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    Mouse Anti-POU5F1 Recombinant Antibody (clone 41B1) (CAT#: VS11-QX120)

    This product is a recombinant mouse antibody that recognizes POU5F1. This antibody can be used for the following applications: IHC, WB. OCT3/4 (also known as POU5F1) is a well-known marker for pluripotent stem cells, both physiologically and artificially induced. OCT3/4 is a transcription factor involved in self-renewal and pluripotency, and might counteract apoptosis in PGCs.

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    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
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    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location and Protein Expression
    Figure 1 IF staining of human cell line RT4
    Figure 1 IF staining of human cell line RT4
    (Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/58267/1518_H6_1_selected.jpg
    Figure 2 IHC staining of human hippocampus
    Figure 2 IHC staining of human hippocampus
    (Immunohistochemical staining of human hippocampus shows strong nuclear positivity in glial cells.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/25600/ihc_selected.jpg
    Normal Tissue
    Figure 3 Cerebral cortex
    Figure 3 Cerebral cortex
    (Glial cells
    Staining: High
    Intensity: Strong
    Quantity: 75%-25%
    Location: Nuclear
    Neuronal cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear
    Neuropil
    Staining: Low
    Intensity: Weak
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/26380/57332_B_8_5.jpg
    Figure 4 Colon
    Figure 4 Colon
    (Endothelial cells
    Staining: Low
    Intensity: Weak
    Quantity:>75%
    Location: Nuclear
    Glandular cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear
    Peripheral nerve/ganglion
    Staining: Low
    Intensity: Weak
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/26380/57332_A_7_3.jpg
    Figure 5 Liver
    Figure 5 Liver
    (Cholangiocytes
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Nuclear
    Hepatocytes
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/26380/57332_A_7_4.jpg
    Figure 6 Pancreas
    Figure 6 Pancreas
    (Exocrine glandular cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear
    Pancreatic endocrine cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/26380/57332_A_3_3.jpg
    Figure 7 Kidney
    Figure 7 Kidney
    (Cells in glomeruli
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Nuclear
    Cells in tubules
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/26380/57332_A_8_5.jpg
    Figure 8 Testis
    Figure 8 Testis
    (Cells in seminiferous ducts
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Nuclear
    Leydig cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/25600/54340_A_6_6.jpg
    Figure 9 Lymph node
    Figure 9 Lymph node
    (Germinal center cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Nuclear
    Non-germinal center cells
    Staining: Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/26380/57332_A_8_8.jpg
    RNA Expression
    Figure 10 RNA cell line category: Cell line enriched (SuSa)
    Figure 10 RNA cell line category: Cell line enriched (SuSa)
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000204531-POU5F1/subcellular

    Specifications

    • Immunogen
    • Recombinant protein OCT3/4
    • Host Species
    • Mouse
    • Derivation
    • Mouse
    • Type
    • Mouse IgG2b, κ
    • Specificity
    • Human POU5F1
    • Species Reactivity
    • Human
    • Clone
    • 41B1
    • Applications
    • IHC, WB
    • Conjugate
    • Unconjugated

    Product Property

    • Clonality
    • Monoclonal
    • Purification
    • Protein A purified
    • Purity
    • >95% as determined by SDS-PAGE
    • Concentration
    • 1 mg/mL (Please refer to the vial label for the specific concentration.)
    • Buffer
    • 0.2 μm filtered solution in PBS
    • Storage
    • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
    • Shipping
    • Shipped at 4°C.

    Target

    • Alternative Names
    • OCT3; OCT4; OTF3; OTF4; OTF-3; Oct-3; Oct-4
    • Long Name
    • POU domain, class 5, transcription factor 1
    • Sequence Similarities
    • Belongs to the POU transcription factor family. Class-5 subfamily.
    • Cellular Localization
    • Nucleoplasm, Cytosol
    • Function
    • Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 or SOX15 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.

    Recommended Products

    Isotype Control

    Recommended Dilution Buffer

    Secondary Antibody

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

    See other products for "POU5F1"

    Select a product category from the dropdown menu below to view related products.
    Please select product type
    Rabbit Monoclonal Antibody Products IgG Antibody Products Cancer Stem Cell Biomarker Specific Antibody Products
    CAT Product Name Application Type
    MOR-2797 Hi-Affi™ Recombinant Rabbit Anti-POU5F1 Monoclonal Antibody (DS2797AB) FC, IHC-P, IP, WB IgG
    MOR-0065-FY Rabbit Anti-POU5F1 Recombinant Antibody (clone AFY0036) FC, ICC, IHC-P, WB Rabbit IgG

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