clone
- 4F10
- Applications
- ELISA, WB, IF
- Conjugate
- Unconjugated
- Related Disease
- ntellectual Developmental Disorder With Macrocephaly, Seizures, And Speech Delay and Gastroesophageal Junction Adenocarcinoma.
Product Property
- Purification
- Affinity chromatography purified
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunofluorescence. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IF:1:20-1:200
Target
- Alternative Names
- Serine/threonine-protein kinase PAK 1; EC 2.7.11.1; Alpha-PAK; p21-activated kinase 1; PAK-1; p65-PAK; PAK1
- Gene ID
- 5058
- UniProt ID
- Q13153
Related Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IHC Kits & Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2175z | Mouse Anti-PAK1 Recombinant Antibody (clone 3A7) | WB, ELISA, IHC | Mouse IgG1, κ |
| MOB-1535CT | Recombinant Mouse anti-Human PAK1 Monoclonal antibody (EML2308) | IHC-P, WB | |
| MRO-2355-CN | Recombinant Rabbit Anti-PAK1 (phosphorylated Ser144+Ser141+Ser139) Monoclonal Antibody (CBACN-644) | WB, IF, IHC, IP, FC | Rabbit IgG |
| VS3-CJ183 | Rabbit Anti-PAK1 Recombinant Antibody (VS3-CJ183) | WB, ICC, IF, IHC, IP, FC | Rabbit IgG |
| VS3-FY1090 | Recombinant Rabbit Anti-PAK1 Antibody (clone R02-5G3) | WB, IHC-Fr, IHC-P, ICC, IF, IP | Rabbit IgG |
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