Which tau modifications does this antibody detect?

The PHF1 clone is attuned to phosphorylated tau, particularly at serine residues 396 and 404. This makes it instrumental in studying tauopathies, offering critical insights into tau's role in neurodegenerative conditions via precise detection in ELISA and WB assays.

Is the PHF1 clone suitable for immunohistochemistry?

Yes, the PHF1 clone is well-suited for IHC applications. It provides clarity in detecting phosphorylated tau arrangements in tissue samples, aiding scientists in deciphering neurodegenerative disease mechanisms through its strong affinity and precise binding capabilities.

Mouse Anti-phosphorylated Tau Recombinant Antibody (clone PHF1)

CAT#: VS-0623-WK88

The antibody clone PHF1 was produced by immunizing mouse with tau. It is specific for phosphorylated (p-)tau (serine 396 and 404). It can be used for detection of human tau in ELISA, WB, IHC and IF staining.

Published Data

IHC

Figure 1 Cell lysates and media transfected with iBs and scFvs were applied to paraffin-embedded sections of 10-month-old rTg4510 tau transgenic mice.

Cortical staining of neurofibrillary tangles was visualized with a biotinylated anti-c-myc secondary antibody. EGFP palys as a negative control.

ELISA

Figure 2 Cell lysates and media containing scFvs and iBs were subjected to direct ELISA.

Plates were coated with recombinant full-length tau, p-tau, or amyloid β as a control. Anti-c-myc HRP secondary antibody served as a detection.

FucnS

Figure 3 p-Tau-Specific iBs and scFvs Delay the Onset of Hindlimb Paralysis of Homozygous JNPL3 Mice.

JNPL3 mice for the survival cohort were intraspinally injected at P0 with pAAV2/8 (2 × 10e10 genomes) expressing PHF1, CP13, and Tau5 scFvs. Control mice were injected with rAAV-EGFP. All injected mice reached a terminal state (paralysis) within 12 months post-injection (n = 10-12). Median age of terminal state and relative increase of lifespan.

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Specifications

Immunogen
Tau

Host Species
Mouse

Type
Mouse IgG1

Specificity
Human tau

Species Reactivity
Human

Clone
PHF1

Applications
ELISA, WB, IHC, IF, FuncS

Conjugate
Unconjugated

Product Property

Clonality
Monoclonal

Purification
Affinity purified

Purity
>95% as determined by SDS-PAGE

Format
Liquid

Concentration
Please refer to the vial label for the specific concentration.

Buffer
PBS

Preservative
No Preservative

Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping
Ice packs

Applications

Application Notes
Immunohistochemical staining was done with cell lysates and media containing scFvs and iBs diluted 1/10 with PBS and applied to paraffin-fixed brain tissue slides from 10-month-old mice.

Target

Alternative Names
TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; tau-40; FTDP-17; PPP1R103; Tau-PHF6

Gene ID
4137

UniProt ID
P10636

Long Name
Microtubule associated protein tau

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Breast cancer biomarkers at key points during disease progression

Specific Detection

The phospho-tau antibody clone PHF1 excels in specificity for tau protein detection, particularly for its phosphorylated forms. We found it noteworthy for ELISA and western blots, enhancing the accuracy in studying neurodegenerative disease-related proteins.

Consistent Results

In our research, clone PHF1 has provided reproducible results across varying applications. Its ability to detect specific phosphorylation sites in tau proteins underscores its utility, particularly in understanding the intricacies of tau-oriented pathologies in our studies.

Q&As

Which tau modifications does this antibody detect?

A: The PHF1 clone is attuned to phosphorylated tau, particularly at serine residues 396 and 404. This makes it instrumental in studying tauopathies, offering critical insights into tau's role in neurodegenerative conditions via precise detection in ELISA and WB assays.
Is the PHF1 clone suitable for immunohistochemistry?

A: Yes, the PHF1 clone is well-suited for IHC applications. It provides clarity in detecting phosphorylated tau arrangements in tissue samples, aiding scientists in deciphering neurodegenerative disease mechanisms through its strong affinity and precise binding capabilities.

View the frequently asked questions answered by Creative Biolabs Support.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# VS-0623-WK88, RRID: AB_3714385)

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Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS-0623-WK88.pdf

MSDS

MSDS - VS-0623-WK88.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

Immunohistochemistry (IHC)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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