This product is a recombinant mouse antibody that can bind to human PSCA with high affinity.
Figure 1 Cell surface expression of PSCA detected by flow cytometry analysis using the anti-PSCA monoclonal antibody 8D11 (bold lines) or isotype control antibody (light solid lines).
Profiles are shown for the four transfected cell lines: PC3.gD.PSCA (A), MCF7.Her2.gD.PSCA (B), and HCT116.gD.PSCA clones 6 (C) and 8 (D).
Ross, S., Spencer, S. D., Holcomb, I., Tan, C., Hongo, J. A., & Devaux, B., et al. (2002). Prostate stem cell antigen as therapy target. Cancer Research, 62(9), 2546-2553.
Figure 2 In vitro cytotoxicity of the anti-PSCA immunoconjugate 8D11-DM1.
Shown is cell survival as a function of toxin concentration. Assays were performed in serum-containing (A–C) or serum-free (D) tissue culture medium. A, MCF7.Her2.gD.PSCA (•) and MCF7.Her2 (○); B, HCT116.gD.PSCA clone 6 (•), HCT116.gD.PSCA clone 8 (▴), and HCT116 (○); C and D, PC3.gD.PSCA (•) and PC3.neo (○). Data shown are the mean values of quadruplicate analyses.
Ross, S., Spencer, S. D., Holcomb, I., Tan, C., Hongo, J. A., & Devaux, B., et al. (2002). Prostate stem cell antigen as therapy target. Cancer Research, 62(9), 2546-2553.
Figure 3 A graph comparing the amount of Surface Versus internalized anti-PSCA monoclonal antibody for each of the antibodies as measured within 1 hour, by the intensity of the Cy3 fluorescence.
Figure 4 Shows the growth of PC-3.gDPSCA tumors in mice treated with 3 of the anti-PSCA antibodies (6F8, 8D11, and 5F2) as compared to the negative control, anti-ragweed antibody. The antibodies were administered by intraperito neal injection starting one day prior to inoculation with PSCA-transfected PC-3 cells, followed by biweekly injec tions following cell inoculation. “RX” under the arrows indicates the time point (day) of injection of the antibodies into the mice.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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CAT | Product Name | Application | Type |
---|---|---|---|
MOB-1981z | Mouse Anti-PSCA Recombinant Antibody (clone 24F5) | WB, FC, IF | Mouse IgG1, κ |
MOB-1641CT | Recombinant Mouse anti-Human PSCA Monoclonal antibody (EML781) | IHC-P, WB | |
HPAB-0074-CN | Mouse Anti-PSCA Recombinant Antibody (HPAB-0074-CN) | FC | Mouse IgG1, κ |
HPAB-0286CQ | Human Anti-PSCA Recombinant Antibody (clone mAb 5F2) | FC, IHC, FuncS | Human IgG1 |
HPAB-1309WJ | Mouse Anti-PSCA Recombinant Antibody (clone 6B8) | ELISA, WB | Mouse IgG |
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(Creative Biolabs Cat# HPAB-0287CQ, RRID: AB_3111382)
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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