Rabbit Anti-Phospho-RB1 (S780) Recombinant Antibody (clone 2E9) (CAT#: ZG-0544U)

Figure 1 Rabbit Anti-Phospho-RB1 (S780) Antibody (ZG-0544U) in IHC

IHC image of ZG-0544U diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Figure 2 Rabbit Anti-Phospho-RB1 (S780) Antibody (ZG-0544U) in IF

Immunofluorescence staining of K562 cells with ZG-0544U at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).

Figure 3 Rabbit Anti-Phospho-RB1 (S780) Antibody (ZG-0544U) in IP

Immunoprecipitating Phospho-RB1 in Hela whole cell lysate
Lane 1: Rabbit control IgG(1μg)instead of ZG-0544U in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: ZG-0544U(3μg)+ Hela whole cell lysate(1mg)
Lane 3: Hela whole cell lysate (20μg)