Rabbit Anti-PTPN1 Recombinant Antibody (VS3-CJ733) (CAT#: VS3-CJ733)
This product is a rabbit antibody that recognizes human, and zebrafish PTPN1.
Specific Inquiry
Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12542/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12542/if_selected.jpg
Figure 2 IHC staining of human lymph node
(Immunohistochemical staining of human lymph node shows moderate cytoplasmic positivity in germinal center cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12542/30853_A_9_8_selected.jpg
(Immunohistochemical staining of human lymph node shows moderate cytoplasmic positivity in germinal center cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12542/30853_A_9_8_selected.jpg
Figure 3 Cerebral cortex
(Neuronal cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15217/36057_B_7_5.jpg
(Neuronal cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15217/36057_B_7_5.jpg
Figure 4 Kidney
(Cells in glomeruli
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15217/36057_A_7_5.jpg
(Cells in glomeruli
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15217/36057_A_7_5.jpg
Figure 5 Lymph node
(Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15217/36057_A_9_8.jpg
(Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15217/36057_A_9_8.jpg
Figure 6 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000196396-PTPN1/subcellular
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000196396-PTPN1/subcellular
Specifications
- Immunogen
- Recombinant protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Zebrafish PTPN1
- Species Reactivity
- Human, Zebrafish
- Applications
- WB, ICC, IF, IHC, FC
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 40% Glycerol, 1% BSA, TBS, pH7.4.
- Preservative
- 0.05% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry.
Target
- Alternative Names
- PTP1B
- Gene ID
- 5770
- UniProt ID
- P18031
- Sequence Similarities
- Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily.
- Cellular Localization
- Endoplasmic reticulum, Membrane
- Post Translation Modifications
- Oxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a sulfenamide cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction.
Ser-50 is the major site of phosphorylation as compared to Ser-242 and Ser-243. Activated by phosphorylation at Ser-50.
S-nitrosylation of Cys-215 inactivates the enzyme activity.
Sulfhydration at Cys-215 following endoplasmic reticulum stress inactivates the enzyme activity, promoting EIF2AK3/PERK activity.
- Protein Refseq
- NP_001265547.1; NP_002818.1
- Function
- Tyrosine-protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. Mediates dephosphorylation of EIF2AK3/PERK; inactivating the protein kinase activity of EIF2AK3/PERK. May play an important role in CKII- and p60c-src-induced signal transduction cascades. May regulate the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion. May also regulate the hepatocyte growth factor receptor signaling pathway through dephosphorylation of MET.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "PTPN1"
Select a product category from the dropdown menu below to view related products.
Please select product type
IgG Antibody Products
Chicken IgY Antibody Products
Rabbit Monoclonal Antibody Products
ScFv Antibody Products
Fab Antibody Products
Antibody Magnetic Beads
Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2288z | Mouse Anti-PTPN1 Recombinant Antibody (clone 24A4) | WB, FC, IF, IHC | Mouse IgG2a |
| VS3-XY1321 | Mouse Anti-PTPN1 Recombinant Antibody (clone 4F8F11) | IHC, FC | Mouse IgG1 |
| VS3-XY1323 | Mouse Anti-PTPN1 Recombinant Antibody (clone 2C11B12) | ELISA, WB, FC, IHC, ICC | Mouse IgG1 |
| VS3-XY1324 | Mouse Anti-PTPN1 Recombinant Antibody (clone 3G1A3) | ELISA, WB, FC | Mouse IgG1 |
| VS3-XY1325 | Mouse Anti-PTPN1 Recombinant Antibody (clone 5E9B2) | ELISA, WB | Mouse IgG2b |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for VS3-CJ733. Click the button above to contact us or submit your feedback about this product.
Popular products with customers
Application: WB, IF, IP, Neut, FuncS, ELISA, FC
Application: IF, IP, Neut, FuncS, ELISA, FC, WB
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: WB, FuncS, IF, Neut, ELISA, FC, IP
Application: WB, IP, IF, FuncS, FC, Neut, ELISA
Application: WB, ELISA, FC, IP, FuncS, IF, Neut
Application: SPR, Inhib, FuncS
Application: ELISA, IHC, FC, IP, IF, Inhib
Application: ELISA, Inhib, FuncS
Application: ELISA, WB, FC
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
