Human Anti-PRMT5 Recombinant Antibody (clone RAB-C136)
CAT#: VS11-QX124
This product is a recombinant human antibody that recognizes PRMT5. This antibody can be used for the following applications: ELISA, IF, IP-MS, ChIP.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5525/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human testis
Immunohistochemical staining of human testis shows moderate cytoplasmic and nuclear positivity in cells in seminiferous ducts and Leydig cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64708/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human duodenum
Immunohistochemical staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12459/27939_A_8_2_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5525/5_F2_1_blue_red_green.jpg
Subcellular Location and Protein Expression
Figure 5 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5525/4_F2_1_blue_red_green.jpg
Normal Tissue
Figure 6 Cerebral cortex
Glial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12459/27939_B_7_5.jpg
Normal Tissue
Figure 7 Colon
Glandular cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12459/27939_A_7_3.jpg
Normal Tissue
Figure 8 Kidney
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12459/27939_A_9_5.jpg
Normal Tissue
Figure 9 Testis
Cells in seminiferous ducts
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12459/27939_A_6_6.jpg
Normal Tissue
Figure 10 Lymph node
Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12459/27939_A_9_8.jpg
RNA Expression
Figure 11 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000100462-PRMT5
❮
❯
Specifications
- Immunogen
- PRMT5 protein
- Host Species
- Human
- Derivation
- Human
- Type
- Human IgG1, κ
- Specificity
- Human PRMT5
- Species Reactivity
- Human
- Clone
- RAB-C136
- Applications
- ELISA, IF, IP-MS, ChIP
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- 1 mg/mL (Please refer to the vial label for the specific concentration.)
- Buffer
- 0.2 μm filtered solution in PBS
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipped at 4°C.
Target
- Alternative Names
- Protein Arginine Methyltransferase 5; PRMT5; HSL7; JBP1; SKB1; IBP72; SKB1Hs; HRMT1L5
- Gene ID
- 10419
- UniProt ID
- O14744
- Long Name
- Protein arginine N-methyltransferase 5
- Sequence Similarities
- Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N-methyltransferase family.
- Cellular Localization
- Nucleoplasm, Cytosol
- Function
- Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "PRMT5"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-481 | Recombinant Human Anti-Human PRMT5 Antibody Fab Fragment, clone D245 | IF, ELISA, IP-MS, ChIP | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2869 | Hi-Affi™ Recombinant Rabbit Anti-PRMT5 Monoclonal Antibody (DS2869AB) | WB, IP, IHC-P, ICC, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1268-CN | Recombinant Mouse Anti-PRMT5 Monoclonal Antibody (7-C2) | WB, IHC | Mouse IgG2a |
| MRO-1269-CN | Recombinant Rabbit Anti-PRMT5 Monoclonal Antibody (CBACN-458) | WB, IF, IHC | Rabbit IgG |
| MRO-2138-CN | Rabbit Anti-PRMT5 Polyclonal Antibody (MRO-2138-CN) | WB, IF | Rabbit IgG |
| ZG-0525U | Rabbit Anti-PRMT5 Recombinant Antibody (clone 2H4) | ELISA, WB, IHC | Rabbit IgG |
| VS3-CJ126 | Rabbit Anti-PRMT5 Recombinant Antibody (VS3-CJ126) | WB, ICC, IF, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY409 | Mouse Anti-NHP PRMT5 Recombinant Antibody (clone PRMT5-21) | WB | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1762 | Anti-PRMT5 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5724 | Anti-Mouse PRMT5 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5723 | Anti-Human PRMT5 Immunohistochemistry Kit | IHC |
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