Mouse Anti-pan Tau Recombinant Antibody (clone tau5)
CAT#: VS-0623-WK90
The antibody clone tau5 was produced by immunizing mouse with tau. It is a pan-tau antibody (epitope AA210-241). It can be used for detection of human tau in ELISA, WB, IHC and IF staining.
Published Data
IHC
Figure 1 Cell lysates and media transfected with iBs and scFvs were applied to paraffin-embedded sections of 10-month-old rTg4510 tau transgenic mice.
Cortical staining of neurofibrillary tangles was visualized with a biotinylated anti-c-myc secondary antibody. EGFP palys as a negative control.
ELISA
Figure 2 Cell lysates and media containing scFvs and iBs were subjected to direct ELISA.
Plates were coated with recombinant full-length tau, p-tau, or amyloid β as a control. Anti-c-myc HRP secondary antibody served as a detection.
FucnS
Figure 3 p-Tau-Specific iBs and scFvs Delay the Onset of Hindlimb Paralysis of Homozygous JNPL3 Mice.
JNPL3 mice for the survival cohort were intraspinally injected at P0 with pAAV2/8 (2 × 10e10 genomes) expressing PHF1, CP13, and Tau5 scFvs. Control mice were injected with rAAV-EGFP. All injected mice reached a terminal state (paralysis) within 12 months post-injection (n = 10-12). Median age of terminal state and relative increase of lifespan.
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Specifications
- Immunogen
- Tau
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human tau
- Species Reactivity
- Human
- Clone
- tau5
- Applications
- ELISA, WB, IHC, IF, FuncS
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No Preservative
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Immunohistochemical staining was done with cell lysates and media containing scFvs and iBs diluted 1/10 with PBS and applied to paraffin-fixed brain tissue slides from 10-month-old mice.
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHH-951-S(P) | Recombinant Human Anti-Tau Antibody scFv Fragment | ELISA, WB, FuncS | scFv |
| HPAB-0773LY-S(P) | Human Anti-Tau Recombinant Antibody; scFv Fragment (HPAB-0773LY-S(P)) | ELISA | Human scfv |
| HPAB-2561LY-S(P) | Human Anti-Tau Recombinant Antibody; scFv Fragment (HPAB-2561LY-S(P)) | WB, IHC, ELISA | Human scFv |
| HPAB-2562LY-S(P) | Human Anti-Tau Recombinant Antibody; scFv Fragment (HPAB-2562LY-S(P)) | WB, IHC, ELISA | Human scFv |
| HPAB-2563LY-S(P) | Human Anti-Tau Recombinant Antibody; scFv Fragment (HPAB-2563LY-S(P)) | WB, IHC, ELISA | Humanized scfv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0235MC | Anti-Human Tau Antibody (Acetyl K280) | ELISA, dot blot, WB | |
| MOB-0236MC | Anti-Human Tau Antibody (Acetyl K686) | ELISA, WB | |
| HPAB-0773LY | Human Anti-Tau Recombinant Antibody (HPAB-0773LY) | ELISA | Human IgG |
| FAMAB-0352WJ | Mouse Anti-Tau Recombinant Antibody (clone MHT2) | ELISA, WB, IHC | Mouse IgG |
| PABS-0209 | Mouse Anti-Tau Recombinant Antibody (PABS-0209) | ELISA, WB, DB, IHC | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0427CL | Human Anti-Tau Recombinant Antibody (TAB-0427CL) | ELISA, WB, IHC, FuncS | Human IgG |
| TAB-0428CL | Human Anti-Tau Recombinant Antibody (TAB-0428CL) | ELISA, WB | Human IgG |
| TAB-0429CL | Human Anti-Tau Recombinant Antibody (TAB-0429CL) | ELISA, WB | Human IgG |
| TAB-0427CL-S(P) | Human Anti-Tau Recombinant Antibody; scFv Fragment (TAB-0427CL-S(P)) | ELISA, WB, IHC, FuncS | Human scFv |
| TAB-0428CL-S(P) | Human Anti-Tau Recombinant Antibody; scFv Fragment (TAB-0428CL-S(P)) | ELISA, WB | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-527CT | Human Anti-Tau Recombinant Antibody (TAB-527CT) | IF, FuncS | Humanized antibody |
| TAB-533CT | Humanized Anti-Tau Recombinant Antibody (TAB-533CT) | WB, Inhib, IHC, FuncS, IF | Humanized IgG |
| TAB-527CT-S(P) | Human Anti-Tau Recombinant Antibody; scFv Fragment (TAB-527CT-S(P)) | WB, ELISA, IHC | Human scFv |
| TAB-533CT-S(P) | Humanized Anti-Tau Recombinant Antibody; scFv Fragment (TAB-533CT-S(P)) | WB, ELISA, IP, IHC, Neut | Humanized scFv |
| TAB-527CT-F(E) | Human Anti-Tau Recombinant Antibody; Fab Fragment (TAB-527CT-F(E)) | WB, ELISA, IHC | Humanized Fab |
| CAT | Product Name | Application | Type |
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| TAB-519CT-F(E) | Mouse Anti-Tau Recombinant Antibody; Fab Fragment (TAB-519CT-F(E)) | WB, ELISA | Mouse Fab |
| TAB-520CT-F(E) | Mouse Anti-Tau Recombinant Antibody; Fab Fragment (TAB-520CT-F(E)) | WB, ELISA | Mouse Fab |
| TAB-521CT-F(E) | Mouse Anti-Tau Recombinant Antibody; Fab Fragment (TAB-521CT-F(E)) | WB, ELISA | Mouse Fab |
| TAB-522CT-F(E) | Mouse Anti-Tau Recombinant Antibody; Fab Fragment (TAB-522CT-F(E)) | WB, ELISA | Mouse Fab |
| TAB-523CT-F(E) | Mouse Anti-Tau Recombinant Antibody; Fab Fragment (TAB-523CT-F(E)) | WB, ELISA | Mouse Fab |
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| VS-0525-YC177 | Recombinant Anti-Tau (Thr169/175 x AA 218-225) Biparatopic Antibody, Tandem scFv (Clone MHT2 x Clone Tau5) | ELISA | Tandem scFv |
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