Human Anti-SLC30A10 Recombinant Antibody (clone C22) (CAT#: HPAB-M0205-YC)
The type-1 diabetes mellitus resistant ZnT8 specific antibody C22 is screened and authenticated and can be used for diagnosing and treating type-1 diabetes mellitus and/or preparing prognosis reagents.
Specific Inquiry
Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
ELISA
Figure 1 Binding ability of scFv antibodies to ZnT8 N/C fusion protein by phage-ELISA.
The affinity of scFv C27 and C22 were the highest among these positive clones.
Wu, Q., Wang, X., Gu, Y., Zhang, X., Qin, Y., Chen, H., ... & Zhang, M. (2016). Screening and identification of human ZnT8-specific single-chain variable fragment (scFv) from type 1 diabetes phage display library. Science China Life Sciences, 59, 686-693.
WB
Figure 2 Binding ability of scFv to ZnT8 N/C fusion protein by western blotting analyze.
Lane 1, N/C fusion protein detected with scFv-C22; lane 2, negative control. ZnT8 N/C fusion protein showed a band ~20 kD with C22 scFv, which is the predicted molecular weight.
Wu, Q., Wang, X., Gu, Y., Zhang, X., Qin, Y., Chen, H., ... & Zhang, M. (2016). Screening and identification of human ZnT8-specific single-chain variable fragment (scFv) from type 1 diabetes phage display library. Science China Life Sciences, 59, 686-693.
Figure 3 Affinity test of C22-scFv to ZnT8 N/C fusion protein.
The affinity assay showed that C22 scFv bound to N/C fusion protein with high affinity.
Wu, Q., Wang, X., Gu, Y., Zhang, X., Qin, Y., Chen, H., ... & Zhang, M. (2016). Screening and identification of human ZnT8-specific single-chain variable fragment (scFv) from type 1 diabetes phage display library. Science China Life Sciences, 59, 686-693.
ELISA
Figure 4 Binding capacity of scFv to C terminal dimer protein measured by ELISA.
The binding capacity of serial diluted scFv to the C terminal dimer decreased with the dilution ratio increased, which indicated the renatured scFvs could bind to ZnT8 in a concentration dependent manner.
Wu, Q., Wang, X., Gu, Y., Zhang, X., Qin, Y., Chen, H., ... & Zhang, M. (2016). Screening and identification of human ZnT8-specific single-chain variable fragment (scFv) from type 1 diabetes phage display library. Science China Life Sciences, 59, 686-693.
IHC
Figure 5 Immunohistochemical reaction to ZnT8 in the human pancreas.
The C22-scFv were specific to human islet cells.
Wu, Q., Wang, X., Gu, Y., Zhang, X., Qin, Y., Chen, H., ... & Zhang, M. (2016). Screening and identification of human ZnT8-specific single-chain variable fragment (scFv) from type 1 diabetes phage display library. Science China Life Sciences, 59, 686-693.
Specifications
- Host Species
- Human
- Derivation
- Humanized
- Type
- Humanized IgG
- Specificity
- Human SLC30A10
- Species Reactivity
- Human
- Clone
- C22
- Applications
- ELISA, WB, IHC
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Solute Carrier Family 30 Member 10; Manganese Transporter SLC30A10; Zinc Transporter 8; ZnT-10; ZNT10; ZNT8;
- Gene ID
- 55532
- UniProt ID
- Q6XR72
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "Clone C22"
See other products for "SLC30A10"
Select a product category from the dropdown menu below to view related products.
Please select product type
IgG Antibody Products
Fab Antibody Products
ScFv Antibody Products
Mouse Antibody Products
Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1754z | Recombinant Anti-Human SLC30A10 Antibody | WB, IF, FuncS | IgG |
| HPAB-M0206-YC | Human Anti-SLC30A10 Recombinant Antibody (clone C27) | ELISA, WB, IHC | Human IgG |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for HPAB-M0205-YC. Click the button above to contact us or submit your feedback about this product.
Popular products with customers
Application: WB, ELISA, IP, FC, FuncS, Neut, IF
Application: Neut, ELISA, IF, IP, FuncS, FC, IHC
Application: WB, ELISA, FC, IP, FuncS, IF, Neut
Application: WB, FC, IP, ELISA, Neut, FuncS, IF
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
Application: Neut, Inhib, FC, ELISA
Application: FC, FRET, Internalization
Application: Neut, ELISA, FuncS
Application: ELISA, FuncS
Application: ELISA, FC, IF, IHC, WB
Application: ELISA, Inhib, FuncS
Application: ELISA, FC, Neut, Inhib
Application: IF, IP, Neut, FuncS, ELISA, FC
Application: ELISA, FC
For Research Use Only. Not For Clinical Use.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
