Mouse Anti-STAT5A Recombinant Antibody (clone 7F7A4) (CAT#: VS7-0425-WR928)
This product is a mouse IgG1 antibody that binds to human STAT5A. The antibody 7F7A4 recognizes specific amino acid domain of human Stat5 (AA: KAVDG[pTyr]VKPQIK). It can be used for FC assay.
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IHC staining of human lymph node
(Immunohistochemical staining of human lymph node shows strong nuclear positivity in non-germinal center cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/42128/ihc_selected.jpg
(Immunohistochemical staining of human lymph node shows strong nuclear positivity in non-germinal center cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/42128/ihc_selected.jpg
Figure 2 Soft tissue
(Peripheral nerve
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3860/11333_B_2_8.jpg
(Peripheral nerve
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3860/11333_B_2_8.jpg
Figure 3 Lymph node
(Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3860/11333_A_8_8.jpg
(Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3860/11333_A_8_8.jpg
Figure 4 RNA cell line category: Cell line enhanced (HDLM-2, HEL, HMC-1, K-562, MOLT-4)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000126561-STAT5A/subcellular
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000126561-STAT5A/subcellular
Specifications
- Immunogen
- Recombinant human Stat5 (AA: KAVDG[pTyr]VKPQIK) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human STAT5A
- Species Reactivity
- Human
- Clone
- 7F7A4
- Molecule Class
- STAT (Signal Transducers And Activators Of Transcription) Protein
- Applications
- FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- MGF; STAT5
- Gene ID
- 6776
- UniProt ID
- P42229
- Long Name
- Signal transducer and activator of transcription 5A
- Cellular Localization
- Cytoplasm; Nucleus
- Post Translation Modifications
- Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_001275647.1
- Function
- Activator; DNA-binding
Recommended Products
Isotype Control
Secondary Antibody
Reconstitution Buffer
Recommended Dilution Buffer
Related Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2044z | Mouse Anti-STAT5A Recombinant Antibody (clone 30F6) | WB, FC, ICC, IF, IHC | Mouse IgG1 |
| MRO-1441-CN | Recombinant Rabbit Anti-STAT5A Monoclonal Antibody (CBACN-532) | WB, IF, IHC, IP, FC | Rabbit IgG |
| ZG-0585U | Rabbit Anti-Phospho-STAT5A (Y694) Recombinant Antibody (clone 3F1) | ELISA, WB, IHC | Rabbit IgG |
| VS3-CJ947 | Rabbit Anti-STAT5A Recombinant Antibody (VS3-CJ947) | WB, ICC, IF, IHC, IP, FC | Rabbit IgG |
| VS3-CJ948 | Rabbit Anti-STAT5A (phospho Y694) Recombinant Antibody (VS3-CJ948) | WB, IHC | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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