Rabbit Anti-PAK1 Recombinant Antibody (clone 4F10) (CAT#: ZG-0495U)
Rabbit Anti-PAK1 Antibody (clone 4F10) displayed high specificity against the PAK1 protein of Human and Mouse.
Specific Inquiry
WB
Figure 1 Rabbit Anti-PAK1 Antibody (ZG-0495U) in WB
Western Blot
Positive WB detected in: NIH/3T3 whole cell lysate, MCF-7 whole cell lysate, ntera-2 whole cell lysate, Mouse brain tissue
All lanes: PAK1 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 61, 62 kDa
Observed band size: 61 kDa
IF
Figure 2 Rabbit Anti-PAK1 Antibody (ZG-0495U) in IF
Immunofluorescence staining of Hela Cells with ZG-0495U at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).
Specifications
- Immunogen
- A synthesized peptide derived from human PAK1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human PAK1
- Species Reactivity
- Human, Mouse
- Clone
- 4F10
- Applications
- ELISA, WB, IF
- Conjugate
- Unconjugated
- Related Disease
- ntellectual Developmental Disorder With Macrocephaly, Seizures, And Speech Delay and Gastroesophageal Junction Adenocarcinoma.
Product Property
- Purification
- Affinity chromatography purified
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunofluorescence. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IF:1:20-1:200
Target
- Alternative Names
- Serine/threonine-protein kinase PAK 1; EC 2.7.11.1; Alpha-PAK; p21-activated kinase 1; PAK-1; p65-PAK; PAK1
- Gene ID
- 5058
- UniProt ID
- Q13153
Related Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2175z | Mouse Anti-PAK1 Recombinant Antibody (clone 3A7) | WB, ELISA, IHC | Mouse IgG1, κ |
| MOB-1535CT | Recombinant Mouse anti-Human PAK1 Monoclonal antibody (EML2308) | IHC-P, WB | |
| MRO-2355-CN | Recombinant Rabbit Anti-PAK1 (phosphorylated Ser144+Ser141+Ser139) Monoclonal Antibody (CBACN-644) | WB, IF, IHC, IP, FC | Rabbit IgG |
| VS3-FY1090 | Recombinant Rabbit Anti-PAK1 Antibody (clone R02-5G3) | WB, IHC-Fr, IHC-P, ICC, IF, IP | Rabbit IgG |
| VS3-FY1091 | Recombinant Rabbit Anti-PAK1 Antibody (clone R08-5I5) | WB, ICC, IF, IP | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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