Mouse Anti-PCK2 Recombinant Antibody (clone 3D3D9) (CAT#: VS7-0425-WR574)
This product is a mouse IgG1 antibody that binds to human PCK2. The antibody 3D3D9 recognizes specific amino acid domain of human PCK2 (AA: 44-175). It can be used for WB, IHC, ICC, and FC assays. It cross reacts with rat and monkey.
Specific Inquiry
Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line MCF7
(Immunofluorescent staining of human cell line MCF7 shows localization to mitochondria.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51162/if_selected.jpg
(Immunofluorescent staining of human cell line MCF7 shows localization to mitochondria.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51162/if_selected.jpg
Figure 2 IF staining of human cell line Hep G2
(Immunofluorescent staining of human cell line Hep G2 shows localization to mitochondria.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/53502/1334_B5_2_selected.jpg
(Immunofluorescent staining of human cell line Hep G2 shows localization to mitochondria.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/53502/1334_B5_2_selected.jpg
Figure 3 Duodenum
(High expression in duodenum)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51162/124859_A_7_2_selected.jpg
(High expression in duodenum)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51162/124859_A_7_2_selected.jpg
Figure 4 Small intestine
(Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Gradient Gradient: Ascending Enterocytes - Goblet cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51162/124859_A_4_2.jpg
(Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Gradient Gradient: Ascending Enterocytes - Goblet cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51162/124859_A_4_2.jpg
Figure 5 Colon
(Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Goblet cells Staining: High Intensity: Strong Quantity:>75% Mucosal lymphoid cells Staining: Medium Intensity: Moderate Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51162/124859_A_9_3.jpg
(Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Goblet cells Staining: High Intensity: Strong Quantity:>75% Mucosal lymphoid cells Staining: Medium Intensity: Moderate Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51162/124859_A_9_3.jpg
Figure 6 Liver
(Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51162/124859_A_9_4.jpg
(Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51162/124859_A_9_4.jpg
Figure 7 RNA cell line category: Cell line enhanced (HHSteC, hTCEpi, SK-MEL-30)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000100889-PCK2
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000100889-PCK2
Specifications
- Immunogen
- Recombinant human PCK2 (AA: 44-175) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human PCK2
- Species Reactivity
- Human, Rat, Monkey
- Clone
- 3D3D9
- Molecule Class
- Phosphoenolpyruvate Carboxykinase
- Applications
- WB, IHC, ICC, FC
- Conjugate
- Unconjugated
- Related Disease
- Mitochondrial phosphoenolpyruvate carboxykinase deficiency (M-PEPCKD)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Immunocytochemistry: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- PCK2; Phosphoenolpyruvate Carboxykinase [GTP], Mitochondrial; PEPCK; PEPCK-M; PE; PEP Carboxykinase; EC 4.1.1.32; Phosphoenolpyruvate Carboxykinase 2 (Mitochondrial); Phosphopyruvate Carboxylase
- Gene ID
- 5106
- UniProt ID
- Q16822
- Long Name
- Phosphoenolpyruvate Carboxykinase 2, Mitochondrial
- Cellular Localization
- Mitochondrion
- Post Translation Modifications
- Acetylation; Phosphoprotein
- Protein Refseq
- NP_001018083.2
- Function
- Decarboxylase; Lyase
Recommended Products
Isotype Control
Secondary Antibody
Reconstitution Buffer
Recommended Dilution Buffer
Related Resources
BCR Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1318CT | Recombinant Mouse anti-Human PCK2 Monoclonal antibody (2C9) | ELISA, WB | |
| MRO-1183-CN | Recombinant Rabbit Anti-PCK2 Monoclonal Antibody (JB52-39) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-WK316 | Rabbit Anti-PCK2 Recombinant Antibody (clone JB52-39) | WB, ELISA | Rabbit IgG |
| VS7-0425-WR573 | Mouse Anti-PCK2 Recombinant Antibody (clone 3D3E11) | WB, IHC, FC | Mouse IgG1 |
| VS7-0425-WR575 | Mouse Anti-PCK2 Recombinant Antibody (VS7-0425-WR575) | WB | Mouse IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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