Mouse Anti-TACR1 Recombinant Antibody (clone 12) (CAT#: FAMAB-0216JF)
Recombinant monoclonal antibody specially binds to the ligand-binding domain of the neurokinin 1 receptor for the neuropeptide substance P. This mouse anti-neurokinin 1 receptor antibody (clone 12) is useful to study the mechanism of various diseases of the central nervous system, sunch as schizophrenia, migraine, fibromyalgia.
Specific Inquiry
WB
Figure 1 Tacr1 expression and comparison of NF-κB activation in response to SP and tumor necrosis factor (TNF).
Immunoblot of Tacr1 expression in A549 cells transfected with Tacr1 (lane 1) or control vector (lane 2).
Williams, R., Zou, X., & Hoyle, G. W. (2007). Tachykinin-1 receptor stimulates proinflammatory gene expression in lung epithelial cells through activation of NF-κB via a Gq-dependent pathway. American Journal of Physiology-Lung Cellular and Molecular Physiology, 292(2), L430-L437.
ELISA
Figure 2 Cells were transfected with Tacr1, and IL-8 was measured in cell culture supernatants by ELISA.
SP treatment upregulated IL-8, and this effect was inhibited by cotransfection of a mutant IκB superrepressor lacking amino acids 1–36 that acts as a specific NF-κB inhibitor.
Williams, R., Zou, X., & Hoyle, G. W. (2007). Tachykinin-1 receptor stimulates proinflammatory gene expression in lung epithelial cells through activation of NF-κB via a Gq-dependent pathway. American Journal of Physiology-Lung Cellular and Molecular Physiology, 292(2), L430-L437.
IHC
Figure 3 TACR1 expression by IHC assay (×200).
This assay was compared with adjacent normal tissues.
Hongfeng, Z., Andong, J., Liwen, S., Mingping, B., Xiaowei, Y., Mingyong, L., & Aimin, Y. (2020). lncRNA RMRP knockdown suppress hepatocellular carcinoma biological activities via regulation miRNA‐206/TACR1. Journal of Cellular Biochemistry, 121(2), 1690-1702.
Specifications
- Immunogen
- PS5 (sequence HQSKELLRLGS) conjugate emulsified with Freund's complete adjuvant
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Rat TACR1
- Species Reactivity
- Rat
- Clone
- 12
- Applications
- WB, ELISA, IHC
- Related Disease
- Schizophrenia, Migraine, Fibromyalgia
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The TACR1 antibody has been reported in applications of Western Blot, Enzyme-linked Immunosorbent Assay, Immunohistochemistry.
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2516z | Mouse Anti-TACR1 Recombinant Antibody (clone 35G6) | WB, ELISA, IF, IP | Mouse IgG1, κ |
| FAMAB-0217JF | Mouse Anti-TACR1 Recombinant Antibody (clone 11) | WB, ELISA, IHC | Mouse IgG3 |
| FAMAB-0218JF | Mouse Anti-TACR1 Recombinant Antibody (clone 6) | WB, IHC | Mouse IgG2a |
| FAMAB-0219JF | Mouse Anti-TACR1 Recombinant Antibody (clone 17) | WB, Inhib | Mouse IgG1 |
| VS3-FY1422 | Recombinant Rabbit Anti-TACR1 Antibody (clone R05-3D1) | WB, IP | Rabbit IgG |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3490 | Hi-Affi™ Recombinant Rabbit Anti-TACR1 Monoclonal Antibody (DS3490AB) | IP, WB | IgG |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0216JF-F(E) | Mouse Anti-TACR1 Recombinant Antibody (clone 12); Fab Fragment | IHC, ELISA, FuncS | Mouse Fab |
| FAMAB-0217JF-F(E) | Mouse Anti-TACR1 Recombinant Antibody (clone 11); Fab Fragment | IHC, ELISA, FuncS | Mouse Fab |
| FAMAB-0218JF-F(E) | Mouse Anti-TACR1 Recombinant Antibody (clone 6); Fab Fragment | IHC, ELISA, FuncS | Mouse Fab |
| FAMAB-0219JF-F(E) | Mouse Anti-TACR1 Recombinant Antibody (clone 17); Fab Fragment | IHC, ELISA, FuncS | Mouse Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0216JF-S(P) | Mouse Anti-TACR1 Recombinant Antibody (clone 12); scFv Fragment | IHC, ELISA, FuncS | Mouse scFv |
| FAMAB-0217JF-S(P) | Mouse Anti-TACR1 Recombinant Antibody (clone 11); scFv Fragment | IHC, ELISA, FuncS | Mouse scFv |
| FAMAB-0218JF-S(P) | Mouse Anti-TACR1 Recombinant Antibody (clone 6); scFv Fragment | IHC, ELISA, FuncS | Mouse scFv |
| FAMAB-0219JF-S(P) | Mouse Anti-TACR1 Recombinant Antibody (clone 17); scFv Fragment | IHC, ELISA, FuncS | Mouse scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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