Mouse Anti-PDGFRB Recombinant Antibody (clone muM4TS.22) (CAT#: HPAB-S0107-YC)
Provided is an antibody muM4TS.22 that specific for the platelet-derived growth factor receptor β. muM4TS.22 does not bind to deletion proteins containing only the first through second domains of PDGF-R β, but does bind to protein D1-3 containing the third domain, showing that the binding epitope is in the third domain. muM4TS.22 effectively inhibited the stimulation of proliferation by PDGF BB, showing the ability of these antibodies to neutralize the biological activity of PDGF BB.
Specific Inquiry
Figure 1 M4TS.22 inhibited both PDGF BB binding to PDGFRb and PDGF BB-mediated proliferation. M4TS.11 and M4TS.22 were indistinguishable in their ability to inhibit PDGF BB-mediated proliferation.
A, effect of anti-PDGFRb antibodies on 125I-labeled PDGF BB binding to PDGFRb 1 cells. CHO C4 cells expressing the human PDGFRb were preincubated with 5 m g of the indicated antibody. Addition of 100 ng of unlabeled PDGF BB served to indicate the level of nonspecific 125I-labeled PDGF BB binding. B, effect of anti-PDGFRb antibodies on PDGF BB-mediated proliferation. CHO C4 cells were preincubated with various concentrations of M4TS.11 (●), M4TS.15 (▲), or M4TS.22 (■) for 2 h, after which PDGF BB was added.
Shulman, T., Sauer, F. G., Jackman, R. M., Chang, C. N., & Landolfi, N. F. (1997). An antibody reactive with domain 4 of the platelet-derived growth factor β receptor allows BB binding while inhibiting proliferation by impairing receptor dimerization. Journal of Biological Chemistry, 272(28), 17400-17404.
ELISA
Figure 2 Binding of the M4TS antibodies to PDGFRb domain deletion mutants. Reactivity of M4TS.11, M4TS.15, and M4TS.22 with various PDGFRb deletion mutants was determined by ELISA.
This analysis revealed that M4TS.22 was dependent on the presence of extracellular domain 3.
Shulman, T., Sauer, F. G., Jackman, R. M., Chang, C. N., & Landolfi, N. F. (1997). An antibody reactive with domain 4 of the platelet-derived growth factor β receptor allows BB binding while inhibiting proliferation by impairing receptor dimerization. Journal of Biological Chemistry, 272(28), 17400-17404.
Figure 3 Effect of anti-PDGFRb antibodies on PDGF BB-mediated proliferation of baboon and rabbit cells.
It demonstrates that preincubation with either M4TS.11 or M4TS.22 significantly decreased PDGF BB-mediated proliferation of the baboon SMC.
Shulman, T., Sauer, F. G., Jackman, R. M., Chang, C. N., & Landolfi, N. F. (1997). An antibody reactive with domain 4 of the platelet-derived growth factor β receptor allows BB binding while inhibiting proliferation by impairing receptor dimerization. Journal of Biological Chemistry, 272(28), 17400-17404.
Specifications
- Immunogen
- Soluble PDGF-R β
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human PDGFRB
- Species Reactivity
- Human
- Clone
- muM4TS.22
- Applications
- ELISA, Neut
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Platelet Derived Growth Factor Receptor Beta; Platelet-Derived Growth Factor Receptor, Beta Polypeptide; Beta-Type Platelet-Derived Growth Factor Receptor; Platelet-Derived Growth Factor Receptor 1; CD140 Antigen-Like Family Member B; PDGF-R-Beta; EC 2.7.10.1; PDGFR-Beta; PDGFR-1; PDGFR1; PDGFR; Platelet-Derived Growth Factor Receptor Beta
- Gene ID
- 5159
- UniProt ID
- P09619
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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| TAB-706CL | Anti-Human PDGFRB Antibody (IMC-2C5), Research Grade Biosimilar | Inhib, FuncS | Antibody |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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