Mouse Anti-PDE4B Recombinant Antibody (clone 1D12) (CAT#: VS-0224-XY99)
The product is a mouse monoclonal antibody (IgG1) which recognizes phosphodiesterase 4B of human, mouse and rat origin. It was purified by Immunogen affinity. It has been tested by Western blot, Flow cytometry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry and Immunohistochemistry-paraffin.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line AF22
(Immunofluorescent staining of human cell line AF22 shows localization to the Golgi apparatus.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3005/1786_F1_2_selected.jpg
(Immunofluorescent staining of human cell line AF22 shows localization to the Golgi apparatus.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3005/1786_F1_2_selected.jpg
Figure 2 IHC staining of human epididymis
(Immunohistochemical staining of human epididymis shows strong cytoplasmic positivity with granular pattern in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3005/ihc_selected.jpg
(Immunohistochemical staining of human epididymis shows strong cytoplasmic positivity with granular pattern in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3005/ihc_selected.jpg
Figure 3 Cerebral cortex
(Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3005/9074_B_7_5.jpg
(Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3005/9074_B_7_5.jpg
Figure 4 Lung
(Alveolar cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Macrophages Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3005/9074_A_2_4.jpg
(Alveolar cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Macrophages Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3005/9074_A_2_4.jpg
Figure 5 Colon
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3005/9074_A_7_3.jpg
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3005/9074_A_7_3.jpg
Figure 6 Liver
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3005/9074_A_7_4.jpg
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3005/9074_A_7_4.jpg
Figure 7 Kidney
(Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3005/9074_A_7_5.jpg
(Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3005/9074_A_7_5.jpg
Figure 8 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3005/9074_A_4_6.jpg
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3005/9074_A_4_6.jpg
Figure 9 RNA cell line category: Cell line enhanced (AN3-CA, HUVEC TERT2, NTERA-2, SK-MEL-30, TIME, U-266/84)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000184588-PDE4B
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000184588-PDE4B
Specifications
- Immunogen
- Full length human recombinant protein of human PDE4B produced in HEK293T cell
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- PDE4B
- Species Reactivity
- Human, Mouse, Rat
- Clone
- 1D12
- Applications
- Western Blot, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-paraffin
- Related Disease
- Ocular Hypotension; Schizophrenia
Product Property
- Purification
- Immunogen affinity purified
- Buffer
- PBS, 1.0% BSA
- Preservative
- 50% Glycerol and 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested in Western blot (1/500-1/2000), Flow cytometry (1/100), Immunofluorescence (1/100), Immunocytochemistry (1/100), Immunohistochemistry and Immunohistochemistry-paraffin (1/150).
Target
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Related Resources
cAMP Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2631 | Hi-Affi™ Recombinant Rabbit Anti-PDE4B Monoclonal Antibody (DS2631AB) | WB, FC | IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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