What is the isotype of this chimeric antibody?

This Anti-SDC1 Recombinant Antibody (TAB-197) is a Chimeric (mouse/human) IgG4, kappa antibody. It combines mouse variable regions with human constant regions and is validated for a wide range of applications including IP, IF, FC, and FuncS.

Does this antibody recognize human SDC1?

Yes, this antibody specifically recognizes Human SDC1 (Syndecan-1). It was raised against the U266 cell line and has been extensively tested in ELISA, Neutralization, and other immunological methods.

Chimeric (Mouse/Human) Anti-SDC1 Recombinant Antibody (TAB-197)

CAT#: TAB-197

This product is a Chimeric (mouse/human) IgG4, κ antibody that can recognize human SDC1.

Tested Data Gene Expression

Figure 1 Anti-SDC1 Antibody (TAB-197) in WB

Western blot analysis of TAB-197 was performed with Syndecan-1/CD138 Protein, Human, Recombinant (His Tag).

TAB-197 incubation concentration: 2ng/μL.
The secondary antibody: HRP-Anti-Human IgG (H+L)
Lane 1: Reducing Antigen (0.1μg)
Lane 2: Reducing Antigen (0.3μg)
Lane 3: Reducing Antigen (0.6μg)

ELISA

Figure 2 Anti-SDC1 Antibody (TAB-197) in ELISA

ELISA analysis of TAB-197 was performed by coating with Syndecan-1/CD138 Protein, Human, Recombinant (His Tag).

The secondary antibody: HRP-Anti-Human IgG (H+L)

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Subcellular Location Figure 1 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to focal adhesion sites.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6185/if_selected.jpg

Normal Tissue Figure 2 Cerebral cortex

Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6185/40538_B_8_5.jpg

Normal Tissue Figure 3 Colon

Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6185/40538_A_7_3.jpg

Normal Tissue Figure 4 Testis

Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25%

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6185/40538_A_4_6.jpg

Normal Tissue Figure 5 Placenta

Syncytiotrophoblasts - cell body Staining: High Intensity: Strong Quantity:>75% Syncytiotrophoblasts - microvilli Staining: High Intensity: Strong

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6185/40538_A_1_7.jpg

Normal Tissue Figure 6 Lymph node

Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6185/40538_A_7_8.jpg

RNA Expression Figure 7 RNA cell line category: Cell line enhanced (HBEC3-KT, hTCEpi, U-251 MG, U-266/70, U-266/84)

Cell lines ordered by descending RNA expression order

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000115884-SDC1

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Specifications

Immunogen
U266 cell line

Host Species
Human

Derivation
Chimeric (mouse/Human)

Type
Chimeric (mouse/Human) IgG4, κ

Specificity
Human SDC1

Species Reactivity
Human

Applications
Neut, ELISA, IF, IP, FuncS, FC, ICC

Product Property

Purification
Protein A/G Purified

Purity
>95% as determined by SDS-PAGE and HPLC analysis

Concentration
Please refer to the vial label for the specific concentration

Buffer
PBS

Preservative
No preservatives

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
This antibody has been reported for use in Neutralization, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Immunoprecipitation, Functional Assay, Flow Cytometry, Immunocytochemistry.

Target

Alternative Names
SDC; CD138; SYND1; syndecan; syndecan-1; CD138 antigen; heparan sulfate proteoglycan fibroblast growth factor receptor; syndecan proteoglycan 1

Gene ID
6382

UniProt ID
P18827

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Breast cancer biomarkers at key points during disease progression

Excellent SDC1 Detection

This Chimeric Anti-SDC1 Recombinant Antibody is fantastic. We used it for Immunofluorescence and Flow Cytometry to detect Syndecan-1. The staining was bright and specific. As a chimeric IgG4, kappa antibody, it worked well with our secondary antibodies.

High Purity and Versatility

We validated this antibody for Immunoprecipitation and Functional Assays. It is highly pure (>95%) and preservative-free. It effectively pulled down SDC1 and worked well in neutralization studies. A very versatile reagent for researching cell adhesion.

Q&As

What is the isotype of this chimeric antibody?

A: This Anti-SDC1 Recombinant Antibody (TAB-197) is a Chimeric (mouse/human) IgG4, kappa antibody. It combines mouse variable regions with human constant regions and is validated for a wide range of applications including IP, IF, FC, and FuncS.
Does this antibody recognize human SDC1?

A: Yes, this antibody specifically recognizes Human SDC1 (Syndecan-1). It was raised against the U266 cell line and has been extensively tested in ELISA, Neutralization, and other immunological methods.

View the frequently asked questions answered by Creative Biolabs Support.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# TAB-197, RRID: AB_3111866)

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Biosimilar Overview

Please refer to Indatuximab Ravtansine Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Indatuximab Ravtansine.

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - TAB-197.pdf

MSDS

MSDS - TAB-197.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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