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Mouse Anti-PDE4B Recombinant Antibody (clone 1D12) (CAT#: VS-0224-XY99)

The product is a mouse monoclonal antibody (IgG1) which recognizes phosphodiesterase 4B of human, mouse and rat origin. It was purified by Immunogen affinity. It has been tested by Western blot, Flow cytometry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry and Immunohistochemistry-paraffin.


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Subcellular Location
Figure 1 IF staining of human cell line AF22
Figure 1 IF staining of human cell line AF22
(Immunofluorescent staining of human cell line AF22 shows localization to the Golgi apparatus.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3005/1786_F1_2_selected.jpg
Normal Tissue
Figure 2 IHC staining of human epididymis
Figure 2 IHC staining of human epididymis
(Immunohistochemical staining of human epididymis shows strong cytoplasmic positivity with granular pattern in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3005/ihc_selected.jpg
Figure 3 Cerebral cortex
Figure 3 Cerebral cortex
(Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3005/9074_B_7_5.jpg
Figure 4 Lung
Figure 4 Lung
(Alveolar cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Macrophages Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3005/9074_A_2_4.jpg
Figure 5 Colon
Figure 5 Colon
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3005/9074_A_7_3.jpg
Figure 6 Liver
Figure 6 Liver
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3005/9074_A_7_4.jpg
Figure 7 Kidney
Figure 7 Kidney
(Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3005/9074_A_7_5.jpg
Figure 8 Testis
Figure 8 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3005/9074_A_4_6.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (AN3-CA, HUVEC TERT2, NTERA-2, SK-MEL-30, TIME, U-266/84)
Figure 9 RNA cell line category: Cell line enhanced (AN3-CA, HUVEC TERT2, NTERA-2, SK-MEL-30, TIME, U-266/84)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000184588-PDE4B

Specifications

  • Immunogen
  • Full length human recombinant protein of human PDE4B produced in HEK293T cell
  • Host Species
  • Mouse
  • Type
  • Mouse IgG1
  • Specificity
  • PDE4B
  • Species Reactivity
  • Human, Mouse, Rat
  • Clone
  • 1D12
  • Applications
  • Western Blot, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-paraffin
  • Related Disease
  • Ocular Hypotension; Schizophrenia

Product Property

  • Purification
  • Immunogen affinity purified
  • Buffer
  • PBS, 1.0% BSA
  • Preservative
  • 50% Glycerol and 0.02% Sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • This antibody has been tested in Western blot (1/500-1/2000), Flow cytometry (1/100), Immunofluorescence (1/100), Immunocytochemistry (1/100), Immunohistochemistry and Immunohistochemistry-paraffin (1/150).

Target

  • Alternative Names
  • DPDE4; PDEIVB

Recommended Products

Isotype Control

Recommended Dilution Buffer

Secondary Antibody

Related Resources

  • Related Signaling Pathways

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "PDE4B"

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-2631 Hi-Affi™ Recombinant Rabbit Anti-PDE4B Monoclonal Antibody (DS2631AB) WB, FC IgG

Recombinant Antibody

CAT Product Name Application Type
VS1-YJ175 Rabbit Anti-PDE4B Antibody (VS-0722-YJ175) WB, IP Rabbit IgG

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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