Mouse Anti-SDC1 Recombinant Antibody (clone 1A3A3)
CAT#: VS7-0425-WR833
This product is a mouse IgG1 antibody that binds to human SDC1. The antibody 1A3A3 recognizes specific amino acid domain of human SDC1 (AA: 28-171). It can be used for WB, ICC, and FC assays. It cross reacts with mouse.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to focal adhesion sites.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6185/if_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6185/40538_B_8_5.jpg
Normal Tissue
Figure 3 Colon
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6185/40538_A_7_3.jpg
Normal Tissue
Figure 4 Testis
Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6185/40538_A_4_6.jpg
Normal Tissue
Figure 5 Placenta
Syncytiotrophoblasts - cell body Staining: High Intensity: Strong Quantity:>75% Syncytiotrophoblasts - microvilli Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6185/40538_A_1_7.jpg
Normal Tissue
Figure 6 Lymph node
Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6185/40538_A_7_8.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (HBEC3-KT, hTCEpi, U-251 MG, U-266/70, U-266/84)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000115884-SDC1
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Specifications
- Immunogen
- Recombinant human SDC1 (AA: 28-171) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human SDC1
- Species Reactivity
- Human, Mouse
- Clone
- 1A3A3
- Molecule Class
- Transmembrane (Type I) Heparan Sulfate Proteoglycan
- Applications
- WB, ICC, FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunocytochemistry: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- SDC1; SDC; CD138; SYND1; Syndecan; CD138 Antigen; Syndecan Proteoglycan 1
- Gene ID
- 6382
- UniProt ID
- P18827
- Long Name
- Syndecan 1
- Cellular Localization
- Membrane; Secreted
- Post Translation Modifications
- Glycoprotein; Heparan sulfate; Phosphoprotein; Proteoglycan
- Protein Refseq
- NP_001006947.2
- Function
- Cell surface proteoglycan that contains both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix (By similarity).; Regulates exosome biogenesis in concert with SDCBP and PDCD6IP (PubMed:22660413).; Able to induce its own expression in dental mesenchymal cells and also in the neighboring dental epithelial cells via an MSX1-mediated pathway (By similarity).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
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