Human Anti-SLC30A10 Recombinant Antibody (clone C22) (CAT#: HPAB-M0205-YC)
The type-1 diabetes mellitus resistant ZnT8 specific antibody C22 is screened and authenticated and can be used for diagnosing and treating type-1 diabetes mellitus and/or preparing prognosis reagents.
Specific Inquiry
ELISA
Figure 1 Binding ability of scFv antibodies to ZnT8 N/C fusion protein by phage-ELISA.
The affinity of scFv C27 and C22 were the highest among these positive clones.
Wu, Q., Wang, X., Gu, Y., Zhang, X., Qin, Y., Chen, H., ... & Zhang, M. (2016). Screening and identification of human ZnT8-specific single-chain variable fragment (scFv) from type 1 diabetes phage display library. Science China Life Sciences, 59, 686-693.
WB
Figure 2 Binding ability of scFv to ZnT8 N/C fusion protein by western blotting analyze.
Lane 1, N/C fusion protein detected with scFv-C22; lane 2, negative control. ZnT8 N/C fusion protein showed a band ~20 kD with C22 scFv, which is the predicted molecular weight.
Wu, Q., Wang, X., Gu, Y., Zhang, X., Qin, Y., Chen, H., ... & Zhang, M. (2016). Screening and identification of human ZnT8-specific single-chain variable fragment (scFv) from type 1 diabetes phage display library. Science China Life Sciences, 59, 686-693.
Figure 3 Affinity test of C22-scFv to ZnT8 N/C fusion protein.
The affinity assay showed that C22 scFv bound to N/C fusion protein with high affinity.
Wu, Q., Wang, X., Gu, Y., Zhang, X., Qin, Y., Chen, H., ... & Zhang, M. (2016). Screening and identification of human ZnT8-specific single-chain variable fragment (scFv) from type 1 diabetes phage display library. Science China Life Sciences, 59, 686-693.
ELISA
Figure 4 Binding capacity of scFv to C terminal dimer protein measured by ELISA.
The binding capacity of serial diluted scFv to the C terminal dimer decreased with the dilution ratio increased, which indicated the renatured scFvs could bind to ZnT8 in a concentration dependent manner.
Wu, Q., Wang, X., Gu, Y., Zhang, X., Qin, Y., Chen, H., ... & Zhang, M. (2016). Screening and identification of human ZnT8-specific single-chain variable fragment (scFv) from type 1 diabetes phage display library. Science China Life Sciences, 59, 686-693.
IHC
Figure 5 Immunohistochemical reaction to ZnT8 in the human pancreas.
The C22-scFv were specific to human islet cells.
Wu, Q., Wang, X., Gu, Y., Zhang, X., Qin, Y., Chen, H., ... & Zhang, M. (2016). Screening and identification of human ZnT8-specific single-chain variable fragment (scFv) from type 1 diabetes phage display library. Science China Life Sciences, 59, 686-693.
Specifications
- Host Species
- Human
- Derivation
- Humanized
- Type
- Humanized IgG
- Specificity
- Human SLC30A10
- Species Reactivity
- Human
- Clone
- C22
- Applications
- ELISA, WB, IHC
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Solute Carrier Family 30 Member 10; Manganese Transporter SLC30A10; Zinc Transporter 8; ZnT-10; ZNT10; ZNT8;
- Gene ID
- 55532
- UniProt ID
- Q6XR72
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Mouse Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1754z | Recombinant Anti-Human SLC30A10 Antibody | WB, IF, FuncS | IgG |
| HPAB-M0206-YC | Human Anti-SLC30A10 Recombinant Antibody (clone C27) | ELISA, WB, IHC | Human IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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