Recombinant Rabbit Anti-PAK1 Antibody (clone R02-5G3) (CAT#: VS3-FY1090)

This product is a recombinant rabbit antibody that recognizes PAK1. This antibody has been reported for use in Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation. The clone R02-5G3 is specific for human, mouse, rat, Hamster PAK1. The antigen is synthetic peptide of human pak1.


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IHC-P

Figure 1. Recombinant Rabbit Anti-PAK1 Antibody (clone R02-5G3) in IHC-P.

Figure 1. Recombinant Rabbit Anti-PAK1 Antibody (clone R02-5G3) in IHC-P.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using PAK1 antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.

WB

Figure 2. Recombinant Rabbit Anti-PAK1 Antibody (clone R02-5G3) in WB.

Figure 2. Recombinant Rabbit Anti-PAK1 Antibody (clone R02-5G3) in WB.

Western Blot analysis of PAK1 from Hela, CHO-K1, C6, Jurkat lysates using PAK1 Antibody.


Specifications

  • Immunogen
  • Synthetic peptide of human PAK1.
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human, Mouse, Rat, Hamster PAK1
  • Species Reactivity
  • Human, Mouse, Rat, Hamster
  • Clone
  • R02-5G3
  • Applications
  • Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
  • Conjugate
  • Unconjugated
  • MW
  • Calculated MW: 61 kDa; Observed MW: 61 kDa

Product Property

  • Purification
  • Affinity Purified
  • Purity
  • >95% as determined by SDS-PAGE
  • Buffer
  • 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
  • Preservative
  • 0.01% Sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Western Blot: 1/500-1/1000
    Immunohistochemistry-Paraffin: 1/50-1/100
    Immunofluorescence: 1/50-1/200
    Immunoprecipitation: 1/20

Target

  • Alternative Names
  • P21 (RAC1) Activated Kinase 1; P21/Cdc42/Rac1-Activated Kinase 1 (STE20 Homolog, Yeast); P21/Cdc42/Rac1-Activated Kinase 1 (Yeast Ste20-Related); P21 Protein (Cdc42/Rac)-Activated Kinase 1; STE20 Homolog, Yeast; EC 2.7.11.1; Alpha-PAK
  • Cellular Localization
  • Cell junction, Cell membrane, Cell projection, Chromosome, Cytoplasm, Cytoskeleton, Membrane, Nucleus

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Related Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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