Rabbit Anti-PCNA Recombinant Antibody (clone R04-3I2)
CAT#: VS3-FY1817
This R04-3I2 recombinant monoclonal antibody has the ability to specifically bind to human PCNA. It cross-reacts with mouse. This PCNA antibody has been teted positive by western blot analysis of PCNA in Hela lysates.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/148/if_selected.jpg
Normal Tissue
Figure 2 Colon
Glandular cells
Staining:
High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30523/64162_A_9_3.jpg
Normal Tissue
Figure 3 Testis
Leydig cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
Preleptotene spermatocytes
Staining:
Medium
Intensity: Strong
Quantity: <25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30523/64162_A_6_6.jpg
Normal Tissue
Figure 4 Bone marrow
Hematopoietic cells
Staining:
High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30523/64162_B_5_4.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining:
Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30523/64162_A_7_8.jpg
RNA Expression
Figure 6 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000132646-PCNA
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❯
WB
Figure 1 Anti-PCNA Antibody (VS3-FY1817) in WB.
The PCNA expression in Hela lysates were detected using the anti-PCNA by western blot assay.
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Specifications
- Immunogen
- Synthetic peptide of human PCNA
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse PCNA
- Species Reactivity
- Human, Mouse
- Clone
- R04-3I2
- Applications
- Western Blot
- MW
- The calculated weight of the target protein is approximately 29 kDa, while the observed molecular weight stands at 29 kDa.
- Related Disease
- Ataxia-Telangiectasia-Like Disorder 2 and Bowen's Disease
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/5000-1/10000
Target
- Alternative Names
- Proliferating Cell Nuclear Antigen; Cyclin; DNA Polymerase Delta Auxiliary Protein; ATLD2
- Gene ID
- 5111
- UniProt ID
- P12004
- Sequence Similarities
- Belongs to the PCNA family.
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Phosphorylated; Acetylated by CREBBP and p300/EP300; preferentially acetylated by CREBBP on Lys-80, Lys-13 and Lys-14 and on Lys-77 by p300/EP300 upon loading on chromatin in response to UV irradiation; Ubiquitinated
- Protein Refseq
- NP_002583.1; NP_872590.1
- Function
- Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| HPAB-0205-YC-F(E) | Mouse Anti-PCNA Recombinant Antibody (clone 12D10); Fab Fragment | ELISA, WB, IHC | Mouse Fab |
| HPAB-N0376-YC-F(E) | Mouse Anti-PCNA Recombinant Antibody; Fab Fragment (HPAB-N0376-YC-F(E)) | ELISA, FC, WB | Mouse Fab |
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