Mouse Anti-PTEN Monoclonal Antibody (VS7-0425-WR708)
CAT#: VS7-0425-WR708
This product is a mouse IgG antibody that binds to human PTEN. The antibody can be used for WB, IF, ICC, FC, and ELISA assays. It cross reacts with mouse.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31335/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human testis
Immunohistochemical staining of human testis shows cytoplasmic positivity in cells in seminiferous ducts.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31335/134179_A_5_6_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Neuropil
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4076/12125_B_8_5.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Non-germinal center cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4076/12125_A_8_8.jpg
RNA Expression
Figure 5 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000171862-PTEN/subcellular
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Specifications
- Immunogen
- Recombinant human PTEN fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human PTEN
- Species Reactivity
- Human, Mouse
- Molecule Class
- Tumor Suppressor
- Applications
- WB, IF, ICC, FC, ELISA
- Conjugate
- Unconjugated
- Related Disease
- Cowden syndrome 1 (CWS1); Lhermitte-Duclos disease (LDD); Squamous cell carcinoma of the head and neck (HNSCC); Endometrial cancer (ENDMC); Glioma 2 (GLM2); Prostate cancer (PC); Macrocephaly/autism syndrome (MCEPHAS)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid
- Format
- Liquid
- Concentration
- 1 mg/ml
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunofluorescence: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- PTEN; TEP1; EC 3.1.3.16; EC 3.1.3.67; Mitochondrial PTENalpha; DEC; PTEN1; Phosphatase And Tensin-Like Protein; BZS; Phosphatidylinositol-3,4,5-Trisphosphate 3-Phosphatase And Dual-Specificity Protein Phosphatase PTEN; MHAM; Phosphatase And Tensin Homolog
- Gene ID
- 5728
- UniProt ID
- P60484
- Long Name
- Phosphatase and tensin homolog
- Cellular Localization
- Cell projection; Cytoplasm; Nucleus; Secreted; Synapse
- Post Translation Modifications
- Acetylation; Isopeptide bond; Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_000305.3
- Function
- Hydrolase; Protein phosphatase
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Related Diseases
Endometrial Cancer
Related Signaling Pathways
mTOR Signaling Pathway
PI3K-Akt Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "PTEN"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1640z | Mouse Anti-PTEN Recombinant Antibody (clone 27C11) | WB, ELISA, FC, ICC, IF | Mouse IgG1 |
| MOB-0338MZ | Recombinant Mouse Anti-Human PTEN Antibody (clone 7I3.2) | IHC | Mouse antibody |
| MOB-0231F | Mouse Anti-PTEN Recombinant Antibody (clone 2C10) | IHC-P, IF | Mouse IgG |
| MOB-0232F | Mouse Anti-PTEN Recombinant Antibody (clone 9E8) | IHC-P, IF | Mouse IgG |
| VS3-WK2147 | Rabbit Anti-PTEN (pS380) Recombinant Antibody (clone R08-7W6) | WB, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B021(A) | Recombinant Anti-mouse PTEN Intrabody [(D-Arg)9] | PCA, WB, FuncS | scFv-(D-Arg)9 |
| IAB-B021(G) | Recombinant Anti-mouse PTEN Intrabody [+36 GFP] | FACS, ELISA, IF, FuncS | scFv-(+36GFP) |
| IAB-B021(T) | Recombinant Anti-mouse PTEN Intrabody [Tat] | FACS, ICC, FuncS | scFv-Tat |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2915 | Hi-Affi™ Recombinant Rabbit Anti-PTEN Monoclonal Antibody (DS2915AB) | ICC, IHC-P, IP, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY183 | CytoStream™ Mouse Anti-PTEN Recombinant Antibody (VS-0225-XY183) | FC | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1816 | Anti-PTEN Immunohistochemistry Kit | IHC | |
| VS-0525-XY5823 | Anti-Mouse PTEN Immunohistochemistry Kit | IHC | |
| VS-0525-XY5822 | Anti-Human PTEN Immunohistochemistry Kit | IHC | |
| VS-0525-XY5824 | Anti-Rat PTEN Immunohistochemistry Kit | IHC |
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