Human Anti-THY1 Recombinant Antibody (VS-0723-CJ26)
CAT#: VS-0723-CJ26
This human anti-THY1 antibody is a product that binds both soluble and cellularly expressed Thy1. This antibody scFv fragment had a KD of 3.4 nM for human Thy1 and 9.2 nM for mouse Thy1. This antibody has been tested for use in flow cytometry and immunofluorescence.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3733/992_A2_2_selected.jpg
Normal Tissue
Figure 2 IHC staining of human kidney
Immunohistochemical staining of human kidney shows moderate membranous positivity in cells in tubules.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68243/ihc_selected.jpg
Normal Tissue
Figure 3 IHC staining of human hippocampus
Immunohistochemical staining of human hippocampus shows moderate nuclear positivity in neuronal cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68244/ihc_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3733/156319_B_8_5.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (ASC diff, BJ, BJ hTERT+, fHDF/TERT166)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000154096-THY1
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Specifications
- Host Species
- Human
- Type
- Human IgG
- Specificity
- Human and mouse THY1
- Species Reactivity
- Human, Mouse
- Applications
- FC, IF
- Conjugate
- Unconjugated
- Related Disease
- Pancreatic ductal adenocarcinoma (PDAC)
Product Property
- Purification
- Protein A purified
- Purity
- >95%
- Buffer
- PBS
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody binding to soluble human and murine Thy1 and to Thy1-expressing cells was assessed by flow cytometry. Immunofluorescence staining of this antibody was performed with human Thy1 expression on vascular endothelial MS1 cells.
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7248 | Anti-Mouse THY1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7249 | Anti-Rat THY1 Immunohistochemistry Kit | IHC |
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