Human Anti-TREM2 Recombinant Antibody (clone 2) (CAT#: VS-0323-CJ59)

• Immunogen
• A TREM2 antigen comprising the Ig domain of human TREM2 (His19-Asp131) biotinylated on a C-terminal Avi tag

• Host Species
• Human

• Type
• Human IgG

• Specificity
• Human TREM2 ectodomain

• Species Reactivity
• Human

• Clone
• 2

• Applications
• Enzyme-linked Immunosorbent Assay, Surface Plasmon Resonance, Immunoassay

• Conjugate
• Unconjugated

• Related Disease
• Alzheimer's disease

• Purity
• >95% as determined by SDS-PAGE

• Buffer
• PBS

• Storage
• Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• ELISA: 1 ng/mL-4 ng/mL
  Surface Plasmon Resonance: 2 nM-55 nM

• Alternative Names
• PLOSL2; TREM-2; Trem2a; Trem2b; Trem2c

• Gene ID
• 54209

• UniProt ID
• Q9NZC2

• Cellular Localization
• Cell membrane; Secreted

• Post Translation Modifications
• Undergoes ectodomain shedding through proteolytic cleavage by ADAM10 and ADAM17 to produce a transmembrane segment, the TREM2 C-terminal fragment (TREM2-CTF), which is subsequently cleaved by gamma-secretase.

• Protein Refseq
• NP_001258750.1; NP_061838.1

• Function
• Forms a receptor signaling complex with TYROBP which mediates signaling and cell activation following ligand binding (PubMed:10799849).
  Acts as a receptor for amyloid-beta protein 42, a cleavage product of the amyloid-beta precursor protein APP, and mediates its uptake and degradation by microglia (PubMed:27477018, PubMed:29518356).
  Binding to amyloid-beta 42 mediates microglial activation, proliferation, migration, apoptosis and expression of pro-inflammatory cytokines, such as IL6R and CCL3, and the anti-inflammatory cytokine ARG1 (By similarity).
  Acts as a receptor for lipoprotein particles such as LDL, VLDL, and HDL and for apolipoproteins such as APOA1, APOA2, APOB, APOE, APOE2, APOE3, APOE4, and CLU and enhances their uptake in microglia (PubMed:27477018).
  Binds phospholipids (preferably anionic lipids) such as phosphatidylserine, phosphatidylethanolamine, phosphatidylglycerol and sphingomyelin (PubMed:29794134).
  Regulates microglial proliferation by acting as an upstream regulator of the Wnt/beta-catenin signaling cascade (By similarity).
  Required for microglial phagocytosis of apoptotic neurons (PubMed:24990881).
  Also required for microglial activation and phagocytosis of myelin debris after neuronal injury and of neuronal synapses during synapse elimination in the developing brain (By similarity).
  Regulates microglial chemotaxis and process outgrowth, and also the microglial response to oxidative stress and lipopolysaccharide (By similarity).
  It suppresses PI3K and NF-kappa-B signaling in response to lipopolysaccharide; thus promoting phagocytosis, suppressing pro-inflammatory cytokine and nitric oxide production, inhibiting apoptosis and increasing expression of IL10 and TGFB (By similarity).
  During oxidative stress, it promotes anti-apoptotic NF-kappa-B signaling and ERK signaling (By similarity).
  Plays a role in microglial MTOR activation and metabolism (By similarity).
  Regulates age-related changes in microglial numbers (PubMed:29752066).
  Triggers activation of the immune responses in macrophages and dendritic cells (PubMed:10799849).
  Mediates cytokine-induced formation of multinucleated giant cells which are formed by the fusion of macrophages (By similarity).
  In dendritic cells, it mediates up-regulation of chemokine receptor CCR7 and dendritic cell maturation and survival (PubMed:11602640).
  Involved in the positive regulation of osteoclast differentiation (PubMed:12925681).

Isotype Control

• MRO-A1587-YJ
• Human IgG Isotype control, Polyclonal

Secondary Antibody

• 2ND-024CQ
• Goat Anti-Human IgG (H&L) Antibody

• 2ND-025CQ
• Rabbit Anti-Human IgG (H&L) Antibody

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS

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