Mouse Anti-phosphorylated Tau Recombinant Antibody (clone PHF1) (CAT#: VS-0623-WK88)

Figure 1 Cell lysates and media transfected with iBs and scFvs were applied to paraffin-embedded sections of 10-month-old rTg4510 tau transgenic mice.

Cortical staining of neurofibrillary tangles was visualized with a biotinylated anti-c-myc secondary antibody. EGFP palys as a negative control.

Figure 2 Cell lysates and media containing scFvs and iBs were subjected to direct ELISA.

Plates were coated with recombinant full-length tau, p-tau, or amyloid β as a control. Anti-c-myc HRP secondary antibody served as a detection.

Figure 3 p-Tau-Specific iBs and scFvs Delay the Onset of Hindlimb Paralysis of Homozygous JNPL3 Mice.

JNPL3 mice for the survival cohort were intraspinally injected at P0 with pAAV2/8 (2 × 10e10 genomes) expressing PHF1, CP13, and Tau5 scFvs. Control mice were injected with rAAV-EGFP. All injected mice reached a terminal state (paralysis) within 12 months post-injection (n = 10-12). Median age of terminal state and relative increase of lifespan.