Anti-SIRT1 Immunohistochemistry Kit
CAT#: VS-0325-XY2070
The SIRT1 IHC Kit is a great option for immunohistochemical studies. It includes all necessary reagents, making it easy to start experiments. It is applicable to both paraffin - and frozen - section - based immunohistochemistry. The kit's antibodies are optimized to specifically recognize SIRT1, enabling accurate determination of SIRT1 expression levels, which is important for understanding its role in cellular metabolism, aging, and stress resistance.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6295/43_F3_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/52351/826_A6_1_selected.jpg
Normal Tissue
Figure 3 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6295/18379_A_4_6.jpg
Normal Tissue
Figure 4 Adrenal gland
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6295/18379_B_6_5.jpg
RNA Expression
Figure 5 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000096717-SIRT1
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Specifications
- Application
- IHC
- Size
- 50 Tests
- Species Reactivity
- Human, Mouse, Rat
- Target
- SIRT1
- Primary Antibody
- Rabbit Anti-SIRT1 Antibody
- Secondary Antibody
- Goat anti-Rabbit Antibody, HRP
- Sample Type
- FFPE tissue; Frozen section tissue
- Kit Storage
- All reagents should be kept at 2-8°C. The kit remains stable for up to 6 months after arrival.
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Datasheet
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Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "SIRT1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3980z | Mouse Anti-SIRT1 Recombinant Antibody (clone 4G3) | WB, ELISA, FC, ICC, IF, IHC | Mouse IgG1 |
| ZG-0071U | Rabbit Anti-SIRT1 Recombinant Antibody (clone 9E9) | ELISA, IHC, IF | Rabbit IgG |
| VS3-WK1101 | Rabbit Anti-SIRT1 Recombinant Antibody (VS3-WK1101) | WB, ICC, IF, IHC, IP | Rabbit IgG |
| VS3-FY1338 | Recombinant Rabbit Anti-Sirt1 Antibody (clone R06-4K9) | WB | Rabbit IgG |
| VS7-0425-WR854 | Mouse Anti-SIRT1 Monoclonal Antibody (VS7-0425-WR854) | WB, IHC-P, IF, ICC, FC, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0777MZ | Chicken Anti-SIRT1 Polyclonal IgY | IHC, IF, WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3267 | Hi-Affi™ Recombinant Rabbit Anti-SIRT1 Monoclonal Antibody (DS3267AB) | IP, WB | IgG |
| MOR-0043-FY | Rabbit Anti-SIRT1 Recombinant Antibody (clone AFY0014) | ICC, IHC, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY235 | AbPlus™ Anti-SIRT1 Magnetic Beads (CBACN-504) | IP, Protein Purification | |
| VS-0724-YC526 | AbPlus™ Anti-SIRT1 Magnetic Beads (VS-0724-YC526) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY480 | Mouse Anti-NHP SIRT1 Recombinant Antibody (clone 1F3) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6601 | Anti-Human SIRT1 Immunohistochemistry Kit | IHC |
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