Rabbit Anti-PP2CA (phospho Y307) Recombinant Antibody (VS3-CJ1091) (CAT#: VS3-CJ1091)

• Immunogen
• Synthetic peptide conjugated to Keyhole Limpet Haemocyanin (KLH)

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human, Mouse, Rat phospho-PP2CA (Y307)

• Species Reactivity
• Human, Mouse, Rat

• Applications
• WB, ICC, IF, IHC, IP

• Conjugate
• Unconjugated

• Purification
• Protein A affinity purified

• Purity
• >95% as determined by SDS-PAGE

• Format
• Liquid

• Buffer
• 40% Glycerol, 1% BSA, TBS, pH7.4.

• Preservative
• 0.05% Sodium Azide

• Storage
• Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• This antibody has been tested for use in Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation.

• Alternative Names
• RP-C; PP2Ac; PP2CA; NEDLBA; PP2Calpha

• Gene ID
• 5515

• UniProt ID
• P67775

• Sequence Similarities
• Belongs to the PPP phosphatase family. PP-1 subfamily.

• Cellular Localization
• Centromere, Chromosome, Cytoplasm, Cytoskeleton, Nucleus

• Post Translation Modifications
• Reversibly methyl esterified on Leu-309 by leucine carboxyl methyltransferase 1 (LCMT1) and protein phosphatase methylesterase 1 (PPME1). Carboxyl methylation influences the affinity of the catalytic subunit for the different regulatory subunits, thereby modulating the PP2A holoenzyme's substrate specificity, enzyme activity and cellular localization.
  Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation.
  Polyubiquitinated, leading to its degradation by the proteasome.

• Protein Refseq
• NP_002706.1

• Function
• PP2A is the major phosphatase for microtubule-associated proteins (MAPs) (PubMed:22613722).
  PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase (PubMed:22613722).
  Cooperates with SGO2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity).
  Can dephosphorylate SV40 large T antigen and p53/TP53 (PubMed:17245430).
  Activates RAF1 by dephosphorylating it at 'Ser-259' (PubMed:10801873).
  Mediates dephosphorylation of WEE1, preventing its ubiquitin-mediated proteolysis, increasing WEE1 protein levels, and promoting the G2/M checkpoint (PubMed:33108758).
  Mediates dephosphorylation of MYC; promoting its ubiquitin-mediated proteolysis: interaction with AMBRA1 enhances interaction between PPP2CA and MYC (PubMed:25438055).
  Mediates dephosphorylation of FOXO3; promoting its stabilization: interaction with AMBRA1 enhances interaction between PPP2CA and FOXO3 (PubMed:30513302).

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Secondary Antibody

• 2ND-057CQ
• Donkey Anti-Rabbit IgG (H&L) Antibody

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody