AibGenesis™ Rabbit Anti-SPI1 Recombinant Antibody (AibG-Rb-SP762)
CAT#: VS-0426-FY762
Created using an AI-powered antibody discovery platform, this SPI1 antibody supports AML research, providing broad cross-species reactivity for WB and IHC.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line REH
Immunofluorescent staining of human cell line REH shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44653/1676_D3_2_selected.jpg
Normal Tissue
Figure 2 Bone marrow
High expression
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44653/125448_B_6_4_val_selected.jpg
Normal Tissue
Figure 3 Colon
Enterocytes - Mucosal lymphoid cells Staining: Medium Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4079/11463_A_9_3.jpg
Normal Tissue
Figure 4 Skin
Fibrohistiocytic cells Staining: High Intensity: Strong Langerhans cells Staining: Medium Intensity: Strong Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4079/11463_B_9_1.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4079/11463_A_9_8.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (HEL, HL-60, HMC-1, NB-4, THP-1, U-698, U-937)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000066336-SPI1
❮
❯
Specifications
- Immunogen
- Human
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human SPI1
- Species Reactivity
- Human, Mouse, Hamster, Rat
- Clone
- AibG-Rb-SP762
- Applications
- Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
- Conjugate
- Unconjugated, Biotin and FITC are available.
- Related Disease
- Acute Myeloid Leukemia
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity purified
- Format
- Liquid
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% Protective protein
- Preservative
- 0.01% Sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- Western Blot (1:500-1:1000), Immunohistochemistry (1:50-1:100), Immunocytochemistry/Immunofluorescence (1:50-1:200), Immunoprecipitation (1:20-1:50)
Target
- Alternative Names
- Dis1; PU.1; Dis-1; Sfpi1; Spi-1; Sfpi-1; Tcfpu1; Tfpu.1
- Gene ID
- 20375
- UniProt ID
- P17433
- Long Name
- Spi-1 Proto-Oncogene
- Sequence Similarities
- Belongs to the ETS family.
- Cellular Localization
- Nucleus
- Protein Refseq
- NP_001074016.1; NP_003111.2
- Function
- Pioneer transcription factor, which controls hematopoietic cell fate by decompacting stem cell heterochromatin and allowing other transcription factors to enter otherwise inaccessible genomic sites. Once in open chromatin, can directly control gene expression by binding genetic regulatory elements and can also more broadly influence transcription by recruiting transcription factors, such as interferon regulatory factors (IRFs), to otherwise inaccessible genomic regions (PubMed:23658224, PubMed:33951726).
Transcriptionally activates genes important for myeloid and lymphoid lineages, such as CSF1R (By similarity).
Transcriptional activation from certain promoters, possibly containing low affinity binding sites, is achieved cooperatively with other transcription factors. FCER1A transactivation is achieved in cooperation with GATA1 (By similarity).
May be particularly important for the pro- to pre-B cell transition (PubMed:33951726).
Binds (via the ETS domain) onto the purine-rich DNA core sequence 5'-GAGGAA-3', also known as the PU-box (PubMed:33951726).
In vitro can bind RNA and interfere with pre-mRNA splicing (By similarity).
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS-0426-FY762. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "SPI1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-4037z | Mouse Anti-SPI1 Recombinant Antibody (clone 24D1) | WB, IP, IF, IHC, ELISA | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1727CT | Recombinant Mouse anti-Human SPI1 Monoclonal antibody (2G1) | ELISA, WB |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3386 | Hi-Affi™ Recombinant Rabbit Anti-SPI1 Monoclonal Antibody (DS3386AB) | FC, IHC-P, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1297-CN | Recombinant Rabbit Anti-SPI1 Monoclonal Antibody (JB72-35) | WB, IHC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-QX1049 | Mouse Anti-SPI1 Recombinant Antibody (clone 5C7) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-WK326 | Rabbit Anti-SPI1 Recombinant Antibody (clone JB72-35) | WB, ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC1105 | CytoStream™ Rabbit Anti-SPI1 Recombinant Antibody (VS13-YC1105) | WB, IHC-P, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2162 | Anti-SPI1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6860 | Anti-Human SPI1 Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: WB, FuncS, IF, Neut, ELISA, FC, IP
Application: WB, ELISA, IP, FC, FuncS, Neut, IF
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
Application: WB, ELISA, FC, IP, FuncS, IF, Neut
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
Application: IF, IP, Neut, FuncS, ELISA, FC, WB
Application: WB, FC, IP, ELISA, Neut, FuncS, IF
Application: ELISA, IP, FC, FuncS, Neut, IF, ICC
Application: WB, FC, IP, ELISA, Neut, FuncS, IF
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: Neut, ELISA, IF, IP, FuncS, FC, WB
Application: Neut, ELISA, FuncS
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
