CytoStream™ Rabbit Anti-SDC1 Recombinant Antibody (VS13-YC1044)
CAT#: VS13-YC1044
The CytoStream™ Rabbit Anti-SDC1 Recombinant Antibody is specific for human, mouse and rat SDC1. SDC1 encodes a syndecan family proteoglycan mediating cellular interactions and implicated in tumor types via altered expression. The anti-SDC1 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/1000).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to focal adhesion sites.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6185/if_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6185/40538_B_8_5.jpg
Normal Tissue
Figure 3 Colon
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6185/40538_A_7_3.jpg
Normal Tissue
Figure 4 Testis
Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6185/40538_A_4_6.jpg
Normal Tissue
Figure 5 Placenta
Syncytiotrophoblasts - cell body Staining: High Intensity: Strong Quantity:>75% Syncytiotrophoblasts - microvilli Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6185/40538_A_1_7.jpg
Normal Tissue
Figure 6 Lymph node
Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6185/40538_A_7_8.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (HBEC3-KT, hTCEpi, U-251 MG, U-266/70, U-266/84)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000115884-SDC1
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Specifications
- Immunogen
- Recombinant human Syndecan 1, aa 13-274.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat SDC1
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, IHC-P, ICC, IF, FC
- Conjugate
- NONE
- Related Disease
- Monoclonal Gammopathy of Uncertain Significance and Endometritis
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity Chromatography purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/2000
Immunofluorescence (IF-cell): 1/100
Immunofluorescence (IF-tissue): 1/50
Immunohistochemistry-paraffin (IHC-P): 1/50-1/200
Flow cytometry (FC): 1/1000
Target
- Alternative Names
- Syndecan 1; Syndecan Proteoglycan 1; CD138 Antigen; SYND1; SDC; Heparan Sulfate Proteoglycan Fibroblast Growth Factor Receptor; Syndecan-1; Syndecan; CD138;
- Gene ID
- 6382
- UniProt ID
- P18827
- Long Name
- Syndecan 1
- Cellular Localization
- Membrane; Secreted
- Protein Refseq
- XP_005262677.1
- Function
- Cell surface proteoglycan that contains both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix (By similarity).
Regulates exosome biogenesis in concert with SDCBP and PDCD6IP (PubMed:22660413).
Able to induce its own expression in dental mesenchymal cells and also in the neighboring dental epithelial cells via an MSX1-mediated pathway.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "SDC1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2506z | Mouse Anti-SDC1 Recombinant Antibody (clone 22B3) | WB, ELISA, FC, ICC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-197 | Chimeric (Mouse/Human) Anti-SDC1 Recombinant Antibody (TAB-197) | Neut, ELISA, IF, IP, FuncS, FC, ICC | Chimeric (mouse/Human) IgG4, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-495CT | Mouse Anti-SDC1 Recombinant Antibody (TAB-495CT) | FC, WB | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-495CT-S(P) | Mouse Anti-SDC1 Recombinant Antibody; scFv Fragment (TAB-495CT-S(P)) | FC, WB | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-495CT-F(E) | Mouse Anti-SDC1 Recombinant Antibody; Fab Fragment (TAB-495CT-F(E)) | FC, WB | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0294MZ | Recombinant Mouse Anti-Human Syndecan 1 Antibody (clone NI16) | IHC | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3189 | Hi-Affi™ Recombinant Rabbit Anti-SDC1 Monoclonal Antibody (DS3189AB) | IHC-P | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0324-CN | Human Anti-SDC1 Recombinant Antibody (HPAB-0324-CN) | ELISA, FC | Humanized IgG3, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0326-CN | Human Anti-SDC1 Recombinant Antibody (HPAB-0326-CN) | ELISA, FC | Humanized IgG4, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0324-CN-S(P) | Human Anti-SDC1 Recombinant Antibody; scFv Fragment (HPAB-0324-CN-S(P)) | ELISA, FC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0326-CN-S(P) | Human Anti-SDC1 Recombinant Antibody; scFv Fragment (HPAB-0326-CN-S(P)) | ELISA, FC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0324-CN-F(E) | Human Anti-SDC1 Recombinant Antibody; Fab Fragment (HPAB-0324-CN-F(E)) | ELISA, FC | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0326-CN-F(E) | Human Anti-SDC1 Recombinant Antibody; Fab Fragment (HPAB-0326-CN-F(E)) | ELISA, FC | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-086CN | Human Anti-SDC1 Recombinant Antibody (NS-086CN) | ELISA | Humanized IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-087CN | Human Anti-SDC1 Recombinant Antibody (NS-087CN) | ELISA | Humanized IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-086CN-F(E) | Human Anti-SDC1 Recombinant Antibody; Fab Fragment (NS-086CN-F(E)) | ELISA | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-087CN-F(E) | Human Anti-SDC1 Recombinant Antibody; Fab Fragment (NS-087CN-F(E)) | ELISA | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-086CN-S(P) | Human Anti-SDC1 Recombinant Antibody; scFv Fragment (NS-086CN-S(P)) | ELISA | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-089CN | Mouse Anti-SDC1 Recombinant Antibody (NS-089CN) | ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-197 | Afuco™ Anti-SDC1 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-197) | Neut, ELISA, IF, IP, FuncS, FC | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-2546LY-F(E) | Human Anti-SDC1 Recombinant Antibody; Fab Fragment (HPAB-2546LY-F(E)) | ELISA | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-2546LY-S(P) | Human Anti-SDC1 Recombinant Antibody; scFv Fragment (HPAB-2546LY-S(P)) | ELISA | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-QX1001 | Mouse Anti-SDC1 Recombinant Antibody (clone 587CT7.3.6.5) | WB, ICC, FC | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY193 | CytoStream™ Mouse Anti-SDC1 Recombinant Antibody (VS-0225-XY193) | FC | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY16 | Human Anti-SDC1 scFv-Fc Chimera (VS-0325-FY16) | ELISA, FC, Cyt | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY153 | Human Anti-SDC1 scFv-Fc Chimera (VS-0325-FY153) | ADCC | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC134 | Recombinant Anti-SDC1 Vesicular Antibody, EV Displayed (VS-0425-YC134) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6401 | Anti-SDC1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6402 | Anti-Mouse SDC1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6404 | Anti-Rat SDC1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0825-YC300 | SmartAb™ Recombinant Anti-SDC1 pH-dependent Antibody (VS-0825-YC300) | ELISA, FC | Human IgG3 kappa |
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