Rabbit Anti-PTPN1 Recombinant Antibody (VS3-CJ733)
CAT#: VS3-CJ733
This product is a rabbit antibody that recognizes human, and zebrafish PTPN1.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12542/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human lymph node
Immunohistochemical staining of human lymph node shows moderate cytoplasmic positivity in germinal center cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12542/30853_A_9_8_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15217/36057_B_7_5.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15217/36057_A_7_5.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15217/36057_A_9_8.jpg
RNA Expression
Figure 6 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000196396-PTPN1/subcellular
❮
❯
Specifications
- Immunogen
- Recombinant protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Zebrafish PTPN1
- Species Reactivity
- Human, Zebrafish
- Applications
- WB, ICC, IF, IHC, FC
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 40% Glycerol, 1% BSA, TBS, pH7.4.
- Preservative
- 0.05% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry.
Target
- Alternative Names
- PTP1B
- Gene ID
- 5770
- UniProt ID
- P18031
- Sequence Similarities
- Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily.
- Cellular Localization
- Endoplasmic reticulum, Membrane
- Post Translation Modifications
- Oxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a sulfenamide cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction.
Ser-50 is the major site of phosphorylation as compared to Ser-242 and Ser-243. Activated by phosphorylation at Ser-50.
S-nitrosylation of Cys-215 inactivates the enzyme activity.
Sulfhydration at Cys-215 following endoplasmic reticulum stress inactivates the enzyme activity, promoting EIF2AK3/PERK activity.
- Protein Refseq
- NP_001265547.1; NP_002818.1
- Function
- Tyrosine-protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. Mediates dephosphorylation of EIF2AK3/PERK; inactivating the protein kinase activity of EIF2AK3/PERK. May play an important role in CKII- and p60c-src-induced signal transduction cascades. May regulate the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion. May also regulate the hepatocyte growth factor receptor signaling pathway through dephosphorylation of MET.
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS3-CJ733. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "PTPN1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2288z | Mouse Anti-PTPN1 Recombinant Antibody (clone 24A4) | WB, FC, IF, IHC | Mouse IgG2a |
| MOB-0801CT | Recombinant Mouse anti-Human PTPN1 Monoclonal antibody (208BU700) | ELISA, WB | |
| HPAB-0374-CN | Chicken Anti-PTPN1 Recombinant Antibody (HPAB-0374-CN) | WB, ELISA, IP | Chicken IgY |
| HPAB-0375-CN | Chicken Anti-PTPN1 Recombinant Antibody (HPAB-0375-CN) | WB, ELISA, IP | Chicken IgY |
| ZG-0532U | Rabbit Anti-PTPN1 Recombinant Antibody (clone 1G3) | ELISA, WB, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0455MZ | Chicken Anti-PTPN1 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2928 | Hi-Affi™ Recombinant Rabbit Anti-PTPN1 Monoclonal Antibody (DS2928AB) | FC, ICC, IHC-P, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0374-CN-S(P) | Chicken Anti-PTPN1 Recombinant Antibody; scFv Fragment (HPAB-0374-CN-S(P)) | WB, ELISA, IP | Chicken scFv |
| HPAB-0375-CN-S(P) | Chicken Anti-PTPN1 Recombinant Antibody; scFv Fragment (HPAB-0375-CN-S(P)) | WB, ELISA, IP | Chicken scFv |
| PSBC-544 | Chicken Anti-PTPN1 Recombinant Antibody scFv Fragment (PSBC-544) | ELISA | Chicken scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0374-CN-F(E) | Chicken Anti-PTPN1 Recombinant Antibody; Fab Fragment (HPAB-0374-CN-F(E)) | WB, ELISA, IP | Chicken Fab |
| HPAB-0375-CN-F(E) | Chicken Anti-PTPN1 Recombinant Antibody; Fab Fragment (HPAB-0375-CN-F(E)) | WB, ELISA, IP | Chicken Fab |
| PFBC-544 | Chicken Anti-PTPN1 Recombinant Antibody Fab Fragment (PFBC-544) | ELISA | Chicken Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC842 | AbPlus™ Anti-PTPN1 Magnetic Beads (VS-0724-YC842) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC420 | Recombinant Anti-PTPN1 Vesicular Antibody, EV Displayed (VS-0425-YC420) | ELISA, FC, Inhib, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5858 | Anti-PTPN1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5859 | Anti-Rat PTPN1 Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: FuncS, Inhib, IP, ELISA
Application: WB, ELISA, FC, IHC, IP
-2-1.png)
Application: ELISA
Application: Neut, FC
Application: ELISA, WB, Neut, Funcs, IHC, Inhib
-2.png)
Application: ELISA, WB
Application: ELISA, FC, WB, Inhib, IHC
Application: Neut, FuncS, IHC, ELISA, FC, Inhib
Application: ELISA, WB
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
