Mouse Anti-PCNA Recombinant Antibody (VS3-CJ610)
CAT#: VS3-CJ610
This product is a mouse antibody that recognizes human, mouse, and rat PCNA.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/148/if_selected.jpg
Normal Tissue
Figure 2 Colon
Glandular cells
Staining:
High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30523/64162_A_9_3.jpg
Normal Tissue
Figure 3 Testis
Leydig cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
Preleptotene spermatocytes
Staining:
Medium
Intensity: Strong
Quantity: <25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30523/64162_A_6_6.jpg
Normal Tissue
Figure 4 Bone marrow
Hematopoietic cells
Staining:
High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30523/64162_B_5_4.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining:
Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30523/64162_A_7_8.jpg
RNA Expression
Figure 6 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000132646-PCNA
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Specifications
- Immunogen
- Recombinant protein of human PCNA
- Host Species
- Mouse
- Type
- Mouse IgG1, κ
- Specificity
- Human, Mouse, Rat PCNA
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, IHC-P, FC
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A/G affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.3.
- Preservative
- 0.09% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Western Blot (1:3000), Immunohistochemistry-Parraffin (1:25), Flow Cytometry (1:25).
Target
- Alternative Names
- ATLD2
- Gene ID
- 5111
- UniProt ID
- P12004
- Sequence Similarities
- Belongs to the PCNA family.
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Phosphorylated. Phosphorylation at Tyr-211 by EGFR stabilizes chromatin-associated PCNA.
Acetylated by CREBBP and p300/EP300; preferentially acetylated by CREBBP on Lys-80, Lys-13 and Lys-14 and on Lys-77 by p300/EP300 upon loading on chromatin in response to UV irradiation (PubMed:24939902, PubMed:19419956).
Lysine acetylation disrupts association with chromatin, hence promoting PCNA ubiquitination and proteasomal degradation in response to UV damage in a CREBBP- and EP300-dependent manner (PubMed:24939902).
Acetylation disrupts interaction with NUDT15 and promotes degradation (PubMed:19419956).
Ubiquitinated (PubMed:24939902, PubMed:20227374).
Following DNA damage, can be either monoubiquitinated to stimulate direct bypass of DNA lesions by specialized DNA polymerases or polyubiquitinated to promote recombination-dependent DNA synthesis across DNA lesions by template switching mechanisms. Following induction of replication stress, monoubiquitinated by the UBE2B-RAD18 complex on Lys-164, leading to recruit translesion (TLS) polymerases, which are able to synthesize across DNA lesions in a potentially error-prone manner. An error-free pathway also exists and requires non-canonical polyubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases, HLTF, RNF8 and SHPRH. This error-free pathway, also known as template switching, employs recombination mechanisms to synthesize across the lesion, using as a template the undamaged, newly synthesized strand of the sister chromatid. Monoubiquitination at Lys-164 also takes place in undamaged proliferating cells, and is mediated by the DCX(DTL) complex, leading to enhance PCNA-dependent translesion DNA synthesis. Sumoylated during S phase.
Methylated on glutamate residues by ARMT1/C6orf211.
- Protein Refseq
- NP_002583.1; NP_872590.1
- Function
- Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'- 5' exonuclease and 3'-phosphodiesterase, but not apurinic- apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
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|---|---|---|---|
| MOR-2616 | Hi-Affi™ Recombinant Rabbit Anti-PCNA Monoclonal Antibody (DS2616AB) | FC, IHC-P, WB | IgG |
| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0205-YC-S(P) | Mouse Anti-PCNA Recombinant Antibody (clone 12D10); scFv Fragment | ELISA, WB, IHC | Mouse scFv |
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| CAT | Product Name | Application | Type |
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| HPAB-N0376-YC-F(E) | Mouse Anti-PCNA Recombinant Antibody; Fab Fragment (HPAB-N0376-YC-F(E)) | ELISA, FC, WB | Mouse Fab |
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