Mouse Anti-PDCD1LG2 Recombinant Antibody (clone 7F11D11)
CAT#: ZG-0252U
Mouse anti-PDCD1LG2 antibody (clone 7F11D11) displayed high specificity against the PDCD1LG2 protein of Human.
Tested Data
Gene Expression
WB
Figure 1 Mouse Anti-PDCD1LG2 Antibody (ZG-0252U) in WB
Positive WB detected in: HepG2 whole cell lysate, Hela whole cell lysate, PC-3 whole cell lysate, A549 whole cell lysate. All lanes PD-L2 antibody at 1: 2000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 31,21 KDa
Observed band size: 45-50 KDa
Exposure time: 5min
IHC
Figure 2 Mouse Anti-PDCD1LG2 Antibody (ZG-0252U) in IHC
IHC image of ZG-0252U diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IF
Figure 3 Mouse Anti-PDCD1LG2 Antibody (ZG-0252U) in IF
Immunofluorescence staining of HepG2 cells with ZG-0252U at 1:100, counter-stained with DAPI. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Mouse IgG (H+L).
FC
Figure 4 Mouse Anti-PDCD1LG2 Antibody (ZG-0252U) in FC
Overlay histogram showing 293 cells transfected with PD-L2 stained with ZG-0252U (red line). The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (2µg/1*106cells) for 1 h at 4°C. The secondary antibody used was R-PE-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG2b (2µg/1*106cells) used under the same conditions. Acquisition of>10,000 events was performed.
IP
Figure 5 Mouse Anti-PDCD1LG2 Antibody (ZG-0252U) in IP
Immunoprecipitating PD-L2 in Hela whole cell lysate Lane 1: Mouse control IgG in Hela whole cell lysate
Lane 2: ZG-0252U (2µl) + Hela whole cell lysate (500µg)
Lane 3: Hela whole cell lysate (20µg)
For western blotting, the blot was detected with ZG-0252U at 1:2000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:2000
❮
❯
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13411/104_F6_1_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human pancreas
Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity with granular pattern in exocrine glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13411/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13411/200_H4_1_blue_red_green.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13411/30825_B_9_5.jpg
Normal Tissue
Figure 5 Colon
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13411/30825_A_9_3.jpg
Normal Tissue
Figure 6 Liver
Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13411/30825_A_8_4.jpg
Normal Tissue
Figure 7 Pancreas
Exocrine glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Pancreatic endocrine cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13411/30825_A_2_3.jpg
Normal Tissue
Figure 8 Kidney
Cells in tubules
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13411/30825_A_9_5.jpg
Normal Tissue
Figure 9 Testis
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13411/30825_A_6_6.jpg
Normal Tissue
Figure 10 Lymph node
Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13411/30825_A_9_8.jpg
RNA Expression
Figure 11 RNA cell line category: Cell line enhanced (BJ, BJ hTERT+, HDLM-2, TIME
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000197646-PDCD1LG2
❮
❯
Specifications
- Immunogen
- Recombinant Human Programmed cell death 1 ligand 2 protein (21-118AA)
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- Human PDCD1LG2
- Species Reactivity
- Human
- Clone
- 7F11D11
- Applications
- ELISA, WB, IHC, IF, FC, IP
- Conjugate
- Unconjugated
- Related Disease
- Cysticercosis and Central Nervous System Lymphoma.
Product Property
- Purification
- Protein A purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
- Preservative
- 0.03% Proclin 300
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, Immunoprecipitation. Specific conditions should be optimized by the end user.
