Mouse Anti-PDCD1LG2 Recombinant Antibody (clone 7F11D11)

Name: Mouse Anti-PDCD1LG2 Recombinant Antibody (clone 7F11D11)
SKU: ZG-0252U
Rating: 5 (1 reviews)

CAT#: ZG-0252U

Mouse anti-PDCD1LG2 antibody (clone 7F11D11) displayed high specificity against the PDCD1LG2 protein of Human.

Tested Data Gene Expression

Figure 1 Mouse Anti-PDCD1LG2 Antibody (ZG-0252U) in WB

Positive WB detected in: HepG2 whole cell lysate, Hela whole cell lysate, PC-3 whole cell lysate, A549 whole cell lysate. All lanes PD-L2 antibody at 1: 2000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 31,21 KDa
Observed band size: 45-50 KDa
Exposure time: 5min

IHC

Figure 2 Mouse Anti-PDCD1LG2 Antibody (ZG-0252U) in IHC

IHC image of ZG-0252U diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Figure 3 Mouse Anti-PDCD1LG2 Antibody (ZG-0252U) in IF

Immunofluorescence staining of HepG2 cells with ZG-0252U at 1:100, counter-stained with DAPI. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Mouse IgG (H+L).

Figure 4 Mouse Anti-PDCD1LG2 Antibody (ZG-0252U) in FC

Overlay histogram showing 293 cells transfected with PD-L2 stained with ZG-0252U (red line). The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (2µg/1*106cells) for 1 h at 4°C. The secondary antibody used was R-PE-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG2b (2µg/1*106cells) used under the same conditions. Acquisition of>10,000 events was performed.

Figure 5 Mouse Anti-PDCD1LG2 Antibody (ZG-0252U) in IP

Immunoprecipitating PD-L2 in Hela whole cell lysate Lane 1: Mouse control IgG in Hela whole cell lysate
Lane 2: ZG-0252U (2µl) + Hela whole cell lysate (500µg)
Lane 3: Hela whole cell lysate (20µg)
For western blotting, the blot was detected with ZG-0252U at 1:2000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:2000

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Subcellular Location and Protein Expression Figure 1 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13411/104_F6_1_selected.jpg

Subcellular Location and Protein Expression Figure 2 IHC staining of human pancreas

Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity with granular pattern in exocrine glandular cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13411/ihc_selected.jpg

Subcellular Location and Protein Expression Figure 3 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13411/200_H4_1_blue_red_green.jpg

Normal Tissue Figure 4 Cerebral cortex

Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13411/30825_B_9_5.jpg

Normal Tissue Figure 5 Colon

Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13411/30825_A_9_3.jpg

Normal Tissue Figure 6 Liver

Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13411/30825_A_8_4.jpg

Normal Tissue Figure 7 Pancreas

Exocrine glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Pancreatic endocrine cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13411/30825_A_2_3.jpg

Normal Tissue Figure 8 Kidney

Cells in tubules
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13411/30825_A_9_5.jpg

Normal Tissue Figure 9 Testis

Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13411/30825_A_6_6.jpg

Normal Tissue Figure 10 Lymph node

Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13411/30825_A_9_8.jpg

RNA Expression Figure 11 RNA cell line category: Cell line enhanced (BJ, BJ hTERT+, HDLM-2, TIME

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000197646-PDCD1LG2

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Specifications

Immunogen
Recombinant Human Programmed cell death 1 ligand 2 protein (21-118AA)

Host Species
Mouse

Type
Mouse IgG2b

Specificity
Human PDCD1LG2

Species Reactivity
Human

Clone
7F11D11

Applications
ELISA, WB, IHC, IF, FC, IP

Conjugate
Unconjugated

Related Disease
Cysticercosis and Central Nervous System Lymphoma.

Product Property

Purification
Protein A purified

Purity
>95% as determined by SDS-PAGE

Buffer
PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.

Preservative
0.03% Proclin 300

Storage
Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, Immunoprecipitation. Specific conditions should be optimized by the end user.
WB:1:5000-1:640000, IHC:1:50-1:200, IF:1:50-1:200, FC:1:50-1:200, IP:2µl-5µl

Target

Alternative Names
B7DC; Btdc; PDL2; CD273; PD-L2; PDCD1L2; bA574F11.2; programmed cell death 1 ligand 2; B7 dendritic cell molecule; B7-DC; PD-1-ligand 2; PDCD1 ligand 2; butyrophilin B7-DC; programmed death ligand 2

Gene ID
80380

UniProt ID
Q9BQ51

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - ZG-0252U.pdf

MSDS

MSDS - ZG-0252U.pdf

COA

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Protocol & Troubleshooting

Immunofluorescence (IF)

Immunohistochemistry (IHC)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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