Mouse Anti-SHC1 Recombinant Antibody (clone 2F7C7) (CAT#: VS7-0425-WR852)
This product is a mouse IgG1 antibody that binds to human SHC1. The antibody 2F7C7 recognizes specific amino acid domain of human SHC1 (AA: 385-495). It can be used for WB, ICC, and FC assays. It cross reacts with mouse.
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

(Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5374/if_selected.jpg

(Immunofluorescent staining of human cell line A-431 shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/16305/if_selected.jpg

(Immunohistochemical staining of human placenta shows cytoplasmic positivity in trophoblastic cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1844/ihc_selected.jpg

(Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5374/ihc_selected.jpg

(Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/16305/ihc_selected.jpg

(Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Round or early spermatids Staining: Medium Intensity: Moderate Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/16305/36379_A_6_6.jpg

(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/16305/36379_A_7_8.jpg

(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/16305/36379_A_7_3.jpg

(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/16305/36379_A_7_5.jpg

(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000160691-SHC1
Specifications
- Immunogen
- Recombinant human SHC1 (AA: 385-495) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human SHC1
- Species Reactivity
- Human, Mouse
- Clone
- 2F7C7
- Molecule Class
- Adapter Protein
- Applications
- WB, ICC, FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunocytochemistry: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- SHC1; SHCA; Src Homology 2 Domain-Containing-Transforming Protein C1; SHC-Transforming Protein A; SHC; SH2 Domain Protein C1; SHC-Transforming Protein 1
- Gene ID
- 6464
- UniProt ID
- P29353
- Long Name
- SHC adaptor protein 1
- Cellular Localization
- Cell junction; Cytoplasm; Mitochondrion
- Post Translation Modifications
- Acetylation; Phosphoprotein
- Protein Refseq
- NP_001123512.1
- Function
- Signaling adapter that couples activated growth factor receptors to signaling pathways. Participates in a signaling cascade initiated by activated KIT and KITLG/SCF. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p46Shc and isoform p52Shc may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shc does not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular response to oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and is indispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis. The expression of isoform p66Shc has been correlated with life span (By similarity).; Participates in signaling downstream of the angiopoietin receptor TEK/TIE2, and plays a role in the regulation of endothelial cell migration and sprouting angiogenesis
Recommended Products
Isotype Control
Secondary Antibody
Reconstitution Buffer
Recommended Dilution Buffer
Related Resources
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "SHC1"
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CAT | Product Name | Application | Type |
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MOB-1856MZ | Recombinant Mouse Anti-Human SHC1 Antibody (clone 3F4) | IHC-P, WB | Mouse antibody |
VS3-QX1008 | Mouse Anti-SHC1 Recombinant Antibody (clone 1C4-F0-E4) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
VS3-QX1009 | Mouse Anti-SHC1 Recombinant Antibody (clone 7C-7F) | WB, ICC, FC | Mouse IgG1 |
VS3-XY1419 | Mouse Anti-SHC1 Recombinant Antibody (clone 2C1) | ELISA, WB, FC, ICC | Mouse IgG1 |
VS3-XY3505 | Rabbit Anti-SHC1 Recombinant Antibody (clone R02-1J3) | WB, IHC-P, ICC, IF, FC | Rabbit IgG |
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