WB:1:5000-1:640000, IHC:1:50-1:200, IF:1:50-1:200, FC:1:50-1:200, IP:2µl-5µl
Target
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for ZG-0252U. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "Pdcd1lg2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-993-VHH | Recombinant Anti-Mouse Pdcd1lg2 VHH Single Domain Antibody | WB, ICC, ChiP, FA, ELISA | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1012CL | Human Anti-PDCD1LG2 Recombinant Antibody (TAB-1012CL) | FC | Human IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1013CL | Mouse Anti-PDCD1LG2 Recombinant Antibody (TAB-1013CL) | ELISA | Mouse IgG |
| TAB-1014CL | Mouse Anti-PDCD1LG2 Recombinant Antibody (TAB-1014CL) | ELISA, FC | Mouse IgG |
| TAB-1013CL-S(P) | Mouse Anti-PDCD1LG2 Recombinant Antibody; scFv Fragment (TAB-1013CL-S(P)) | ELISA | Mouse scFv |
| TAB-1014CL-S(P) | Mouse Anti-PDCD1LG2 Recombinant Antibody; scFv Fragment (TAB-1014CL-S(P)) | ELISA, FC | Mouse scFv |
| TAB-1013CL-F(E) | Mouse Anti-PDCD1LG2 Recombinant Antibody; Fab Fragment (TAB-1013CL-F(E)) | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1012CL-S(P) | Human Anti-PDCD1LG2 Recombinant Antibody; scFv Fragment (TAB-1012CL-S(P)) | FC | Human scFv |
| TAB-1012CL-F(E) | Human Anti-PDCD1LG2 Recombinant Antibody; Fab Fragment (TAB-1012CL-F(E)) | FC | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1839CQ | Recombinant Mouse Anti-PDCD1LG2 Antibody (MIH18) | FC, IHC, IF, BL | IgG1, κ |
| NEUT-1840CQ | Recombinant Rat Anti-Pdcd1lg2 Antibody (MAB0877) | BL, FC, IP, WB | IgG2 |
| NEUT-1841CQ | Recombinant Rat Anti-Pdcd1lg2 Antibody (TY25) | IHC-P, FC, WB, IP, BL, IHC-Fr | IgG2a, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-4357 | Hi-Affi™ Recombinant Rabbit Anti-PDCD1LG2 Monoclonal Antibody (SI447DS) | FC | IgG |
| MOR-4358 | Hi-Affi™ Recombinant Rabbit Anti-PDCD1LG2 Monoclonal Antibody (SI448DS) | FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0314-FY-S(P) | Human Anti-PDCD1LG2 Recombinant Antibody (clone CBLFY19); scFv Fragment | IHC, ELISA | Humanized scFv |
| HPAB-2927LY-S(P) | Mouse Anti-PDCD1LG2 Recombinant Antibody; scFv Fragment (HPAB-2927LY-S(P)) | ELISA | Mouse scfv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0314-FY-F(E) | Human Anti-PDCD1LG2 Recombinant Antibody (clone CBLFY19); Fab Fragment | IHC, ELISA | Humanized Fab |
| HPAB-2927LY-F(E) | Mouse Anti-PDCD1LG2 Recombinant Antibody; Fab Fragment (HPAB-2927LY-F(E)) | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FN-036CQ | Recombinant Mouse Anti-PDCD1LG2 Antibody (ANC8D12) | FC, ELISA, Block | Mouse IgG2a, κ |
| HPAB-2927LY | Mouse Anti-PDCD1LG2 Recombinant Antibody (HPAB-2927LY) | ELISA | Mouse IgG |
| VS3-WK1128 | Mouse Anti-PDCD1LG2 Recombinant Antibody (clone 3.2) | IF, FC | Mouse IgG1, κ |
| VS3-WK1129 | Mouse Anti-PDCD1LG2 Recombinant Antibody (VS3-WK1129) | IHC-P, WB | Mouse IgG1, κ |
| VS-0723-WK228 | Rabbit Anti-PDCD1LG2 Recombinant Antibody (VS-0723-WK228) | ELISA, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY174 | CytoStream™ Mouse Anti-PDCD1LG2 Recombinant Antibody (clone 24F.10C12) | FC | Mouse IgG2a, kappa |
| VS13-YC878 | CytoStream™ Mouse Anti-PDCD1LG2 Recombinant Antibody (VS13-YC878) | WB, ICC, IF, FC, ELISA | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC279 | Recombinant Anti-PDCD1LG2 Vesicular Antibody, EV Displayed (VS-0425-YC279) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5255 | Anti-PDCD1LG2 Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: Neut, ELISA, IF, IP, FuncS, FC, IHC
Application: FC, IP, ELISA, Neut, FuncS, IF, ICC
-2.png)
Application: WB, ELISA, FC, IHC, IP
Application: ELISA, WB, Neut, Funcs, IHC, Inhib
Application: Neut, FC, IHC-Fr, IP, BA
-4.jpg)
Application: FC
Application: ELISA, Cyt, PP, Inhib
Application: ELISA, FuncS
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
