Rabbit Anti-RELA (pS529) Recombinant Antibody (clone R03-6V6)
CAT#: VS3-WK2100
This product is a rabbit recombinant antibody that is specific to phosphorylated RELA. The target of this antibody is phospho-RELA (Ser529). The clone R03-6V6 can be used in WB analysis at a dilution of 1/500-1/1000, IP analysis at a dilution of 1/20, and FC analysis at a dilution of 1/50-1/100. The molecular weight of RELA (pSer529) observed in WB experiments using this antibody is 65 kDa.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/63461/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human fallopian tube
Immunohistochemical staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4264/18466_A_7_7_selected.jpg
Normal Tissue
Figure 3 Testis
Leydig cells Staining: Low Intensity: Moderate Quantity: <25% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: Medium Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4264/18466_A_5_6.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4264/18466_A_8_8.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4264/18809_B_8_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4264/18466_A_7_3.jpg
Normal Tissue
Figure 7 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4264/18466_A_8_5.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000173039-RELA
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Phospho-Ser529 of human RELA
- Species Reactivity
- Human
- Clone
- R03-6V6
- Applications
- WB, FC, IP
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% Glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IP: 1/20
FC: 1/50-1/100
Target
- Alternative Names
- RELA Proto-Oncogene, NF-KB Subunit; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 3; V-Rel Avian Reticuloendotheliosis Viral Oncogene Homolog A; Nuclear Factor NF-Kappa-B P65 Subunit; NFKB3; V-Rel Reticuloendotheliosis Viral Oncogene Homolog A
- Gene ID
- 5970
- UniProt ID
- Q04206
- Long Name
- RELA Proto-Oncogene, NF-KB Subunit
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
See other products for "RELA"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-4010z | Mouse Anti-RELA Recombinant Antibody (clone 11C6) | WB, FC, IHC | Mouse IgG1, κ |
| MOB-1712MZ | Recombinant Mouse Anti-Human RELA Antibody (clone 223B2032) | WB | Mouse antibody |
| MRO-2349-CN | Recombinant Rabbit Anti-RELA (phosphorylated Ser529) Monoclonal Antibody (SP07-00) | WB, IP, FC | Rabbit IgG |
| ZG-0313C | Mouse Anti-RELA Recombinant Antibody (ZG-0313C) | WB, ELISA | Mouse IgG |
| VS3-WK864 | Rabbit Anti-RELA Recombinant Antibody (VS3-WK864) | WB, IHC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B027(A) | Recombinant Anti-human RELA Intrabody [(D-Arg)9] | WB, CO-IP, FuncS | scFv-(D-Arg)9 |
| IAB-B027(G) | Recombinant Anti-human RELA Intrabody [+36 GFP] | IF, ELISA, FuncS | scFv-(+36GFP) |
| IAB-B027(T) | Recombinant Anti-human RELA Intrabody [Tat] | FC, IF, FuncS | scFv-Tat |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0722MZ | Chicken Anti-Nuclear factor NFκB, p65 subunit (RELA) Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3043 | Hi-Affi™ Recombinant Rabbit Anti-RELA Monoclonal Antibody (DS3043AB) | FC, IP, WB | IgG |
| MOR-4654 | Hi-Affi™ Recombinant Rabbit Anti-RELA Monoclonal Antibody (TH167DS) | WB, IHC-P, FC, ChIP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY223 | AbPlus™ Anti-RELA Magnetic Beads (CBACN-401) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC998 | CytoStream™ Rabbit Anti-RELA Recombinant Antibody (VS13-YC998) | WB, ICC, IF, IHC-P, FC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-FY6 | Rabbit Anti-RelA scFv-Fc Chimera (VS-0425-FY6) | WB, IHC-P, IF, IP | Rabbit IgG, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6072 | Anti-RELA Immunohistochemistry Kit | IHC | |
| VS-0525-XY6073 | Anti-Mouse RELA Immunohistochemistry Kit | IHC |
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