Mouse Anti-SEB Recombinant Antibody (clone pComb-49) (CAT#: VS-0323-CJ50)

• Immunogen
• Maltose-binding protein (MBP) fused with the N-terminal 15-mer peptide of SEB (N15-MBP protein)

• Host Species
• Mouse

• Type
• Mouse IgG

• Specificity
• Staphylococcal enterotoxin B (SEB)

• Species Reactivity
• S. aureus

• Clone
• pComb-49

• Applications
• Enzyme-linked Immunosorbent Assay, Western Blot, Surface Plasmon Resonance

• Conjugate
• Unconjugated

• Related Disease
• Food poisoning

• Purity
• >95% as determined by SDS-PAGE

• Buffer
• PBS

• Storage
• Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• ELISA: 0.5-1 μg/well
  Western Blot: 1-10 μg/well
  Surface Plasmon Resonance: 12 nM-30 nM

• Alternative Names
• Staphylococcal enterotoxin B

• UniProt ID
• P01552

• Sequence Similarities
• Belongs to the staphylococcal/streptococcal toxin family.

• Cellular Localization
• Secreted

• Protein Refseq
• WP_000278085.1

• Function
• Staphylococcal enterotoxin that activates the host immune system by binding as unprocessed molecules to major histocompatibility (MHC) complex class II and T-cell receptor (TCR) molecules. In turn, this ternary complex activates a large number of T-lymphocytes initiating a systemic release of pro-inflammatory cytokines (PubMed:2303780, PubMed:25015819).
  Causes also the intoxication staphylococcal food poisoning syndrome (By similarity).

Isotype Control

• MRO-A1591-YJ
• Mouse IgG Isotype control, Polyclonal

Secondary Antibody

• 2ND-040CQ
• Goat Anti-Mouse IgG (H&L) Antibody

• 2ND-042CQ
• Rabbit Anti-Mouse IgG (H&L) Antibody

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS

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