Mouse Anti-NHP SIRT1 Recombinant Antibody (clone 1F3) (CAT#: VS-1024-XY480)
Engineered to recognize SIRT1, this antibody demonstrates outstanding reactivity with both human and non-human primate specimens. Utilizing a purified recombinant fragment of human SIRT1 as the immunogen, it delivers high specificity and reliability across various applications, including Western blotting, immunohistochemistry, immunocytochemistry, flow cytometry, and ELISA. Its robust performance makes it an essential tool for researchers exploring the regulatory roles of SIRT1 in cellular metabolism and longevity, facilitating advancements in the understanding of epigenetic mechanisms.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6295/43_F3_2_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6295/43_F3_2_selected.jpg
Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/52351/826_A6_1_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/52351/826_A6_1_selected.jpg
Figure 3 Testis
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6295/18379_A_4_6.jpg
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6295/18379_A_4_6.jpg
Figure 4 Adrenal gland
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6295/18379_B_6_5.jpg
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6295/18379_B_6_5.jpg
Figure 5 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000096717-SIRT1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000096717-SIRT1
Specifications
- Immunogen
- Purified recombinant fragment of human SIRT1
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- SIRT1
- Species Reactivity
- Human, Non-human primate
- Clone
- 1F3
- Applications
- WB, IHC, ICC, FC, ELISA
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:500-1:1000, IHC: 1:200-1:800, ICC: 1:200-1:800, FC: 1:200-1:400, ELISA: 1:8000
Target
Recommended Products
Recommended Dilution Buffer
Isotype Control
Secondary Antibody
Related Resources
Amphetamine Addiction
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1383CT | Recombinant Mouse anti-Human SIRT1 Monoclonal antibody (2G4) | ELISA, FC, ICC/IF, IHC-P, WB | |
| VS3-QX1010 | Mouse Anti-SIRT1 Recombinant Antibody (clone 2G10-F4-D9) | WB, IP | Mouse IgG2b |
| VS3-QX1011 | Mouse Anti-SIRT1 Recombinant Antibody (clone 1089CT5.3.1) | WB | Mouse IgG3, κ |
| VS3-WK1101 | Rabbit Anti-SIRT1 Recombinant Antibody (VS3-WK1101) | WB, ICC, IF, IHC, IP | Rabbit IgG |
| VS7-0425-WR854 | Mouse Anti-SIRT1 Monoclonal Antibody (VS7-0425-WR854) | WB, IHC-P, IF, ICC, FC, ELISA | Mouse IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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