Mouse Anti-POU5F1 Recombinant Antibody (clone 41B1) (CAT#: VS11-QX120)
This product is a recombinant mouse antibody that recognizes POU5F1. This antibody can be used for the following applications: IHC, WB. OCT3/4 (also known as POU5F1) is a well-known marker for pluripotent stem cells, both physiologically and artificially induced. OCT3/4 is a transcription factor involved in self-renewal and pluripotency, and might counteract apoptosis in PGCs.
Specific Inquiry
Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line RT4
(Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/58267/1518_H6_1_selected.jpg
(Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/58267/1518_H6_1_selected.jpg
Figure 2 IHC staining of human hippocampus
(Immunohistochemical staining of human hippocampus shows strong nuclear positivity in glial cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25600/ihc_selected.jpg
(Immunohistochemical staining of human hippocampus shows strong nuclear positivity in glial cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25600/ihc_selected.jpg
Figure 3 Cerebral cortex
(Glial cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26380/57332_B_8_5.jpg
(Glial cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26380/57332_B_8_5.jpg
Figure 4 Colon
(Endothelial cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Nuclear
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26380/57332_A_7_3.jpg
(Endothelial cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Nuclear
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26380/57332_A_7_3.jpg
Figure 5 Liver
(Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26380/57332_A_7_4.jpg
(Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26380/57332_A_7_4.jpg
Figure 6 Pancreas
(Exocrine glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Pancreatic endocrine cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26380/57332_A_3_3.jpg
(Exocrine glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Pancreatic endocrine cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26380/57332_A_3_3.jpg
Figure 7 Kidney
(Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26380/57332_A_8_5.jpg
(Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26380/57332_A_8_5.jpg
Figure 8 Testis
(Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25600/54340_A_6_6.jpg
(Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25600/54340_A_6_6.jpg
Figure 9 Lymph node
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26380/57332_A_8_8.jpg
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26380/57332_A_8_8.jpg
Figure 10 RNA cell line category: Cell line enriched (SuSa)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000204531-POU5F1/subcellular
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000204531-POU5F1/subcellular
Specifications
- Immunogen
- Recombinant protein OCT3/4
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG2b, κ
- Specificity
- Human POU5F1
- Species Reactivity
- Human
- Clone
- 41B1
- Applications
- IHC, WB
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- 1 mg/mL (Please refer to the vial label for the specific concentration.)
- Buffer
- 0.2 μm filtered solution in PBS
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipped at 4°C.
Target
- Alternative Names
- OCT3; OCT4; OTF3; OTF4; OTF-3; Oct-3; Oct-4
- Gene ID
- 5460
- UniProt ID
- Q01860
- Long Name
- POU domain, class 5, transcription factor 1
- Sequence Similarities
- Belongs to the POU transcription factor family. Class-5 subfamily.
- Cellular Localization
- Nucleoplasm, Cytosol
- Function
- Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 or SOX15 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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| CAT | Product Name | Application | Type |
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| MOR-2797 | Hi-Affi™ Recombinant Rabbit Anti-POU5F1 Monoclonal Antibody (DS2797AB) | FC, IHC-P, IP, WB | IgG |
| MOR-0065-FY | Rabbit Anti-POU5F1 Recombinant Antibody (clone AFY0036) | FC, ICC, IHC-P, WB | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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