Rabbit Anti-PRKAA1 (pS496) Recombinant Antibody (VS3-WK2266) (CAT#: VS3-WK2266)
This product is a rabbit recombinant antibody that is specific to phosphorylated PRKAA1. The target of this antibody is phospho-PRKAA1 (Ser496). It can be used in IP analysis at a dilution of 1/20, ICC analysis at a dilution of 1/50-1/200 and WB analysis at a dilution of 1/500-1/1000.
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We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35409/781_E12_2_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35409/781_E12_2_selected.jpg
Figure 2 IHC staining of human stomach
(Immunohistochemical staining of human stomach shows moderate cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64946/ihc_selected.jpg
(Immunohistochemical staining of human stomach shows moderate cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64946/ihc_selected.jpg
Figure 3 Colon
(Glandular cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64946/144077_A_7_3.jpg
(Glandular cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64946/144077_A_7_3.jpg
Figure 4 Liver
(Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64946/144077_A_7_4.jpg
(Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64946/144077_A_7_4.jpg
Figure 5 Gallbladder
(Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64946/144077_A_6_4.jpg
(Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64946/144077_A_6_4.jpg
Figure 6 Kidney
(Cells in tubules
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64946/144077_A_7_5.jpg
(Cells in tubules
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64946/144077_A_7_5.jpg
Figure 7 Testis
(Sertoli cells
Staining: Medium
Intensity: Moderate
Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64946/144077_A_6_6.jpg
(Sertoli cells
Staining: Medium
Intensity: Moderate
Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64946/144077_A_6_6.jpg
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000132356-PRKAA1/subcellular
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000132356-PRKAA1/subcellular
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Phospho-Ser496 of human PRKAA1
- Species Reactivity
- Human
- Applications
- WB, ICC, IP
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- IP: 1/20
WB: 1/500-1/1000
ICC: 1/50-1/200
Target
- Alternative Names
- PRKAA1; AMPK1; 5'-AMP-activated protein kinase catalytic subunit alpha-1; AMPK subunit alpha-1; Acetyl-CoA carboxylase kinase; ACACA kinase; Hydroxymethylglutaryl-CoA reductase kinase; HMGCR kinase; Tau-protein kinase PRKAA1
- Gene ID
- 5562
- UniProt ID
- Q13131
- Long Name
- Protein kinase AMP-activated catalytic subunit alpha 1
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2844 | Hi-Affi™ Recombinant Rabbit Anti-PRKAA1 Monoclonal Antibody (DS2844AB) | ELISA, IHC-P, WB | IgG |
| MOR-4672 | Hi-Affi™ Recombinant Rabbit Anti-PRKAA1 Monoclonal Antibody (TH186DS) | WB, IF, ICC | IgG |
| MOR-0064-FY | Rabbit Anti-PRKAA1 Recombinant Antibody (clone AFY0035) | ICC, IHC-P, WB | Rabbit IgG |
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